Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt osmY and prfC are separated by 392 nucleotides prfC and yjjG are separated by 90 nucleotides yjjG and rimI are separated by 14 nucleotides
HEPCGN_09280: osmY - molecular chaperone OsmY, at 600,897 to 601,502
osmY
HEPCGN_09285: prfC - peptide chain release factor 3, at 601,895 to 603,484
prfC
HEPCGN_09290: yjjG - pyrimidine 5'-nucleotidase, at 603,575 to 604,252
yjjG
HEPCGN_09295: rimI - ribosomal protein S18-alanine N-acetyltransferase, at 604,267 to 604,713
rimI
Position (kb) 601
602
603
604 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 600.988 kb on - strand, within osmY at 601.272 kb on - strand, within osmY at 601.358 kb on + strand, within osmY at 601.358 kb on + strand, within osmY at 601.371 kb on - strand, within osmY at 601.459 kb on - strand at 601.459 kb on - strand at 601.459 kb on - strand at 601.459 kb on - strand at 601.459 kb on - strand at 601.496 kb on + strand at 601.496 kb on + strand at 601.509 kb on - strand at 601.516 kb on + strand at 601.517 kb on - strand at 601.629 kb on + strand at 601.630 kb on - strand at 601.639 kb on + strand at 601.690 kb on + strand at 601.694 kb on + strand at 601.695 kb on - strand at 601.700 kb on + strand at 601.701 kb on - strand at 601.701 kb on - strand at 601.704 kb on + strand at 601.704 kb on + strand at 601.704 kb on + strand at 601.704 kb on + strand at 601.819 kb on - strand at 601.842 kb on + strand at 601.977 kb on + strand at 601.978 kb on - strand at 602.023 kb on + strand at 602.097 kb on + strand, within prfC at 602.235 kb on - strand, within prfC at 602.440 kb on + strand, within prfC at 602.511 kb on + strand, within prfC at 602.516 kb on - strand, within prfC at 602.533 kb on + strand, within prfC at 602.586 kb on - strand, within prfC at 602.618 kb on + strand, within prfC at 602.714 kb on - strand, within prfC at 602.714 kb on - strand, within prfC at 602.771 kb on - strand, within prfC at 602.908 kb on - strand, within prfC at 602.908 kb on - strand, within prfC at 602.908 kb on - strand, within prfC at 602.914 kb on - strand, within prfC at 602.926 kb on + strand, within prfC at 602.938 kb on - strand, within prfC at 603.065 kb on - strand, within prfC at 603.112 kb on + strand, within prfC at 603.128 kb on + strand, within prfC at 603.128 kb on + strand, within prfC at 603.129 kb on - strand, within prfC at 603.182 kb on - strand, within prfC at 603.187 kb on - strand, within prfC at 603.190 kb on + strand, within prfC at 603.232 kb on + strand, within prfC at 603.332 kb on - strand at 603.348 kb on - strand at 603.352 kb on + strand at 603.430 kb on + strand at 603.469 kb on - strand at 603.485 kb on - strand at 603.847 kb on - strand, within yjjG at 603.875 kb on + strand, within yjjG at 603.876 kb on - strand, within yjjG at 603.994 kb on + strand, within yjjG at 603.995 kb on - strand, within yjjG at 603.995 kb on - strand, within yjjG at 603.995 kb on - strand, within yjjG at 604.071 kb on - strand, within yjjG at 604.129 kb on - strand, within yjjG at 604.164 kb on + strand, within yjjG at 604.208 kb on - strand at 604.208 kb on - strand at 604.254 kb on - strand at 604.269 kb on - strand at 604.269 kb on - strand at 604.272 kb on + strand at 604.273 kb on - strand at 604.326 kb on - strand, within rimI at 604.328 kb on + strand, within rimI at 604.328 kb on + strand, within rimI at 604.329 kb on - strand, within rimI at 604.370 kb on + strand, within rimI
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 600,988 - osmY HEPCGN_09280 0.15 -3.8 601,272 - osmY HEPCGN_09280 0.62 -0.1 601,358 + osmY HEPCGN_09280 0.76 +0.6 601,358 + osmY HEPCGN_09280 0.76 +0.6 601,371 - osmY HEPCGN_09280 0.78 +0.2 601,459 - +0.1 601,459 - +0.1 601,459 - -0.4 601,459 - +0.2 601,459 - +0.4 601,496 + +0.1 601,496 + -0.9 601,509 - -0.1 601,516 + -0.2 601,517 - +0.3 601,629 + +0.5 601,630 - -0.3 601,639 + +0.2 601,690 + -0.6 601,694 + -2.7 601,695 - -1.0 601,700 + -0.2 601,701 - +0.6 601,701 - +0.8 601,704 + -0.8 601,704 + -0.1 601,704 + -0.2 601,704 + +0.2 601,819 - +0.0 601,842 + -0.0 601,977 + +0.7 601,978 - -0.3 602,023 + +0.0 602,097 + prfC HEPCGN_09285 0.13 +0.1 602,235 - prfC HEPCGN_09285 0.21 -0.9 602,440 + prfC HEPCGN_09285 0.34 -0.7 602,511 + prfC HEPCGN_09285 0.39 +0.2 602,516 - prfC HEPCGN_09285 0.39 -0.3 602,533 + prfC HEPCGN_09285 0.40 +0.3 602,586 - prfC HEPCGN_09285 0.43 -0.5 602,618 + prfC HEPCGN_09285 0.45 +0.9 602,714 - prfC HEPCGN_09285 0.52 -0.6 602,714 - prfC HEPCGN_09285 0.52 +0.5 602,771 - prfC HEPCGN_09285 0.55 +0.1 602,908 - prfC HEPCGN_09285 0.64 -0.5 602,908 - prfC HEPCGN_09285 0.64 -0.6 602,908 - prfC HEPCGN_09285 0.64 +0.2 602,914 - prfC HEPCGN_09285 0.64 +0.1 602,926 + prfC HEPCGN_09285 0.65 +0.1 602,938 - prfC HEPCGN_09285 0.66 -0.9 603,065 - prfC HEPCGN_09285 0.74 +0.4 603,112 + prfC HEPCGN_09285 0.77 +0.1 603,128 + prfC HEPCGN_09285 0.78 +0.1 603,128 + prfC HEPCGN_09285 0.78 -0.1 603,129 - prfC HEPCGN_09285 0.78 +0.8 603,182 - prfC HEPCGN_09285 0.81 -0.1 603,187 - prfC HEPCGN_09285 0.81 -1.8 603,190 + prfC HEPCGN_09285 0.81 -0.3 603,232 + prfC HEPCGN_09285 0.84 +0.0 603,332 - +0.3 603,348 - -1.2 603,352 + +0.1 603,430 + -0.8 603,469 - -0.7 603,485 - -0.8 603,847 - yjjG HEPCGN_09290 0.40 +0.1 603,875 + yjjG HEPCGN_09290 0.44 +0.2 603,876 - yjjG HEPCGN_09290 0.44 -0.2 603,994 + yjjG HEPCGN_09290 0.62 +1.1 603,995 - yjjG HEPCGN_09290 0.62 +0.2 603,995 - yjjG HEPCGN_09290 0.62 +0.0 603,995 - yjjG HEPCGN_09290 0.62 +0.1 604,071 - yjjG HEPCGN_09290 0.73 +0.0 604,129 - yjjG HEPCGN_09290 0.82 +0.5 604,164 + yjjG HEPCGN_09290 0.87 +0.4 604,208 - +0.3 604,208 - -0.5 604,254 - +0.7 604,269 - +0.4 604,269 - +0.1 604,272 + +1.0 604,273 - -0.1 604,326 - rimI HEPCGN_09295 0.13 +0.1 604,328 + rimI HEPCGN_09295 0.14 +1.0 604,328 + rimI HEPCGN_09295 0.14 +1.0 604,329 - rimI HEPCGN_09295 0.14 -1.5 604,370 + rimI HEPCGN_09295 0.23 -0.5
Or see this region's nucleotide sequence