Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt priB and rpsF are separated by 6 nucleotides rpsF and yjfY are separated by 326 nucleotides yjfY and ulaF are separated by 128 nucleotides ulaF and ulaE overlap by 1 nucleotides
HEPCGN_10340: priB - primosomal replication protein N, at 814,227 to 814,541
priB
HEPCGN_10345: rpsF - 30S ribosomal protein S6, at 814,548 to 814,943
rpsF
HEPCGN_10350: yjfY - Uncharacterized protein YjfY, at 815,270 to 815,545
yjfY
HEPCGN_10355: ulaF - L-ribulose-5-phosphate 4-epimerase UlaF, at 815,674 to 816,360
ulaF
HEPCGN_10360: ulaE - L-ribulose-5-phosphate 3-epimerase UlaE, at 816,360 to 817,214
ulaE
Position (kb) 815
816 Strain fitness (log2 ratio) -2
-1
0
1 at 815.105 kb on + strand at 815.105 kb on + strand at 815.106 kb on - strand at 815.266 kb on - strand at 815.269 kb on + strand at 815.269 kb on + strand at 815.269 kb on + strand at 815.269 kb on + strand at 815.270 kb on - strand at 815.320 kb on + strand, within yjfY at 815.321 kb on - strand, within yjfY at 815.321 kb on - strand, within yjfY at 815.321 kb on - strand, within yjfY at 815.342 kb on - strand, within yjfY at 815.363 kb on - strand, within yjfY at 815.393 kb on + strand, within yjfY at 815.393 kb on + strand, within yjfY at 815.393 kb on + strand, within yjfY at 815.396 kb on - strand, within yjfY at 815.404 kb on + strand, within yjfY at 815.405 kb on - strand, within yjfY at 815.440 kb on - strand, within yjfY at 815.471 kb on + strand, within yjfY at 815.471 kb on + strand, within yjfY at 815.472 kb on - strand, within yjfY at 815.474 kb on + strand, within yjfY at 815.474 kb on + strand, within yjfY at 815.538 kb on - strand at 815.553 kb on + strand at 815.554 kb on - strand at 815.555 kb on + strand at 815.556 kb on - strand at 815.575 kb on + strand at 815.676 kb on - strand at 815.710 kb on - strand at 815.730 kb on + strand at 815.730 kb on + strand at 815.731 kb on - strand at 815.735 kb on + strand at 815.868 kb on - strand, within ulaF at 815.887 kb on - strand, within ulaF at 815.945 kb on + strand, within ulaF at 815.945 kb on + strand, within ulaF at 815.945 kb on + strand, within ulaF at 815.946 kb on - strand, within ulaF at 816.026 kb on - strand, within ulaF at 816.081 kb on + strand, within ulaF at 816.292 kb on - strand at 816.338 kb on - strand at 816.361 kb on + strand at 816.528 kb on + strand, within ulaE
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 815,105 + -0.3 815,105 + -0.4 815,106 - -0.3 815,266 - -0.3 815,269 + -0.1 815,269 + -0.1 815,269 + +0.3 815,269 + +0.4 815,270 - +0.6 815,320 + yjfY HEPCGN_10350 0.18 +0.9 815,321 - yjfY HEPCGN_10350 0.18 -0.2 815,321 - yjfY HEPCGN_10350 0.18 -0.5 815,321 - yjfY HEPCGN_10350 0.18 -1.7 815,342 - yjfY HEPCGN_10350 0.26 +0.5 815,363 - yjfY HEPCGN_10350 0.34 -0.0 815,393 + yjfY HEPCGN_10350 0.45 -0.8 815,393 + yjfY HEPCGN_10350 0.45 +0.8 815,393 + yjfY HEPCGN_10350 0.45 -0.2 815,396 - yjfY HEPCGN_10350 0.46 -0.2 815,404 + yjfY HEPCGN_10350 0.49 +0.0 815,405 - yjfY HEPCGN_10350 0.49 -0.8 815,440 - yjfY HEPCGN_10350 0.62 +0.4 815,471 + yjfY HEPCGN_10350 0.73 +0.2 815,471 + yjfY HEPCGN_10350 0.73 +0.1 815,472 - yjfY HEPCGN_10350 0.73 -0.3 815,474 + yjfY HEPCGN_10350 0.74 +0.4 815,474 + yjfY HEPCGN_10350 0.74 +0.7 815,538 - -0.2 815,553 + -1.2 815,554 - -2.5 815,555 + -0.4 815,556 - +0.2 815,575 + -0.2 815,676 - +0.5 815,710 - -0.4 815,730 + -0.5 815,730 + +0.5 815,731 - -0.4 815,735 + +0.5 815,868 - ulaF HEPCGN_10355 0.28 +0.3 815,887 - ulaF HEPCGN_10355 0.31 +0.8 815,945 + ulaF HEPCGN_10355 0.39 +0.1 815,945 + ulaF HEPCGN_10355 0.39 -0.9 815,945 + ulaF HEPCGN_10355 0.39 +0.8 815,946 - ulaF HEPCGN_10355 0.40 +0.7 816,026 - ulaF HEPCGN_10355 0.51 +0.8 816,081 + ulaF HEPCGN_10355 0.59 +0.7 816,292 - -0.2 816,338 - +0.5 816,361 + -0.3 816,528 + ulaE HEPCGN_10360 0.20 -0.6
Or see this region's nucleotide sequence