Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sufS and sufE are separated by 12 nucleotides sufE and ldtE are separated by 148 nucleotides ldtE and lpp are separated by 62 nucleotides lpp and pykF are separated by 310 nucleotides
HEPCGN_05655: sufS - cysteine desulfurase SufS, at 1,004,769 to 1,005,989
sufS
HEPCGN_05660: sufE - cysteine desulfuration protein SufE, at 1,006,002 to 1,006,418
sufE
HEPCGN_05665: ldtE - L,D-transpeptidase LdtE, at 1,006,567 to 1,007,571
ldtE
HEPCGN_05670: lpp - murein lipoprotein Lpp, at 1,007,634 to 1,007,870
lpp
HEPCGN_05675: pykF - pyruvate kinase PykF, at 1,008,181 to 1,009,593
pykF
Position (kb)
1006
1007
1008 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1005.645 kb on + strand, within sufS at 1005.666 kb on - strand, within sufS at 1005.668 kb on + strand, within sufS at 1005.829 kb on - strand, within sufS at 1005.921 kb on + strand at 1005.921 kb on + strand at 1005.922 kb on - strand at 1005.922 kb on - strand at 1005.923 kb on + strand at 1005.924 kb on - strand at 1005.968 kb on + strand at 1006.002 kb on - strand at 1006.071 kb on + strand, within sufE at 1006.071 kb on + strand, within sufE at 1006.091 kb on - strand, within sufE at 1006.416 kb on + strand at 1006.422 kb on - strand at 1006.514 kb on - strand at 1006.690 kb on + strand, within ldtE at 1006.691 kb on - strand, within ldtE at 1006.773 kb on + strand, within ldtE at 1006.812 kb on - strand, within ldtE at 1006.833 kb on - strand, within ldtE at 1006.892 kb on - strand, within ldtE at 1006.892 kb on - strand, within ldtE at 1006.931 kb on + strand, within ldtE at 1006.935 kb on + strand, within ldtE at 1006.935 kb on + strand, within ldtE at 1006.936 kb on - strand, within ldtE at 1007.318 kb on + strand, within ldtE at 1007.319 kb on - strand, within ldtE at 1007.319 kb on - strand, within ldtE at 1007.331 kb on - strand, within ldtE at 1007.443 kb on + strand, within ldtE at 1007.514 kb on - strand at 1007.551 kb on - strand at 1007.570 kb on - strand at 1007.580 kb on - strand at 1007.754 kb on - strand, within lpp at 1007.826 kb on - strand, within lpp at 1007.826 kb on - strand, within lpp at 1007.875 kb on - strand at 1007.875 kb on - strand at 1007.894 kb on + strand at 1007.906 kb on - strand at 1008.077 kb on - strand at 1008.077 kb on - strand at 1008.211 kb on - strand at 1008.248 kb on - strand at 1008.248 kb on - strand at 1008.319 kb on - strand at 1008.319 kb on - strand at 1008.425 kb on + strand, within pykF
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,005,645 + sufS HEPCGN_05655 0.72 +0.0 1,005,666 - sufS HEPCGN_05655 0.73 -0.5 1,005,668 + sufS HEPCGN_05655 0.74 -0.3 1,005,829 - sufS HEPCGN_05655 0.87 -0.3 1,005,921 + +0.2 1,005,921 + -0.4 1,005,922 - -0.7 1,005,922 - +1.1 1,005,923 + -0.8 1,005,924 - -1.4 1,005,968 + -0.0 1,006,002 - +0.1 1,006,071 + sufE HEPCGN_05660 0.17 +0.3 1,006,071 + sufE HEPCGN_05660 0.17 -3.7 1,006,091 - sufE HEPCGN_05660 0.21 +0.7 1,006,416 + +0.7 1,006,422 - -0.1 1,006,514 - +0.6 1,006,690 + ldtE HEPCGN_05665 0.12 +0.7 1,006,691 - ldtE HEPCGN_05665 0.12 -0.5 1,006,773 + ldtE HEPCGN_05665 0.20 -1.6 1,006,812 - ldtE HEPCGN_05665 0.24 -0.0 1,006,833 - ldtE HEPCGN_05665 0.26 +0.1 1,006,892 - ldtE HEPCGN_05665 0.32 -1.4 1,006,892 - ldtE HEPCGN_05665 0.32 +0.4 1,006,931 + ldtE HEPCGN_05665 0.36 -1.6 1,006,935 + ldtE HEPCGN_05665 0.37 -0.6 1,006,935 + ldtE HEPCGN_05665 0.37 +0.1 1,006,936 - ldtE HEPCGN_05665 0.37 -0.9 1,007,318 + ldtE HEPCGN_05665 0.75 -0.8 1,007,319 - ldtE HEPCGN_05665 0.75 +0.3 1,007,319 - ldtE HEPCGN_05665 0.75 +0.0 1,007,331 - ldtE HEPCGN_05665 0.76 +0.2 1,007,443 + ldtE HEPCGN_05665 0.87 +1.7 1,007,514 - +1.7 1,007,551 - -0.4 1,007,570 - +3.4 1,007,580 - +0.1 1,007,754 - lpp HEPCGN_05670 0.51 +0.8 1,007,826 - lpp HEPCGN_05670 0.81 +0.0 1,007,826 - lpp HEPCGN_05670 0.81 -0.3 1,007,875 - -0.0 1,007,875 - +0.6 1,007,894 + +0.5 1,007,906 - -0.2 1,008,077 - +1.5 1,008,077 - -0.1 1,008,211 - -2.4 1,008,248 - +0.2 1,008,248 - +0.4 1,008,319 - -0.0 1,008,319 - +0.4 1,008,425 + pykF HEPCGN_05675 0.17 +0.9
Or see this region's nucleotide sequence