Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rsxC and rsxB overlap by 8 nucleotides rsxB and rsxA overlap by 1 nucleotides rsxA and ydgK are separated by 76 nucleotides ydgK and ydgT are separated by 85 nucleotides
HEPCGN_05930: rsxC - electron transport complex subunit RsxC, at 1,056,150 to 1,058,372
rsxC
HEPCGN_05935: rsxB - electron transport complex subunit RsxB, at 1,058,365 to 1,058,943
rsxB
HEPCGN_05940: rsxA - electron transport complex subunit RsxA, at 1,058,943 to 1,059,524
rsxA
HEPCGN_05945: ydgK - Inner membrane protein YdgK, at 1,059,601 to 1,060,041
ydgK
HEPCGN_05950: ydgT - transcription modulator YdgT, at 1,060,127 to 1,060,342
ydgT
Position (kb)
1058
1059
1060 Strain fitness (log2 ratio)
-2
-1
0
1 at 1057.953 kb on - strand, within rsxC at 1057.969 kb on - strand, within rsxC at 1058.045 kb on + strand, within rsxC at 1058.084 kb on + strand, within rsxC at 1058.085 kb on - strand, within rsxC at 1058.239 kb on - strand at 1058.361 kb on + strand at 1058.366 kb on + strand at 1058.367 kb on - strand at 1058.488 kb on + strand, within rsxB at 1058.489 kb on - strand, within rsxB at 1058.523 kb on + strand, within rsxB at 1058.524 kb on - strand, within rsxB at 1058.575 kb on - strand, within rsxB at 1058.581 kb on - strand, within rsxB at 1058.593 kb on - strand, within rsxB at 1058.676 kb on + strand, within rsxB at 1058.944 kb on + strand at 1058.963 kb on + strand at 1058.972 kb on + strand at 1058.988 kb on - strand at 1058.988 kb on - strand at 1059.197 kb on + strand, within rsxA at 1059.366 kb on - strand, within rsxA at 1059.480 kb on - strand at 1059.553 kb on - strand at 1059.587 kb on + strand at 1059.588 kb on - strand at 1059.594 kb on - strand at 1059.603 kb on - strand at 1059.678 kb on + strand, within ydgK at 1059.768 kb on + strand, within ydgK at 1059.769 kb on - strand, within ydgK at 1059.769 kb on - strand, within ydgK at 1059.769 kb on - strand, within ydgK at 1059.771 kb on + strand, within ydgK at 1059.773 kb on + strand, within ydgK at 1059.774 kb on - strand, within ydgK at 1059.963 kb on + strand, within ydgK at 1059.968 kb on - strand, within ydgK at 1060.041 kb on + strand at 1060.042 kb on - strand at 1060.084 kb on - strand at 1060.095 kb on + strand at 1060.095 kb on + strand at 1060.097 kb on + strand at 1060.106 kb on + strand at 1060.107 kb on - strand at 1060.225 kb on - strand, within ydgT at 1060.229 kb on - strand, within ydgT at 1060.342 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,057,953 - rsxC HEPCGN_05930 0.81 +1.1 1,057,969 - rsxC HEPCGN_05930 0.82 +0.4 1,058,045 + rsxC HEPCGN_05930 0.85 -1.3 1,058,084 + rsxC HEPCGN_05930 0.87 -1.1 1,058,085 - rsxC HEPCGN_05930 0.87 +0.7 1,058,239 - -1.4 1,058,361 + -0.0 1,058,366 + -0.1 1,058,367 - -0.5 1,058,488 + rsxB HEPCGN_05935 0.21 +0.4 1,058,489 - rsxB HEPCGN_05935 0.21 -0.6 1,058,523 + rsxB HEPCGN_05935 0.27 -1.7 1,058,524 - rsxB HEPCGN_05935 0.27 +0.2 1,058,575 - rsxB HEPCGN_05935 0.36 -0.9 1,058,581 - rsxB HEPCGN_05935 0.37 -0.7 1,058,593 - rsxB HEPCGN_05935 0.39 -0.0 1,058,676 + rsxB HEPCGN_05935 0.54 -1.5 1,058,944 + -0.5 1,058,963 + +1.4 1,058,972 + +1.6 1,058,988 - -0.1 1,058,988 - -0.2 1,059,197 + rsxA HEPCGN_05940 0.44 +0.3 1,059,366 - rsxA HEPCGN_05940 0.73 -0.1 1,059,480 - -0.2 1,059,553 - -1.0 1,059,587 + +1.0 1,059,588 - -0.2 1,059,594 - -1.3 1,059,603 - +0.1 1,059,678 + ydgK HEPCGN_05945 0.17 +0.0 1,059,768 + ydgK HEPCGN_05945 0.38 -2.3 1,059,769 - ydgK HEPCGN_05945 0.38 -1.3 1,059,769 - ydgK HEPCGN_05945 0.38 -0.4 1,059,769 - ydgK HEPCGN_05945 0.38 -0.5 1,059,771 + ydgK HEPCGN_05945 0.39 +0.1 1,059,773 + ydgK HEPCGN_05945 0.39 -0.5 1,059,774 - ydgK HEPCGN_05945 0.39 +0.2 1,059,963 + ydgK HEPCGN_05945 0.82 +0.8 1,059,968 - ydgK HEPCGN_05945 0.83 +1.2 1,060,041 + -0.5 1,060,042 - -0.2 1,060,084 - +0.3 1,060,095 + +0.2 1,060,095 + -0.7 1,060,097 + +0.8 1,060,106 + +0.4 1,060,107 - -0.8 1,060,225 - ydgT HEPCGN_05950 0.45 +0.3 1,060,229 - ydgT HEPCGN_05950 0.47 -0.7 1,060,342 + -2.0
Or see this region's nucleotide sequence