Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt entS and fepB are separated by 3 nucleotides fepB and entC are separated by 188 nucleotides
HEPCGN_00695: entS - enterobactin exporter, iron-regulated, at 67,611 to 68,861
entS
HEPCGN_00700: fepB - ferrienterobactin ABC transporter periplasmic binding protein, at 68,865 to 69,821
fepB
HEPCGN_00705: entC - isochorismate synthase 1, at 70,010 to 71,185
entC
Position (kb) 68
69
70 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 67.951 kb on - strand, within entS at 67.955 kb on - strand, within entS at 67.955 kb on - strand, within entS at 68.192 kb on - strand, within entS at 68.315 kb on - strand, within entS at 68.459 kb on + strand, within entS at 68.460 kb on - strand, within entS at 68.460 kb on - strand, within entS at 68.460 kb on - strand, within entS at 68.549 kb on - strand, within entS at 68.591 kb on + strand, within entS at 68.608 kb on - strand, within entS at 68.608 kb on - strand, within entS at 68.609 kb on + strand, within entS at 68.609 kb on + strand, within entS at 68.609 kb on + strand, within entS at 68.859 kb on + strand at 68.866 kb on + strand at 68.866 kb on + strand at 68.880 kb on - strand at 68.880 kb on - strand at 68.908 kb on + strand at 68.909 kb on - strand at 68.912 kb on - strand at 69.225 kb on - strand, within fepB at 69.227 kb on - strand, within fepB at 69.463 kb on - strand, within fepB at 69.517 kb on + strand, within fepB at 69.517 kb on + strand, within fepB at 69.518 kb on - strand, within fepB at 69.518 kb on - strand, within fepB at 69.677 kb on + strand, within fepB at 69.702 kb on - strand, within fepB at 69.759 kb on + strand at 69.784 kb on - strand at 69.787 kb on - strand at 69.841 kb on - strand at 69.844 kb on - strand at 69.910 kb on - strand at 69.926 kb on + strand at 69.926 kb on + strand at 70.010 kb on - strand at 70.015 kb on + strand at 70.015 kb on + strand at 70.015 kb on + strand at 70.015 kb on + strand at 70.015 kb on + strand at 70.016 kb on - strand at 70.016 kb on - strand at 70.016 kb on - strand at 70.016 kb on - strand at 70.078 kb on + strand at 70.079 kb on - strand at 70.099 kb on + strand at 70.277 kb on - strand, within entC at 70.426 kb on + strand, within entC at 70.613 kb on - strand, within entC at 70.780 kb on + strand, within entC at 70.788 kb on + strand, within entC
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 67,951 - entS HEPCGN_00695 0.27 +0.6 67,955 - entS HEPCGN_00695 0.27 -2.2 67,955 - entS HEPCGN_00695 0.27 -0.2 68,192 - entS HEPCGN_00695 0.46 -1.0 68,315 - entS HEPCGN_00695 0.56 -0.7 68,459 + entS HEPCGN_00695 0.68 -1.1 68,460 - entS HEPCGN_00695 0.68 -0.5 68,460 - entS HEPCGN_00695 0.68 -0.2 68,460 - entS HEPCGN_00695 0.68 -0.3 68,549 - entS HEPCGN_00695 0.75 -0.2 68,591 + entS HEPCGN_00695 0.78 +0.0 68,608 - entS HEPCGN_00695 0.80 +0.3 68,608 - entS HEPCGN_00695 0.80 +0.6 68,609 + entS HEPCGN_00695 0.80 -1.1 68,609 + entS HEPCGN_00695 0.80 +0.1 68,609 + entS HEPCGN_00695 0.80 -0.6 68,859 + +0.2 68,866 + -0.2 68,866 + +1.0 68,880 - -0.7 68,880 - +0.6 68,908 + -1.8 68,909 - -0.3 68,912 - +0.3 69,225 - fepB HEPCGN_00700 0.38 -0.3 69,227 - fepB HEPCGN_00700 0.38 +0.5 69,463 - fepB HEPCGN_00700 0.62 -1.1 69,517 + fepB HEPCGN_00700 0.68 -2.9 69,517 + fepB HEPCGN_00700 0.68 -1.5 69,518 - fepB HEPCGN_00700 0.68 -1.3 69,518 - fepB HEPCGN_00700 0.68 -1.5 69,677 + fepB HEPCGN_00700 0.85 +0.8 69,702 - fepB HEPCGN_00700 0.87 -0.6 69,759 + -0.8 69,784 - -1.0 69,787 - -0.6 69,841 - -0.5 69,844 - -0.5 69,910 - +0.7 69,926 + +0.3 69,926 + -0.7 70,010 - -0.6 70,015 + +0.1 70,015 + -0.4 70,015 + -0.4 70,015 + +1.7 70,015 + -0.3 70,016 - +1.0 70,016 - -0.3 70,016 - -1.9 70,016 - +1.6 70,078 + +0.5 70,079 - -0.2 70,099 + -0.3 70,277 - entC HEPCGN_00705 0.23 +0.0 70,426 + entC HEPCGN_00705 0.35 -0.6 70,613 - entC HEPCGN_00705 0.51 -0.1 70,780 + entC HEPCGN_00705 0.65 +0.3 70,788 + entC HEPCGN_00705 0.66 +0.6
Or see this region's nucleotide sequence