Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mltD and ubiE are separated by 48 nucleotides ubiE and yafD are separated by 76 nucleotides yafD and yafC are separated by 261 nucleotides
HEPCGN_07925: mltD - murein transglycosylase D, at 322,928 to 324,148
mltD
HEPCGN_07930: ubiE - SAM-dependent methyltransferase, at 324,197 to 324,967
ubiE
HEPCGN_07935: yafD - endonuclease/exonuclease/phosphatase family protein, at 325,044 to 325,823
yafD
HEPCGN_07940: yafC - DNA-binding transcriptional regulator YafC, at 326,085 to 326,999
yafC
Position (kb) 325
326 Strain fitness (log2 ratio) -2
-1
0
1 at 324.061 kb on - strand at 324.194 kb on + strand at 324.195 kb on - strand at 324.195 kb on - strand at 324.195 kb on - strand at 324.209 kb on - strand at 324.219 kb on - strand at 324.219 kb on - strand at 324.229 kb on + strand at 324.230 kb on - strand at 324.230 kb on - strand at 324.317 kb on + strand, within ubiE at 324.318 kb on - strand, within ubiE at 324.318 kb on - strand, within ubiE at 324.321 kb on - strand, within ubiE at 324.372 kb on - strand, within ubiE at 324.376 kb on + strand, within ubiE at 324.377 kb on - strand, within ubiE at 324.398 kb on + strand, within ubiE at 324.399 kb on - strand, within ubiE at 324.419 kb on + strand, within ubiE at 324.444 kb on + strand, within ubiE at 324.463 kb on + strand, within ubiE at 324.488 kb on + strand, within ubiE at 324.524 kb on + strand, within ubiE at 324.524 kb on + strand, within ubiE at 324.525 kb on - strand, within ubiE at 324.525 kb on - strand, within ubiE at 324.525 kb on - strand, within ubiE at 324.525 kb on - strand, within ubiE at 324.625 kb on + strand, within ubiE at 324.632 kb on - strand, within ubiE at 324.753 kb on + strand, within ubiE at 324.754 kb on - strand, within ubiE at 324.825 kb on - strand, within ubiE at 324.825 kb on - strand, within ubiE at 324.825 kb on - strand, within ubiE at 324.890 kb on + strand, within ubiE at 324.890 kb on + strand, within ubiE at 324.906 kb on + strand at 324.907 kb on - strand at 324.938 kb on + strand at 324.938 kb on + strand at 324.939 kb on - strand at 324.939 kb on - strand at 324.981 kb on + strand at 325.040 kb on + strand at 325.041 kb on - strand at 325.041 kb on - strand at 325.081 kb on - strand at 325.114 kb on - strand at 325.237 kb on + strand, within yafD at 325.238 kb on - strand, within yafD at 325.240 kb on - strand, within yafD at 325.240 kb on - strand, within yafD at 325.257 kb on - strand, within yafD at 325.332 kb on + strand, within yafD at 325.333 kb on - strand, within yafD at 325.340 kb on + strand, within yafD at 325.341 kb on - strand, within yafD at 325.343 kb on + strand, within yafD at 325.380 kb on + strand, within yafD at 325.380 kb on + strand, within yafD at 325.380 kb on + strand, within yafD at 325.381 kb on - strand, within yafD at 325.383 kb on - strand, within yafD at 325.386 kb on - strand, within yafD at 325.486 kb on + strand, within yafD at 325.486 kb on + strand, within yafD at 325.487 kb on - strand, within yafD at 325.552 kb on - strand, within yafD at 325.570 kb on + strand, within yafD at 325.570 kb on + strand, within yafD at 325.575 kb on - strand, within yafD at 325.593 kb on + strand, within yafD at 325.594 kb on - strand, within yafD at 325.624 kb on - strand, within yafD at 325.690 kb on + strand, within yafD at 325.691 kb on - strand, within yafD at 325.692 kb on + strand, within yafD at 325.692 kb on + strand, within yafD at 325.692 kb on + strand, within yafD at 325.693 kb on - strand, within yafD at 325.817 kb on - strand at 325.866 kb on + strand at 325.867 kb on - strand at 325.898 kb on - strand at 325.904 kb on - strand at 325.940 kb on + strand at 325.943 kb on - strand at 325.943 kb on - strand at 325.953 kb on - strand at 325.953 kb on - strand at 326.000 kb on + strand at 326.014 kb on + strand at 326.015 kb on - strand at 326.164 kb on + strand at 326.164 kb on + strand at 326.255 kb on + strand, within yafC at 326.280 kb on + strand, within yafC at 326.293 kb on + strand, within yafC at 326.351 kb on + strand, within yafC at 326.352 kb on - strand, within yafC at 326.421 kb on - strand, within yafC at 326.421 kb on - strand, within yafC at 326.565 kb on - strand, within yafC at 326.600 kb on + strand, within yafC at 326.648 kb on + strand, within yafC at 326.649 kb on - strand, within yafC at 326.685 kb on - strand, within yafC at 326.716 kb on + strand, within yafC at 326.716 kb on + strand, within yafC at 326.716 kb on + strand, within yafC at 326.716 kb on + strand, within yafC at 326.716 kb on + strand, within yafC at 326.717 kb on - strand, within yafC at 326.717 kb on - strand, within yafC at 326.747 kb on + strand, within yafC at 326.809 kb on - strand, within yafC at 326.809 kb on - strand, within yafC
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 324,061 - -0.7 324,194 + +0.2 324,195 - -2.4 324,195 - -0.8 324,195 - +0.0 324,209 - +0.2 324,219 - +0.5 324,219 - -0.9 324,229 + +0.0 324,230 - -0.6 324,230 - -0.3 324,317 + ubiE HEPCGN_07930 0.16 -0.5 324,318 - ubiE HEPCGN_07930 0.16 +0.5 324,318 - ubiE HEPCGN_07930 0.16 +0.4 324,321 - ubiE HEPCGN_07930 0.16 +0.1 324,372 - ubiE HEPCGN_07930 0.23 -0.3 324,376 + ubiE HEPCGN_07930 0.23 -0.4 324,377 - ubiE HEPCGN_07930 0.23 -1.2 324,398 + ubiE HEPCGN_07930 0.26 +0.1 324,399 - ubiE HEPCGN_07930 0.26 +0.4 324,419 + ubiE HEPCGN_07930 0.29 -0.2 324,444 + ubiE HEPCGN_07930 0.32 +0.2 324,463 + ubiE HEPCGN_07930 0.35 -0.2 324,488 + ubiE HEPCGN_07930 0.38 -0.5 324,524 + ubiE HEPCGN_07930 0.42 +0.9 324,524 + ubiE HEPCGN_07930 0.42 -0.3 324,525 - ubiE HEPCGN_07930 0.43 -0.1 324,525 - ubiE HEPCGN_07930 0.43 +0.1 324,525 - ubiE HEPCGN_07930 0.43 +0.3 324,525 - ubiE HEPCGN_07930 0.43 +0.2 324,625 + ubiE HEPCGN_07930 0.56 -0.4 324,632 - ubiE HEPCGN_07930 0.56 +1.1 324,753 + ubiE HEPCGN_07930 0.72 -0.5 324,754 - ubiE HEPCGN_07930 0.72 -0.8 324,825 - ubiE HEPCGN_07930 0.81 +0.4 324,825 - ubiE HEPCGN_07930 0.81 +0.2 324,825 - ubiE HEPCGN_07930 0.81 -1.5 324,890 + ubiE HEPCGN_07930 0.90 -0.1 324,890 + ubiE HEPCGN_07930 0.90 -0.2 324,906 + -0.3 324,907 - +1.1 324,938 + +0.1 324,938 + -0.3 324,939 - -0.3 324,939 - +0.9 324,981 + -0.9 325,040 + +1.1 325,041 - +0.1 325,041 - -0.1 325,081 - +0.7 325,114 - +0.1 325,237 + yafD HEPCGN_07935 0.25 -0.1 325,238 - yafD HEPCGN_07935 0.25 +0.1 325,240 - yafD HEPCGN_07935 0.25 +0.2 325,240 - yafD HEPCGN_07935 0.25 -1.3 325,257 - yafD HEPCGN_07935 0.27 +0.3 325,332 + yafD HEPCGN_07935 0.37 -0.0 325,333 - yafD HEPCGN_07935 0.37 +0.5 325,340 + yafD HEPCGN_07935 0.38 -0.4 325,341 - yafD HEPCGN_07935 0.38 +0.8 325,343 + yafD HEPCGN_07935 0.38 -0.4 325,380 + yafD HEPCGN_07935 0.43 -0.3 325,380 + yafD HEPCGN_07935 0.43 -1.2 325,380 + yafD HEPCGN_07935 0.43 +0.4 325,381 - yafD HEPCGN_07935 0.43 -0.1 325,383 - yafD HEPCGN_07935 0.43 +0.1 325,386 - yafD HEPCGN_07935 0.44 +1.5 325,486 + yafD HEPCGN_07935 0.57 -0.7 325,486 + yafD HEPCGN_07935 0.57 +0.2 325,487 - yafD HEPCGN_07935 0.57 -1.7 325,552 - yafD HEPCGN_07935 0.65 +1.4 325,570 + yafD HEPCGN_07935 0.67 -0.2 325,570 + yafD HEPCGN_07935 0.67 +0.1 325,575 - yafD HEPCGN_07935 0.68 +0.7 325,593 + yafD HEPCGN_07935 0.70 +0.7 325,594 - yafD HEPCGN_07935 0.71 +0.2 325,624 - yafD HEPCGN_07935 0.74 +0.5 325,690 + yafD HEPCGN_07935 0.83 +0.2 325,691 - yafD HEPCGN_07935 0.83 -1.1 325,692 + yafD HEPCGN_07935 0.83 +0.3 325,692 + yafD HEPCGN_07935 0.83 +0.2 325,692 + yafD HEPCGN_07935 0.83 -0.1 325,693 - yafD HEPCGN_07935 0.83 +0.4 325,817 - +0.1 325,866 + +0.0 325,867 - +0.3 325,898 - -0.4 325,904 - -0.0 325,940 + +0.4 325,943 - +1.2 325,943 - +0.9 325,953 - +1.1 325,953 - +0.1 326,000 + +0.0 326,014 + -1.7 326,015 - -0.8 326,164 + -1.8 326,164 + -0.3 326,255 + yafC HEPCGN_07940 0.19 -1.0 326,280 + yafC HEPCGN_07940 0.21 +0.0 326,293 + yafC HEPCGN_07940 0.23 -0.8 326,351 + yafC HEPCGN_07940 0.29 +0.2 326,352 - yafC HEPCGN_07940 0.29 -0.9 326,421 - yafC HEPCGN_07940 0.37 -0.2 326,421 - yafC HEPCGN_07940 0.37 +0.3 326,565 - yafC HEPCGN_07940 0.52 +1.6 326,600 + yafC HEPCGN_07940 0.56 -0.9 326,648 + yafC HEPCGN_07940 0.62 -1.1 326,649 - yafC HEPCGN_07940 0.62 -1.3 326,685 - yafC HEPCGN_07940 0.66 -1.2 326,716 + yafC HEPCGN_07940 0.69 -0.3 326,716 + yafC HEPCGN_07940 0.69 -1.9 326,716 + yafC HEPCGN_07940 0.69 +0.2 326,716 + yafC HEPCGN_07940 0.69 -0.4 326,716 + yafC HEPCGN_07940 0.69 -0.1 326,717 - yafC HEPCGN_07940 0.69 +0.5 326,717 - yafC HEPCGN_07940 0.69 -2.4 326,747 + yafC HEPCGN_07940 0.72 -0.3 326,809 - yafC HEPCGN_07940 0.79 -0.3 326,809 - yafC HEPCGN_07940 0.79 -0.4
Or see this region's nucleotide sequence