Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt chiP and chiQ are separated by 49 nucleotides chiQ and fur are separated by 83 nucleotides fur and uof overlap by 8 nucleotides uof and fldA are separated by 209 nucleotides
HEPCGN_01085: chiP - chitoporin, at 142,912 to 144,318
chiP
HEPCGN_01090: chiQ - Uncharacterized lipoprotein ChiQ, at 144,368 to 144,694
chiQ
HEPCGN_01095: fur - ferric iron uptake transcriptional regulator, at 144,778 to 145,224
fur
HEPCGN_01100: uof - fur leader peptide, at 145,217 to 145,303
uof
HEPCGN_01105: fldA - flavodoxin FldA, at 145,513 to 146,043
fldA
Position (kb) 144
145 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 143.419 kb on - strand, within chiP at 143.422 kb on + strand, within chiP at 143.423 kb on - strand, within chiP at 143.423 kb on - strand, within chiP at 143.485 kb on + strand, within chiP at 143.504 kb on - strand, within chiP at 143.593 kb on + strand, within chiP at 143.647 kb on + strand, within chiP at 143.659 kb on + strand, within chiP at 143.712 kb on + strand, within chiP at 143.770 kb on + strand, within chiP at 143.879 kb on - strand, within chiP at 143.893 kb on - strand, within chiP at 143.893 kb on - strand, within chiP at 143.905 kb on + strand, within chiP at 143.906 kb on - strand, within chiP at 143.932 kb on - strand, within chiP at 143.998 kb on + strand, within chiP at 143.998 kb on + strand, within chiP at 143.998 kb on + strand, within chiP at 143.998 kb on + strand, within chiP at 144.053 kb on - strand, within chiP at 144.100 kb on + strand, within chiP at 144.120 kb on + strand, within chiP at 144.155 kb on - strand, within chiP at 144.294 kb on - strand at 144.411 kb on + strand, within chiQ at 144.464 kb on + strand, within chiQ at 144.465 kb on - strand, within chiQ at 144.475 kb on + strand, within chiQ at 144.597 kb on - strand, within chiQ at 144.682 kb on + strand at 144.693 kb on - strand at 144.693 kb on - strand at 144.706 kb on - strand at 144.779 kb on + strand at 145.061 kb on - strand, within fur at 145.107 kb on - strand, within fur at 145.242 kb on - strand, within uof at 145.307 kb on - strand at 145.358 kb on + strand at 145.377 kb on - strand at 145.377 kb on - strand at 145.382 kb on + strand at 145.383 kb on - strand at 145.383 kb on - strand at 145.383 kb on - strand at 145.488 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 143,419 - chiP HEPCGN_01085 0.36 +0.3 143,422 + chiP HEPCGN_01085 0.36 +0.3 143,423 - chiP HEPCGN_01085 0.36 -1.3 143,423 - chiP HEPCGN_01085 0.36 -0.1 143,485 + chiP HEPCGN_01085 0.41 -0.9 143,504 - chiP HEPCGN_01085 0.42 -0.4 143,593 + chiP HEPCGN_01085 0.48 -0.6 143,647 + chiP HEPCGN_01085 0.52 -0.1 143,659 + chiP HEPCGN_01085 0.53 +0.9 143,712 + chiP HEPCGN_01085 0.57 +0.0 143,770 + chiP HEPCGN_01085 0.61 +0.5 143,879 - chiP HEPCGN_01085 0.69 +0.3 143,893 - chiP HEPCGN_01085 0.70 -0.6 143,893 - chiP HEPCGN_01085 0.70 +0.2 143,905 + chiP HEPCGN_01085 0.71 +0.6 143,906 - chiP HEPCGN_01085 0.71 -0.1 143,932 - chiP HEPCGN_01085 0.72 -0.2 143,998 + chiP HEPCGN_01085 0.77 +0.3 143,998 + chiP HEPCGN_01085 0.77 +0.6 143,998 + chiP HEPCGN_01085 0.77 -0.2 143,998 + chiP HEPCGN_01085 0.77 -1.2 144,053 - chiP HEPCGN_01085 0.81 +0.5 144,100 + chiP HEPCGN_01085 0.84 +1.2 144,120 + chiP HEPCGN_01085 0.86 +0.5 144,155 - chiP HEPCGN_01085 0.88 +1.2 144,294 - +0.8 144,411 + chiQ HEPCGN_01090 0.13 +0.8 144,464 + chiQ HEPCGN_01090 0.29 -0.1 144,465 - chiQ HEPCGN_01090 0.30 +0.2 144,475 + chiQ HEPCGN_01090 0.33 +0.8 144,597 - chiQ HEPCGN_01090 0.70 +0.5 144,682 + +0.1 144,693 - -0.7 144,693 - -0.9 144,706 - +0.7 144,779 + +0.9 145,061 - fur HEPCGN_01095 0.63 -0.5 145,107 - fur HEPCGN_01095 0.74 -0.8 145,242 - uof HEPCGN_01100 0.29 -1.4 145,307 - -3.4 145,358 + -0.5 145,377 - +0.6 145,377 - +0.2 145,382 + +0.1 145,383 - +0.2 145,383 - -0.2 145,383 - +0.2 145,488 - +0.6
Or see this region's nucleotide sequence