Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydjY and ydjX are separated by 4 nucleotides ydjX and xthA are separated by 166 nucleotides xthA and astC are separated by 445 nucleotides
HEPCGN_05255: ydjY - Uncharacterized protein YdjY, at 927,018 to 927,695
ydjY
HEPCGN_05260: ydjX - TVP38/TMEM64 family membrane protein YdjX, at 927,700 to 928,410
ydjX
HEPCGN_05265: xthA - exodeoxyribonuclease III, at 928,577 to 929,383
xthA
HEPCGN_05270: astC - succinylornithine/acetylornithine transaminase, at 929,829 to 931,049
astC
Position (kb) 928
929
930 Strain fitness (log2 ratio) -2
-1
0
1
2 at 927.701 kb on + strand at 927.723 kb on + strand at 927.723 kb on + strand at 927.723 kb on + strand at 927.830 kb on - strand, within ydjX at 927.885 kb on - strand, within ydjX at 927.976 kb on - strand, within ydjX at 928.056 kb on + strand, within ydjX at 928.079 kb on + strand, within ydjX at 928.090 kb on + strand, within ydjX at 928.153 kb on + strand, within ydjX at 928.153 kb on + strand, within ydjX at 928.162 kb on + strand, within ydjX at 928.210 kb on + strand, within ydjX at 928.257 kb on + strand, within ydjX at 928.258 kb on - strand, within ydjX at 928.258 kb on - strand, within ydjX at 928.260 kb on - strand, within ydjX at 928.267 kb on - strand, within ydjX at 928.269 kb on - strand, within ydjX at 928.477 kb on + strand at 928.477 kb on + strand at 928.508 kb on - strand at 928.537 kb on + strand at 928.538 kb on - strand at 928.880 kb on - strand, within xthA at 928.998 kb on + strand, within xthA at 929.059 kb on - strand, within xthA at 929.110 kb on + strand, within xthA at 929.111 kb on - strand, within xthA at 929.263 kb on + strand, within xthA at 929.264 kb on - strand, within xthA at 929.366 kb on - strand at 929.424 kb on - strand at 929.456 kb on + strand at 929.668 kb on - strand at 929.675 kb on + strand at 929.829 kb on - strand at 929.843 kb on + strand at 929.873 kb on - strand at 929.897 kb on + strand at 929.897 kb on + strand at 929.898 kb on - strand at 929.898 kb on - strand at 929.898 kb on - strand at 929.899 kb on + strand at 929.950 kb on - strand at 929.951 kb on + strand, within astC at 930.135 kb on - strand, within astC at 930.289 kb on - strand, within astC at 930.336 kb on + strand, within astC at 930.337 kb on - strand, within astC at 930.339 kb on - strand, within astC at 930.348 kb on - strand, within astC at 930.377 kb on + strand, within astC at 930.378 kb on - strand, within astC at 930.378 kb on - strand, within astC
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 927,701 + +0.5 927,723 + -0.3 927,723 + +0.6 927,723 + -0.4 927,830 - ydjX HEPCGN_05260 0.18 -0.6 927,885 - ydjX HEPCGN_05260 0.26 +0.7 927,976 - ydjX HEPCGN_05260 0.39 +1.1 928,056 + ydjX HEPCGN_05260 0.50 -0.0 928,079 + ydjX HEPCGN_05260 0.53 +0.0 928,090 + ydjX HEPCGN_05260 0.55 +0.2 928,153 + ydjX HEPCGN_05260 0.64 -0.3 928,153 + ydjX HEPCGN_05260 0.64 +0.1 928,162 + ydjX HEPCGN_05260 0.65 -0.3 928,210 + ydjX HEPCGN_05260 0.72 +1.4 928,257 + ydjX HEPCGN_05260 0.78 -2.4 928,258 - ydjX HEPCGN_05260 0.78 +1.5 928,258 - ydjX HEPCGN_05260 0.78 -1.1 928,260 - ydjX HEPCGN_05260 0.79 +0.1 928,267 - ydjX HEPCGN_05260 0.80 +1.0 928,269 - ydjX HEPCGN_05260 0.80 -0.1 928,477 + +0.9 928,477 + +0.2 928,508 - +1.6 928,537 + +0.4 928,538 - +0.7 928,880 - xthA HEPCGN_05265 0.38 -1.1 928,998 + xthA HEPCGN_05265 0.52 -0.2 929,059 - xthA HEPCGN_05265 0.60 -2.1 929,110 + xthA HEPCGN_05265 0.66 +0.6 929,111 - xthA HEPCGN_05265 0.66 -2.6 929,263 + xthA HEPCGN_05265 0.85 -0.1 929,264 - xthA HEPCGN_05265 0.85 -0.1 929,366 - -0.2 929,424 - -1.1 929,456 + +0.3 929,668 - +0.5 929,675 + +0.4 929,829 - +0.9 929,843 + -1.0 929,873 - -0.6 929,897 + +0.6 929,897 + +1.0 929,898 - +0.8 929,898 - +1.8 929,898 - -0.4 929,899 + -0.0 929,950 - +0.8 929,951 + astC HEPCGN_05270 0.10 +0.2 930,135 - astC HEPCGN_05270 0.25 -0.5 930,289 - astC HEPCGN_05270 0.38 -0.3 930,336 + astC HEPCGN_05270 0.42 -0.0 930,337 - astC HEPCGN_05270 0.42 +1.2 930,339 - astC HEPCGN_05270 0.42 +0.4 930,348 - astC HEPCGN_05270 0.43 +0.3 930,377 + astC HEPCGN_05270 0.45 +0.6 930,378 - astC HEPCGN_05270 0.45 -1.1 930,378 - astC HEPCGN_05270 0.45 -0.1
Or see this region's nucleotide sequence