Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt moaB and moaC are separated by 2 nucleotides moaC and moaD overlap by 8 nucleotides moaD and moaE are separated by 1 nucleotides moaE and ybhL are separated by 136 nucleotides
HEPCGN_01675: moaB - molybdenum cofactor biosynthesis protein B, at 259,750 to 260,262
moaB
HEPCGN_01680: moaC - cyclic pyranopterin monophosphate synthase MoaC, at 260,265 to 260,750
moaC
HEPCGN_01685: moaD - molybdopterin synthase sulfur carrier subunit, at 260,743 to 260,988
moaD
HEPCGN_01690: moaE - molybdopterin synthase catalytic subunit MoaE, at 260,990 to 261,442
moaE
HEPCGN_01695: ybhL - Inner membrane protein YbhL, at 261,579 to 262,283
ybhL
Position (kb) 260
261 Strain fitness (log2 ratio) -2
-1
0
1
2 at 259.786 kb on - strand at 259.791 kb on + strand at 259.792 kb on - strand at 259.792 kb on - strand at 259.830 kb on + strand, within moaB at 259.918 kb on - strand, within moaB at 259.932 kb on - strand, within moaB at 259.959 kb on - strand, within moaB at 259.959 kb on - strand, within moaB at 259.959 kb on - strand, within moaB at 259.959 kb on - strand, within moaB at 260.260 kb on + strand at 260.260 kb on + strand at 260.260 kb on + strand at 260.261 kb on - strand at 260.261 kb on - strand at 260.264 kb on + strand at 260.425 kb on + strand, within moaC at 260.426 kb on - strand, within moaC at 260.442 kb on - strand, within moaC at 260.555 kb on - strand, within moaC at 260.558 kb on + strand, within moaC at 260.558 kb on + strand, within moaC at 260.559 kb on - strand, within moaC at 260.559 kb on - strand, within moaC at 260.559 kb on - strand, within moaC at 260.560 kb on + strand, within moaC at 260.561 kb on - strand, within moaC at 260.569 kb on + strand, within moaC at 260.570 kb on - strand, within moaC at 260.643 kb on + strand, within moaC at 260.643 kb on + strand, within moaC at 260.677 kb on + strand, within moaC at 260.677 kb on + strand, within moaC at 260.678 kb on - strand, within moaC at 260.729 kb on + strand at 260.730 kb on - strand at 260.748 kb on + strand at 260.749 kb on - strand at 260.957 kb on + strand, within moaD at 260.958 kb on - strand, within moaD at 261.112 kb on + strand, within moaE at 261.329 kb on - strand, within moaE at 261.338 kb on + strand, within moaE at 261.495 kb on + strand at 261.495 kb on + strand at 261.495 kb on + strand at 261.496 kb on - strand at 261.542 kb on - strand at 261.547 kb on + strand at 261.559 kb on + strand at 261.560 kb on - strand at 261.561 kb on + strand at 261.562 kb on - strand at 261.562 kb on - strand at 261.611 kb on - strand at 261.636 kb on + strand at 261.636 kb on + strand at 261.637 kb on - strand at 261.637 kb on - strand at 261.651 kb on + strand, within ybhL at 261.667 kb on + strand, within ybhL at 261.667 kb on + strand, within ybhL at 261.668 kb on - strand, within ybhL at 261.707 kb on + strand, within ybhL at 261.722 kb on + strand, within ybhL at 261.730 kb on + strand, within ybhL at 261.730 kb on + strand, within ybhL at 261.731 kb on - strand, within ybhL at 261.731 kb on - strand, within ybhL at 261.733 kb on + strand, within ybhL at 261.766 kb on + strand, within ybhL at 261.767 kb on - strand, within ybhL at 261.767 kb on - strand, within ybhL at 261.769 kb on + strand, within ybhL at 261.779 kb on - strand, within ybhL at 261.789 kb on - strand, within ybhL at 261.796 kb on + strand, within ybhL at 261.830 kb on - strand, within ybhL at 261.900 kb on - strand, within ybhL at 261.911 kb on + strand, within ybhL
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 259,786 - +0.7 259,791 + +0.2 259,792 - -1.6 259,792 - +0.4 259,830 + moaB HEPCGN_01675 0.16 -0.1 259,918 - moaB HEPCGN_01675 0.33 -0.5 259,932 - moaB HEPCGN_01675 0.35 -0.1 259,959 - moaB HEPCGN_01675 0.41 +0.2 259,959 - moaB HEPCGN_01675 0.41 +1.3 259,959 - moaB HEPCGN_01675 0.41 -0.2 259,959 - moaB HEPCGN_01675 0.41 +0.7 260,260 + -0.7 260,260 + -1.3 260,260 + +0.6 260,261 - -0.8 260,261 - -1.4 260,264 + -0.9 260,425 + moaC HEPCGN_01680 0.33 -0.5 260,426 - moaC HEPCGN_01680 0.33 -1.2 260,442 - moaC HEPCGN_01680 0.36 +0.4 260,555 - moaC HEPCGN_01680 0.60 -2.1 260,558 + moaC HEPCGN_01680 0.60 -0.1 260,558 + moaC HEPCGN_01680 0.60 -1.0 260,559 - moaC HEPCGN_01680 0.60 -0.2 260,559 - moaC HEPCGN_01680 0.60 +0.1 260,559 - moaC HEPCGN_01680 0.60 -1.2 260,560 + moaC HEPCGN_01680 0.61 +1.4 260,561 - moaC HEPCGN_01680 0.61 +0.5 260,569 + moaC HEPCGN_01680 0.63 +0.0 260,570 - moaC HEPCGN_01680 0.63 -2.7 260,643 + moaC HEPCGN_01680 0.78 -0.2 260,643 + moaC HEPCGN_01680 0.78 +0.4 260,677 + moaC HEPCGN_01680 0.85 -0.3 260,677 + moaC HEPCGN_01680 0.85 +0.3 260,678 - moaC HEPCGN_01680 0.85 -0.1 260,729 + -0.6 260,730 - +0.5 260,748 + +1.4 260,749 - +0.0 260,957 + moaD HEPCGN_01685 0.87 -0.7 260,958 - moaD HEPCGN_01685 0.87 -0.9 261,112 + moaE HEPCGN_01690 0.27 +1.5 261,329 - moaE HEPCGN_01690 0.75 -0.5 261,338 + moaE HEPCGN_01690 0.77 -0.1 261,495 + +1.8 261,495 + +0.9 261,495 + +0.5 261,496 - -0.5 261,542 - +0.4 261,547 + -0.2 261,559 + +0.5 261,560 - -0.2 261,561 + -1.0 261,562 - -0.4 261,562 - -0.2 261,611 - -0.4 261,636 + -1.8 261,636 + -0.0 261,637 - -0.4 261,637 - +0.9 261,651 + ybhL HEPCGN_01695 0.10 +0.1 261,667 + ybhL HEPCGN_01695 0.12 -1.6 261,667 + ybhL HEPCGN_01695 0.12 +0.7 261,668 - ybhL HEPCGN_01695 0.13 -1.6 261,707 + ybhL HEPCGN_01695 0.18 -0.1 261,722 + ybhL HEPCGN_01695 0.20 +0.2 261,730 + ybhL HEPCGN_01695 0.21 +0.9 261,730 + ybhL HEPCGN_01695 0.21 +0.1 261,731 - ybhL HEPCGN_01695 0.22 +0.5 261,731 - ybhL HEPCGN_01695 0.22 +1.2 261,733 + ybhL HEPCGN_01695 0.22 -1.1 261,766 + ybhL HEPCGN_01695 0.27 +0.3 261,767 - ybhL HEPCGN_01695 0.27 -1.0 261,767 - ybhL HEPCGN_01695 0.27 -0.0 261,769 + ybhL HEPCGN_01695 0.27 +0.4 261,779 - ybhL HEPCGN_01695 0.28 -0.1 261,789 - ybhL HEPCGN_01695 0.30 -0.6 261,796 + ybhL HEPCGN_01695 0.31 -0.7 261,830 - ybhL HEPCGN_01695 0.36 +0.2 261,900 - ybhL HEPCGN_01695 0.46 -0.3 261,911 + ybhL HEPCGN_01695 0.47 -0.6
Or see this region's nucleotide sequence