Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt envZ and yiaG are separated by 31 nucleotides yiaG and HEPCGN_15860 are separated by 58 nucleotides HEPCGN_15860 and pckA are separated by 114 nucleotides
HEPCGN_15850: envZ - two-component system sensor histidine kinase EnvZ, at 1,942,436 to 1,943,788
envZ
HEPCGN_15855: yiaG - transcriptional regulator, at 1,943,820 to 1,944,116
yiaG
HEPCGN_15860: HEPCGN_15860 - toxin HigB-2, at 1,944,175 to 1,944,492
_15860
HEPCGN_15865: pckA - phosphoenolpyruvate carboxykinase (ATP), at 1,944,607 to 1,946,229
pckA
Position (kb)
1944
1945 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1943.229 kb on - strand, within envZ at 1943.249 kb on - strand, within envZ at 1943.295 kb on - strand, within envZ at 1943.379 kb on - strand, within envZ at 1943.547 kb on + strand, within envZ at 1943.584 kb on + strand, within envZ at 1943.584 kb on + strand, within envZ at 1943.584 kb on + strand, within envZ at 1943.584 kb on + strand, within envZ at 1943.585 kb on - strand, within envZ at 1943.585 kb on - strand, within envZ at 1943.585 kb on - strand, within envZ at 1943.585 kb on - strand, within envZ at 1943.585 kb on - strand, within envZ at 1943.585 kb on - strand, within envZ at 1943.787 kb on - strand at 1943.935 kb on + strand, within yiaG at 1943.935 kb on + strand, within yiaG at 1943.974 kb on - strand, within yiaG at 1943.974 kb on - strand, within yiaG at 1943.974 kb on - strand, within yiaG at 1944.000 kb on + strand, within yiaG at 1944.020 kb on + strand, within yiaG at 1944.196 kb on + strand at 1944.227 kb on - strand, within HEPCGN_15860 at 1944.286 kb on + strand, within HEPCGN_15860 at 1944.286 kb on + strand, within HEPCGN_15860 at 1944.296 kb on + strand, within HEPCGN_15860 at 1944.297 kb on - strand, within HEPCGN_15860 at 1944.376 kb on - strand, within HEPCGN_15860 at 1944.406 kb on + strand, within HEPCGN_15860 at 1944.406 kb on + strand, within HEPCGN_15860 at 1944.406 kb on + strand, within HEPCGN_15860 at 1944.407 kb on - strand, within HEPCGN_15860 at 1944.439 kb on + strand, within HEPCGN_15860 at 1944.446 kb on - strand, within HEPCGN_15860 at 1944.446 kb on - strand, within HEPCGN_15860 at 1944.492 kb on + strand at 1944.493 kb on - strand at 1944.493 kb on - strand at 1944.510 kb on - strand at 1944.516 kb on - strand at 1944.519 kb on + strand at 1944.520 kb on - strand at 1944.538 kb on + strand at 1944.553 kb on - strand at 1944.553 kb on - strand at 1944.609 kb on - strand at 1944.609 kb on - strand at 1944.629 kb on - strand at 1944.659 kb on + strand at 1944.660 kb on - strand at 1944.664 kb on - strand at 1944.731 kb on + strand at 1944.731 kb on + strand at 1944.731 kb on + strand at 1944.732 kb on - strand at 1944.732 kb on - strand at 1944.732 kb on - strand at 1944.732 kb on - strand at 1944.732 kb on - strand at 1944.763 kb on + strand at 1944.883 kb on - strand, within pckA at 1944.883 kb on - strand, within pckA at 1944.945 kb on + strand, within pckA at 1944.945 kb on + strand, within pckA at 1944.946 kb on - strand, within pckA at 1944.946 kb on - strand, within pckA at 1944.946 kb on - strand, within pckA at 1945.158 kb on + strand, within pckA at 1945.158 kb on + strand, within pckA at 1945.165 kb on - strand, within pckA at 1945.194 kb on + strand, within pckA at 1945.214 kb on + strand, within pckA at 1945.214 kb on + strand, within pckA at 1945.214 kb on + strand, within pckA at 1945.215 kb on - strand, within pckA at 1945.215 kb on - strand, within pckA at 1945.373 kb on + strand, within pckA at 1945.373 kb on + strand, within pckA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,943,229 - envZ HEPCGN_15850 0.59 -0.5 1,943,249 - envZ HEPCGN_15850 0.60 +0.1 1,943,295 - envZ HEPCGN_15850 0.63 -2.9 1,943,379 - envZ HEPCGN_15850 0.70 -0.0 1,943,547 + envZ HEPCGN_15850 0.82 -2.9 1,943,584 + envZ HEPCGN_15850 0.85 -0.7 1,943,584 + envZ HEPCGN_15850 0.85 +0.4 1,943,584 + envZ HEPCGN_15850 0.85 -0.1 1,943,584 + envZ HEPCGN_15850 0.85 -2.6 1,943,585 - envZ HEPCGN_15850 0.85 -0.0 1,943,585 - envZ HEPCGN_15850 0.85 +2.1 1,943,585 - envZ HEPCGN_15850 0.85 +0.5 1,943,585 - envZ HEPCGN_15850 0.85 -2.1 1,943,585 - envZ HEPCGN_15850 0.85 -0.5 1,943,585 - envZ HEPCGN_15850 0.85 +0.1 1,943,787 - +0.0 1,943,935 + yiaG HEPCGN_15855 0.39 -0.0 1,943,935 + yiaG HEPCGN_15855 0.39 +0.3 1,943,974 - yiaG HEPCGN_15855 0.52 +0.3 1,943,974 - yiaG HEPCGN_15855 0.52 +0.6 1,943,974 - yiaG HEPCGN_15855 0.52 -0.3 1,944,000 + yiaG HEPCGN_15855 0.61 -0.7 1,944,020 + yiaG HEPCGN_15855 0.67 +0.5 1,944,196 + -0.1 1,944,227 - HEPCGN_15860 0.16 +0.1 1,944,286 + HEPCGN_15860 0.35 -0.1 1,944,286 + HEPCGN_15860 0.35 -0.2 1,944,296 + HEPCGN_15860 0.38 +0.5 1,944,297 - HEPCGN_15860 0.38 -0.4 1,944,376 - HEPCGN_15860 0.63 -2.8 1,944,406 + HEPCGN_15860 0.73 -0.0 1,944,406 + HEPCGN_15860 0.73 +0.7 1,944,406 + HEPCGN_15860 0.73 +1.1 1,944,407 - HEPCGN_15860 0.73 +0.1 1,944,439 + HEPCGN_15860 0.83 +0.9 1,944,446 - HEPCGN_15860 0.85 -0.5 1,944,446 - HEPCGN_15860 0.85 +0.7 1,944,492 + -1.6 1,944,493 - +1.5 1,944,493 - -0.3 1,944,510 - -0.1 1,944,516 - +0.6 1,944,519 + -0.2 1,944,520 - +0.3 1,944,538 + -0.0 1,944,553 - +0.0 1,944,553 - -0.2 1,944,609 - +0.0 1,944,609 - +0.4 1,944,629 - -0.2 1,944,659 + +0.3 1,944,660 - +0.2 1,944,664 - +0.1 1,944,731 + +0.2 1,944,731 + -0.3 1,944,731 + +1.2 1,944,732 - +0.4 1,944,732 - -1.4 1,944,732 - -0.3 1,944,732 - -0.1 1,944,732 - -0.6 1,944,763 + -0.9 1,944,883 - pckA HEPCGN_15865 0.17 +0.4 1,944,883 - pckA HEPCGN_15865 0.17 +0.8 1,944,945 + pckA HEPCGN_15865 0.21 -1.2 1,944,945 + pckA HEPCGN_15865 0.21 -0.1 1,944,946 - pckA HEPCGN_15865 0.21 +0.6 1,944,946 - pckA HEPCGN_15865 0.21 -0.8 1,944,946 - pckA HEPCGN_15865 0.21 +0.5 1,945,158 + pckA HEPCGN_15865 0.34 -0.2 1,945,158 + pckA HEPCGN_15865 0.34 +0.3 1,945,165 - pckA HEPCGN_15865 0.34 -2.0 1,945,194 + pckA HEPCGN_15865 0.36 -0.3 1,945,214 + pckA HEPCGN_15865 0.37 +0.4 1,945,214 + pckA HEPCGN_15865 0.37 +0.1 1,945,214 + pckA HEPCGN_15865 0.37 -0.1 1,945,215 - pckA HEPCGN_15865 0.37 -0.4 1,945,215 - pckA HEPCGN_15865 0.37 -0.2 1,945,373 + pckA HEPCGN_15865 0.47 +0.1 1,945,373 + pckA HEPCGN_15865 0.47 -0.1
Or see this region's nucleotide sequence