Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glpG and glpR are separated by 16 nucleotides glpR and rtcR overlap by 19 nucleotides rtcR and rtcB are separated by 188 nucleotides
HEPCGN_15755: glpG - rhomboid family intramembrane serine protease GlpG, at 1,916,939 to 1,917,763
glpG
HEPCGN_15760: glpR - DeoR/GlpR family transcriptional regulator, at 1,917,780 to 1,918,538
glpR
HEPCGN_15765: rtcR - DNA-binding transcriptional regulator RtcR, at 1,918,520 to 1,920,118
rtcR
HEPCGN_15770: rtcB - RNA-splicing ligase RtcB, at 1,920,307 to 1,921,533
rtcB
Position (kb)
1918
1919
1920
1921 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1917.546 kb on - strand, within glpG at 1917.546 kb on - strand, within glpG at 1917.546 kb on - strand, within glpG at 1917.602 kb on + strand, within glpG at 1917.619 kb on - strand, within glpG at 1917.622 kb on + strand, within glpG at 1917.622 kb on + strand, within glpG at 1917.623 kb on - strand, within glpG at 1917.718 kb on - strand at 1917.761 kb on + strand at 1917.775 kb on + strand at 1917.806 kb on + strand at 1917.806 kb on + strand at 1917.807 kb on - strand at 1917.822 kb on - strand at 1917.834 kb on + strand at 1917.834 kb on + strand at 1917.835 kb on - strand at 1917.835 kb on - strand at 1917.835 kb on - strand at 1917.835 kb on - strand at 1917.884 kb on + strand, within glpR at 1918.158 kb on - strand, within glpR at 1918.256 kb on + strand, within glpR at 1918.434 kb on - strand, within glpR at 1918.453 kb on + strand, within glpR at 1918.453 kb on + strand, within glpR at 1918.453 kb on + strand, within glpR at 1918.453 kb on + strand, within glpR at 1918.453 kb on + strand, within glpR at 1918.454 kb on - strand, within glpR at 1918.517 kb on + strand at 1918.517 kb on + strand at 1918.578 kb on + strand at 1918.579 kb on - strand at 1918.579 kb on - strand at 1918.754 kb on - strand, within rtcR at 1918.783 kb on + strand, within rtcR at 1918.784 kb on - strand, within rtcR at 1918.785 kb on + strand, within rtcR at 1918.786 kb on - strand, within rtcR at 1918.951 kb on + strand, within rtcR at 1918.952 kb on - strand, within rtcR at 1918.999 kb on - strand, within rtcR at 1918.999 kb on - strand, within rtcR at 1919.077 kb on - strand, within rtcR at 1919.141 kb on - strand, within rtcR at 1919.262 kb on - strand, within rtcR at 1919.427 kb on - strand, within rtcR at 1919.626 kb on + strand, within rtcR at 1919.651 kb on - strand, within rtcR at 1919.708 kb on + strand, within rtcR at 1919.709 kb on - strand, within rtcR at 1919.723 kb on + strand, within rtcR at 1919.723 kb on + strand, within rtcR at 1919.727 kb on + strand, within rtcR at 1919.793 kb on - strand, within rtcR at 1919.796 kb on + strand, within rtcR at 1919.889 kb on - strand, within rtcR at 1919.889 kb on - strand, within rtcR at 1920.003 kb on - strand at 1920.021 kb on - strand at 1920.072 kb on + strand at 1920.112 kb on + strand at 1920.113 kb on - strand at 1920.121 kb on + strand at 1920.121 kb on + strand at 1920.122 kb on - strand at 1920.132 kb on - strand at 1920.272 kb on - strand at 1920.399 kb on + strand at 1920.400 kb on - strand at 1920.432 kb on + strand, within rtcB at 1920.452 kb on + strand, within rtcB at 1920.452 kb on + strand, within rtcB at 1920.452 kb on + strand, within rtcB at 1920.452 kb on + strand, within rtcB at 1920.452 kb on + strand, within rtcB at 1920.452 kb on + strand, within rtcB at 1920.453 kb on - strand, within rtcB at 1920.453 kb on - strand, within rtcB at 1920.453 kb on - strand, within rtcB at 1920.654 kb on + strand, within rtcB at 1920.654 kb on + strand, within rtcB at 1920.664 kb on - strand, within rtcB at 1920.765 kb on + strand, within rtcB at 1920.766 kb on - strand, within rtcB at 1920.771 kb on + strand, within rtcB at 1920.772 kb on - strand, within rtcB at 1920.775 kb on + strand, within rtcB at 1920.775 kb on + strand, within rtcB at 1920.776 kb on - strand, within rtcB at 1920.776 kb on - strand, within rtcB at 1920.776 kb on - strand, within rtcB at 1920.776 kb on - strand, within rtcB at 1920.776 kb on - strand, within rtcB at 1920.776 kb on - strand, within rtcB at 1920.776 kb on - strand, within rtcB at 1920.777 kb on + strand, within rtcB at 1920.852 kb on + strand, within rtcB at 1920.882 kb on + strand, within rtcB at 1920.883 kb on - strand, within rtcB at 1920.898 kb on + strand, within rtcB at 1920.899 kb on - strand, within rtcB at 1920.899 kb on - strand, within rtcB
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,917,546 - glpG HEPCGN_15755 0.74 -0.9 1,917,546 - glpG HEPCGN_15755 0.74 -0.5 1,917,546 - glpG HEPCGN_15755 0.74 -0.1 1,917,602 + glpG HEPCGN_15755 0.80 +0.8 1,917,619 - glpG HEPCGN_15755 0.82 -1.2 1,917,622 + glpG HEPCGN_15755 0.83 +0.7 1,917,622 + glpG HEPCGN_15755 0.83 -0.0 1,917,623 - glpG HEPCGN_15755 0.83 -1.3 1,917,718 - +0.1 1,917,761 + +0.3 1,917,775 + +0.0 1,917,806 + -0.1 1,917,806 + +0.1 1,917,807 - +0.2 1,917,822 - +0.5 1,917,834 + -0.2 1,917,834 + -1.0 1,917,835 - -0.9 1,917,835 - -0.5 1,917,835 - -1.0 1,917,835 - -0.9 1,917,884 + glpR HEPCGN_15760 0.14 -0.8 1,918,158 - glpR HEPCGN_15760 0.50 +0.5 1,918,256 + glpR HEPCGN_15760 0.63 -0.4 1,918,434 - glpR HEPCGN_15760 0.86 -0.9 1,918,453 + glpR HEPCGN_15760 0.89 -0.7 1,918,453 + glpR HEPCGN_15760 0.89 -1.0 1,918,453 + glpR HEPCGN_15760 0.89 -0.5 1,918,453 + glpR HEPCGN_15760 0.89 -1.4 1,918,453 + glpR HEPCGN_15760 0.89 -0.9 1,918,454 - glpR HEPCGN_15760 0.89 -0.3 1,918,517 + -0.5 1,918,517 + -1.0 1,918,578 + +0.6 1,918,579 - +0.3 1,918,579 - +0.2 1,918,754 - rtcR HEPCGN_15765 0.15 -1.7 1,918,783 + rtcR HEPCGN_15765 0.16 +2.5 1,918,784 - rtcR HEPCGN_15765 0.17 -0.4 1,918,785 + rtcR HEPCGN_15765 0.17 +0.6 1,918,786 - rtcR HEPCGN_15765 0.17 +0.1 1,918,951 + rtcR HEPCGN_15765 0.27 +1.0 1,918,952 - rtcR HEPCGN_15765 0.27 -0.6 1,918,999 - rtcR HEPCGN_15765 0.30 +0.4 1,918,999 - rtcR HEPCGN_15765 0.30 +0.4 1,919,077 - rtcR HEPCGN_15765 0.35 -0.9 1,919,141 - rtcR HEPCGN_15765 0.39 +0.3 1,919,262 - rtcR HEPCGN_15765 0.46 +0.1 1,919,427 - rtcR HEPCGN_15765 0.57 -0.2 1,919,626 + rtcR HEPCGN_15765 0.69 -1.0 1,919,651 - rtcR HEPCGN_15765 0.71 +0.6 1,919,708 + rtcR HEPCGN_15765 0.74 +0.5 1,919,709 - rtcR HEPCGN_15765 0.74 +2.9 1,919,723 + rtcR HEPCGN_15765 0.75 -0.2 1,919,723 + rtcR HEPCGN_15765 0.75 +0.1 1,919,727 + rtcR HEPCGN_15765 0.75 +0.3 1,919,793 - rtcR HEPCGN_15765 0.80 +0.3 1,919,796 + rtcR HEPCGN_15765 0.80 +0.4 1,919,889 - rtcR HEPCGN_15765 0.86 -0.4 1,919,889 - rtcR HEPCGN_15765 0.86 +0.9 1,920,003 - -0.2 1,920,021 - -0.6 1,920,072 + +0.2 1,920,112 + +0.3 1,920,113 - -0.3 1,920,121 + -0.2 1,920,121 + +0.5 1,920,122 - +0.7 1,920,132 - -1.4 1,920,272 - +0.2 1,920,399 + +0.2 1,920,400 - -0.2 1,920,432 + rtcB HEPCGN_15770 0.10 -0.3 1,920,452 + rtcB HEPCGN_15770 0.12 -0.0 1,920,452 + rtcB HEPCGN_15770 0.12 +0.2 1,920,452 + rtcB HEPCGN_15770 0.12 -0.6 1,920,452 + rtcB HEPCGN_15770 0.12 +0.2 1,920,452 + rtcB HEPCGN_15770 0.12 -0.9 1,920,452 + rtcB HEPCGN_15770 0.12 +1.1 1,920,453 - rtcB HEPCGN_15770 0.12 -0.7 1,920,453 - rtcB HEPCGN_15770 0.12 +0.5 1,920,453 - rtcB HEPCGN_15770 0.12 -0.2 1,920,654 + rtcB HEPCGN_15770 0.28 +0.8 1,920,654 + rtcB HEPCGN_15770 0.28 -0.0 1,920,664 - rtcB HEPCGN_15770 0.29 +0.2 1,920,765 + rtcB HEPCGN_15770 0.37 -0.2 1,920,766 - rtcB HEPCGN_15770 0.37 -0.2 1,920,771 + rtcB HEPCGN_15770 0.38 -0.1 1,920,772 - rtcB HEPCGN_15770 0.38 -0.8 1,920,775 + rtcB HEPCGN_15770 0.38 -0.3 1,920,775 + rtcB HEPCGN_15770 0.38 -0.2 1,920,776 - rtcB HEPCGN_15770 0.38 -0.4 1,920,776 - rtcB HEPCGN_15770 0.38 -0.7 1,920,776 - rtcB HEPCGN_15770 0.38 +0.2 1,920,776 - rtcB HEPCGN_15770 0.38 -1.0 1,920,776 - rtcB HEPCGN_15770 0.38 -0.2 1,920,776 - rtcB HEPCGN_15770 0.38 +0.2 1,920,776 - rtcB HEPCGN_15770 0.38 -0.6 1,920,777 + rtcB HEPCGN_15770 0.38 +0.3 1,920,852 + rtcB HEPCGN_15770 0.44 +0.3 1,920,882 + rtcB HEPCGN_15770 0.47 -0.5 1,920,883 - rtcB HEPCGN_15770 0.47 -0.3 1,920,898 + rtcB HEPCGN_15770 0.48 -0.4 1,920,899 - rtcB HEPCGN_15770 0.48 +2.8 1,920,899 - rtcB HEPCGN_15770 0.48 +1.8
Or see this region's nucleotide sequence