Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yheO and tusD overlap by 1 nucleotides tusD and tusD overlap by 1 nucleotides tusD and tusB are separated by 7 nucleotides tusB and rpsL are separated by 125 nucleotides rpsL and rpsG are separated by 96 nucleotides
HEPCGN_16130: yheO - Uncharacterized protein YheO, at 1,997,341 to 1,998,063
yheO
HEPCGN_16135: tusD - sulfurtransferase complex subunit TusD, at 1,998,063 to 1,998,449
tusD
HEPCGN_16140: tusD - sulfurtransferase complex subunit TusD, at 1,998,449 to 1,998,808
tusD
HEPCGN_16145: tusB - sulfurtransferase complex subunit TusB, at 1,998,816 to 1,999,103
tusB
HEPCGN_16150: rpsL - 30S ribosomal protein S12, at 1,999,229 to 1,999,603
rpsL
HEPCGN_16155: rpsG - 30S ribosomal protein S7, at 1,999,700 to 2,000,170
rpsG
Position (kb) 1998
1999
2000 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 1997.898 kb on + strand, within yheO at 1997.898 kb on + strand, within yheO at 1997.932 kb on + strand, within yheO at 1998.010 kb on + strand at 1998.016 kb on + strand at 1998.815 kb on + strand at 1999.120 kb on + strand at 1999.167 kb on + strand at 1999.185 kb on + strand at 1999.188 kb on + strand at 1999.196 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,997,898 + yheO HEPCGN_16130 0.77 +3.8 1,997,898 + yheO HEPCGN_16130 0.77 -0.1 1,997,932 + yheO HEPCGN_16130 0.82 -0.1 1,998,010 + +2.2 1,998,016 + -1.8 1,998,815 + +0.2 1,999,120 + -0.4 1,999,167 + +1.5 1,999,185 + -0.3 1,999,188 + -0.1 1,999,196 + +0.5
Or see this region's nucleotide sequence