Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yobB and holE are separated by 101 nucleotides holE and yobA are separated by 138 nucleotides yobA and copD are separated by 3 nucleotides copD and copD are separated by 12 nucleotides
HEPCGN_04790: yobB - Uncharacterized protein YobB, at 836,709 to 837,365
yobB
HEPCGN_04795: holE - DNA polymerase III subunit theta, at 837,467 to 837,697
holE
HEPCGN_04800: yobA - CopC domain-containing protein YobA, at 837,836 to 838,210
yobA
HEPCGN_04805: copD - copper homeostasis membrane protein CopD, at 838,214 to 839,086
copD
HEPCGN_04810: copD - copper homeostasis membrane protein CopD, at 839,099 to 839,440
copD
Position (kb) 837
838
839 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 836.966 kb on + strand, within yobB at 836.966 kb on + strand, within yobB at 836.966 kb on + strand, within yobB at 836.966 kb on + strand, within yobB at 837.041 kb on - strand, within yobB at 837.056 kb on + strand, within yobB at 837.057 kb on - strand, within yobB at 837.103 kb on + strand, within yobB at 837.104 kb on - strand, within yobB at 837.104 kb on - strand, within yobB at 837.169 kb on - strand, within yobB at 837.172 kb on - strand, within yobB at 837.300 kb on + strand at 837.334 kb on + strand at 837.335 kb on - strand at 837.335 kb on - strand at 837.366 kb on - strand at 837.460 kb on - strand at 837.460 kb on - strand at 837.602 kb on - strand, within holE at 837.602 kb on - strand, within holE at 837.606 kb on + strand, within holE at 837.606 kb on + strand, within holE at 837.606 kb on + strand, within holE at 837.616 kb on + strand, within holE at 837.616 kb on + strand, within holE at 837.617 kb on - strand, within holE at 837.814 kb on - strand at 837.832 kb on + strand at 837.833 kb on - strand at 837.935 kb on + strand, within yobA at 837.984 kb on + strand, within yobA at 838.137 kb on - strand, within yobA at 838.137 kb on - strand, within yobA at 838.174 kb on + strand at 838.289 kb on + strand at 838.289 kb on + strand at 838.540 kb on + strand, within copD at 838.583 kb on - strand, within copD at 838.661 kb on - strand, within copD at 838.661 kb on - strand, within copD at 838.708 kb on - strand, within copD at 838.727 kb on - strand, within copD at 838.921 kb on + strand, within copD at 838.921 kb on + strand, within copD at 838.921 kb on + strand, within copD at 838.949 kb on + strand, within copD at 839.032 kb on - strand at 839.093 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 836,966 + yobB HEPCGN_04790 0.39 -0.1 836,966 + yobB HEPCGN_04790 0.39 +0.9 836,966 + yobB HEPCGN_04790 0.39 +0.5 836,966 + yobB HEPCGN_04790 0.39 +0.7 837,041 - yobB HEPCGN_04790 0.51 +0.2 837,056 + yobB HEPCGN_04790 0.53 -0.1 837,057 - yobB HEPCGN_04790 0.53 +0.8 837,103 + yobB HEPCGN_04790 0.60 +0.5 837,104 - yobB HEPCGN_04790 0.60 -0.3 837,104 - yobB HEPCGN_04790 0.60 +0.1 837,169 - yobB HEPCGN_04790 0.70 +0.3 837,172 - yobB HEPCGN_04790 0.70 +0.2 837,300 + -0.0 837,334 + -0.0 837,335 - +0.1 837,335 - -0.5 837,366 - -0.8 837,460 - +1.0 837,460 - -2.0 837,602 - holE HEPCGN_04795 0.58 +0.3 837,602 - holE HEPCGN_04795 0.58 +0.1 837,606 + holE HEPCGN_04795 0.60 +0.9 837,606 + holE HEPCGN_04795 0.60 +0.2 837,606 + holE HEPCGN_04795 0.60 +0.7 837,616 + holE HEPCGN_04795 0.65 +0.4 837,616 + holE HEPCGN_04795 0.65 -0.0 837,617 - holE HEPCGN_04795 0.65 +0.5 837,814 - +0.4 837,832 + +0.1 837,833 - -0.1 837,935 + yobA HEPCGN_04800 0.26 +0.6 837,984 + yobA HEPCGN_04800 0.39 -0.2 838,137 - yobA HEPCGN_04800 0.80 -0.2 838,137 - yobA HEPCGN_04800 0.80 -1.0 838,174 + -0.0 838,289 + +0.4 838,289 + +0.5 838,540 + copD HEPCGN_04805 0.37 +0.6 838,583 - copD HEPCGN_04805 0.42 -0.3 838,661 - copD HEPCGN_04805 0.51 +0.6 838,661 - copD HEPCGN_04805 0.51 -1.8 838,708 - copD HEPCGN_04805 0.57 +0.1 838,727 - copD HEPCGN_04805 0.59 +0.0 838,921 + copD HEPCGN_04805 0.81 -0.3 838,921 + copD HEPCGN_04805 0.81 -4.6 838,921 + copD HEPCGN_04805 0.81 +0.7 838,949 + copD HEPCGN_04805 0.84 +0.7 839,032 - -0.2 839,093 - +0.1
Or see this region's nucleotide sequence