Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yafS and gloB are separated by 33 nucleotides gloB and mltD are separated by 209 nucleotides mltD and ubiE are separated by 48 nucleotides ubiE and yafD are separated by 76 nucleotides
HEPCGN_07915: yafS - Uncharacterized protein YafS, at 321,207 to 321,929
yafS
HEPCGN_07920: gloB - hydroxyacylglutathione hydrolase, at 321,963 to 322,718
gloB
HEPCGN_07925: mltD - murein transglycosylase D, at 322,928 to 324,148
mltD
HEPCGN_07930: ubiE - SAM-dependent methyltransferase, at 324,197 to 324,967
ubiE
HEPCGN_07935: yafD - endonuclease/exonuclease/phosphatase family protein, at 325,044 to 325,823
yafD
Position (kb) 322
323
324
325 Strain fitness (log2 ratio) -2
-1
0
1
2 at 321.957 kb on + strand at 321.957 kb on + strand at 321.957 kb on + strand at 321.958 kb on - strand at 321.963 kb on - strand at 321.971 kb on + strand at 321.972 kb on - strand at 321.999 kb on + strand at 321.999 kb on + strand at 321.999 kb on + strand at 321.999 kb on + strand at 322.000 kb on - strand at 322.000 kb on - strand at 322.000 kb on - strand at 322.108 kb on + strand, within gloB at 322.186 kb on - strand, within gloB at 322.186 kb on - strand, within gloB at 322.186 kb on - strand, within gloB at 322.208 kb on + strand, within gloB at 322.209 kb on - strand, within gloB at 322.304 kb on + strand, within gloB at 322.305 kb on - strand, within gloB at 322.444 kb on + strand, within gloB at 322.461 kb on + strand, within gloB at 322.565 kb on - strand, within gloB at 322.710 kb on - strand at 322.710 kb on - strand at 322.945 kb on + strand at 323.039 kb on + strand at 323.044 kb on - strand at 323.050 kb on + strand, within mltD at 323.091 kb on - strand, within mltD at 323.092 kb on + strand, within mltD at 323.092 kb on + strand, within mltD at 323.092 kb on + strand, within mltD at 323.092 kb on + strand, within mltD at 323.092 kb on + strand, within mltD at 323.093 kb on - strand, within mltD at 323.093 kb on - strand, within mltD at 323.093 kb on - strand, within mltD at 323.093 kb on - strand, within mltD at 323.097 kb on - strand, within mltD at 323.097 kb on - strand, within mltD at 323.146 kb on - strand, within mltD at 323.148 kb on + strand, within mltD at 323.148 kb on + strand, within mltD at 323.148 kb on + strand, within mltD at 323.149 kb on - strand, within mltD at 323.149 kb on - strand, within mltD at 323.157 kb on + strand, within mltD at 323.243 kb on + strand, within mltD at 323.373 kb on - strand, within mltD at 323.373 kb on - strand, within mltD at 323.374 kb on + strand, within mltD at 323.375 kb on - strand, within mltD at 323.407 kb on + strand, within mltD at 323.477 kb on + strand, within mltD at 323.478 kb on - strand, within mltD at 323.794 kb on + strand, within mltD at 323.795 kb on - strand, within mltD at 323.816 kb on + strand, within mltD at 323.824 kb on - strand, within mltD at 323.953 kb on + strand, within mltD at 323.954 kb on - strand, within mltD at 323.989 kb on + strand, within mltD at 323.990 kb on - strand, within mltD at 323.990 kb on - strand, within mltD at 323.990 kb on - strand, within mltD at 323.990 kb on - strand, within mltD at 323.990 kb on - strand, within mltD at 323.990 kb on - strand, within mltD at 324.061 kb on - strand at 324.194 kb on + strand at 324.195 kb on - strand at 324.195 kb on - strand at 324.195 kb on - strand at 324.209 kb on - strand at 324.219 kb on - strand at 324.219 kb on - strand at 324.229 kb on + strand at 324.230 kb on - strand at 324.230 kb on - strand at 324.317 kb on + strand, within ubiE at 324.318 kb on - strand, within ubiE at 324.318 kb on - strand, within ubiE at 324.321 kb on - strand, within ubiE at 324.372 kb on - strand, within ubiE at 324.376 kb on + strand, within ubiE at 324.377 kb on - strand, within ubiE at 324.398 kb on + strand, within ubiE at 324.399 kb on - strand, within ubiE at 324.419 kb on + strand, within ubiE at 324.444 kb on + strand, within ubiE at 324.463 kb on + strand, within ubiE at 324.488 kb on + strand, within ubiE at 324.524 kb on + strand, within ubiE at 324.524 kb on + strand, within ubiE at 324.525 kb on - strand, within ubiE at 324.525 kb on - strand, within ubiE at 324.525 kb on - strand, within ubiE at 324.525 kb on - strand, within ubiE at 324.625 kb on + strand, within ubiE at 324.632 kb on - strand, within ubiE at 324.753 kb on + strand, within ubiE at 324.754 kb on - strand, within ubiE at 324.825 kb on - strand, within ubiE at 324.825 kb on - strand, within ubiE at 324.825 kb on - strand, within ubiE at 324.890 kb on + strand, within ubiE at 324.890 kb on + strand, within ubiE at 324.906 kb on + strand at 324.907 kb on - strand at 324.938 kb on + strand at 324.938 kb on + strand at 324.939 kb on - strand at 324.939 kb on - strand at 324.981 kb on + strand at 325.040 kb on + strand at 325.041 kb on - strand at 325.041 kb on - strand at 325.081 kb on - strand at 325.114 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 321,957 + +1.2 321,957 + +0.3 321,957 + -0.5 321,958 - +1.3 321,963 - -0.0 321,971 + -0.1 321,972 - +0.2 321,999 + +0.5 321,999 + -1.1 321,999 + +0.2 321,999 + -1.6 322,000 - -0.5 322,000 - -0.2 322,000 - +0.8 322,108 + gloB HEPCGN_07920 0.19 -0.5 322,186 - gloB HEPCGN_07920 0.29 -0.0 322,186 - gloB HEPCGN_07920 0.29 -0.4 322,186 - gloB HEPCGN_07920 0.29 +0.3 322,208 + gloB HEPCGN_07920 0.32 +0.1 322,209 - gloB HEPCGN_07920 0.33 +2.2 322,304 + gloB HEPCGN_07920 0.45 +0.4 322,305 - gloB HEPCGN_07920 0.45 -0.2 322,444 + gloB HEPCGN_07920 0.64 +0.5 322,461 + gloB HEPCGN_07920 0.66 +0.2 322,565 - gloB HEPCGN_07920 0.80 -0.1 322,710 - -0.4 322,710 - +1.8 322,945 + -0.1 323,039 + +0.9 323,044 - -0.4 323,050 + mltD HEPCGN_07925 0.10 +0.9 323,091 - mltD HEPCGN_07925 0.13 -0.1 323,092 + mltD HEPCGN_07925 0.13 +1.0 323,092 + mltD HEPCGN_07925 0.13 -0.4 323,092 + mltD HEPCGN_07925 0.13 -0.8 323,092 + mltD HEPCGN_07925 0.13 -0.6 323,092 + mltD HEPCGN_07925 0.13 -0.1 323,093 - mltD HEPCGN_07925 0.14 +0.8 323,093 - mltD HEPCGN_07925 0.14 +0.5 323,093 - mltD HEPCGN_07925 0.14 +0.4 323,093 - mltD HEPCGN_07925 0.14 -0.6 323,097 - mltD HEPCGN_07925 0.14 -0.3 323,097 - mltD HEPCGN_07925 0.14 +0.0 323,146 - mltD HEPCGN_07925 0.18 -0.9 323,148 + mltD HEPCGN_07925 0.18 -0.2 323,148 + mltD HEPCGN_07925 0.18 -0.5 323,148 + mltD HEPCGN_07925 0.18 -0.5 323,149 - mltD HEPCGN_07925 0.18 -0.9 323,149 - mltD HEPCGN_07925 0.18 +0.1 323,157 + mltD HEPCGN_07925 0.19 -0.2 323,243 + mltD HEPCGN_07925 0.26 +1.2 323,373 - mltD HEPCGN_07925 0.36 -1.4 323,373 - mltD HEPCGN_07925 0.36 +0.5 323,374 + mltD HEPCGN_07925 0.37 +0.4 323,375 - mltD HEPCGN_07925 0.37 -0.3 323,407 + mltD HEPCGN_07925 0.39 -0.5 323,477 + mltD HEPCGN_07925 0.45 +0.7 323,478 - mltD HEPCGN_07925 0.45 +0.5 323,794 + mltD HEPCGN_07925 0.71 +0.1 323,795 - mltD HEPCGN_07925 0.71 +0.1 323,816 + mltD HEPCGN_07925 0.73 +0.5 323,824 - mltD HEPCGN_07925 0.73 -0.1 323,953 + mltD HEPCGN_07925 0.84 -0.4 323,954 - mltD HEPCGN_07925 0.84 +0.5 323,989 + mltD HEPCGN_07925 0.87 -0.2 323,990 - mltD HEPCGN_07925 0.87 -2.2 323,990 - mltD HEPCGN_07925 0.87 -0.2 323,990 - mltD HEPCGN_07925 0.87 -0.5 323,990 - mltD HEPCGN_07925 0.87 +0.3 323,990 - mltD HEPCGN_07925 0.87 -0.2 323,990 - mltD HEPCGN_07925 0.87 +0.2 324,061 - -0.7 324,194 + +0.2 324,195 - -2.4 324,195 - -0.8 324,195 - +0.0 324,209 - +0.2 324,219 - +0.5 324,219 - -0.9 324,229 + +0.0 324,230 - -0.6 324,230 - -0.3 324,317 + ubiE HEPCGN_07930 0.16 -0.5 324,318 - ubiE HEPCGN_07930 0.16 +0.5 324,318 - ubiE HEPCGN_07930 0.16 +0.4 324,321 - ubiE HEPCGN_07930 0.16 +0.1 324,372 - ubiE HEPCGN_07930 0.23 -0.3 324,376 + ubiE HEPCGN_07930 0.23 -0.4 324,377 - ubiE HEPCGN_07930 0.23 -1.2 324,398 + ubiE HEPCGN_07930 0.26 +0.1 324,399 - ubiE HEPCGN_07930 0.26 +0.4 324,419 + ubiE HEPCGN_07930 0.29 -0.2 324,444 + ubiE HEPCGN_07930 0.32 +0.2 324,463 + ubiE HEPCGN_07930 0.35 -0.2 324,488 + ubiE HEPCGN_07930 0.38 -0.5 324,524 + ubiE HEPCGN_07930 0.42 +0.9 324,524 + ubiE HEPCGN_07930 0.42 -0.3 324,525 - ubiE HEPCGN_07930 0.43 -0.1 324,525 - ubiE HEPCGN_07930 0.43 +0.1 324,525 - ubiE HEPCGN_07930 0.43 +0.3 324,525 - ubiE HEPCGN_07930 0.43 +0.2 324,625 + ubiE HEPCGN_07930 0.56 -0.4 324,632 - ubiE HEPCGN_07930 0.56 +1.1 324,753 + ubiE HEPCGN_07930 0.72 -0.5 324,754 - ubiE HEPCGN_07930 0.72 -0.8 324,825 - ubiE HEPCGN_07930 0.81 +0.4 324,825 - ubiE HEPCGN_07930 0.81 +0.2 324,825 - ubiE HEPCGN_07930 0.81 -1.5 324,890 + ubiE HEPCGN_07930 0.90 -0.1 324,890 + ubiE HEPCGN_07930 0.90 -0.2 324,906 + -0.3 324,907 - +1.1 324,938 + +0.1 324,938 + -0.3 324,939 - -0.3 324,939 - +0.9 324,981 + -0.9 325,040 + +1.1 325,041 - +0.1 325,041 - -0.1 325,081 - +0.7 325,114 - +0.1
Or see this region's nucleotide sequence