Experiment: L-Rhamnose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisF and hisA overlap by 19 nucleotides hisA and hisH overlap by 1 nucleotides hisH and hisB overlap by 1 nucleotides
NOLOHH_15805: hisF - Imidazole glycerol phosphate synthase subunit HisF, at 3,116,570 to 3,117,346
hisF
NOLOHH_15810: hisA - 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)me thylideneamino]imidazole-4-carboxamide isomerase, at 3,117,328 to 3,118,065
hisA
NOLOHH_15815: hisH - imidazole glycerol phosphate synthase subunit HisH, at 3,118,065 to 3,118,655
hisH
NOLOHH_15820: hisB - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 3,118,655 to 3,119,722
hisB
Position (kb)
3118
3119 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 3117.165 kb on - strand, within hisF at 3117.327 kb on - strand at 3117.330 kb on - strand at 3117.398 kb on - strand at 3117.398 kb on - strand at 3117.398 kb on - strand at 3117.401 kb on - strand at 3117.507 kb on - strand, within hisA at 3117.507 kb on - strand, within hisA at 3117.507 kb on - strand, within hisA at 3117.509 kb on - strand, within hisA at 3117.695 kb on - strand, within hisA at 3117.871 kb on - strand, within hisA at 3117.871 kb on - strand, within hisA at 3117.962 kb on - strand, within hisA at 3117.962 kb on - strand, within hisA at 3117.962 kb on - strand, within hisA at 3118.139 kb on - strand, within hisH at 3118.184 kb on + strand, within hisH at 3118.184 kb on + strand, within hisH at 3118.185 kb on - strand, within hisH at 3118.185 kb on - strand, within hisH at 3118.185 kb on - strand, within hisH at 3118.237 kb on + strand, within hisH at 3118.766 kb on - strand, within hisB at 3118.880 kb on - strand, within hisB at 3118.880 kb on - strand, within hisB at 3118.880 kb on - strand, within hisB at 3118.907 kb on - strand, within hisB at 3119.050 kb on - strand, within hisB at 3119.141 kb on - strand, within hisB at 3119.346 kb on + strand, within hisB at 3119.346 kb on + strand, within hisB at 3119.347 kb on - strand, within hisB
Per-strain Table
Position Strand Gene LocusTag Fraction L-Rhamnose remove 3,117,165 - hisF NOLOHH_15805 0.77 -3.3 3,117,327 - -2.7 3,117,330 - -2.9 3,117,398 - -2.9 3,117,398 - -2.8 3,117,398 - -4.3 3,117,401 - -3.7 3,117,507 - hisA NOLOHH_15810 0.24 -4.0 3,117,507 - hisA NOLOHH_15810 0.24 -1.2 3,117,507 - hisA NOLOHH_15810 0.24 -4.2 3,117,509 - hisA NOLOHH_15810 0.25 -1.0 3,117,695 - hisA NOLOHH_15810 0.50 -3.7 3,117,871 - hisA NOLOHH_15810 0.74 -4.9 3,117,871 - hisA NOLOHH_15810 0.74 -5.0 3,117,962 - hisA NOLOHH_15810 0.86 -3.7 3,117,962 - hisA NOLOHH_15810 0.86 -0.4 3,117,962 - hisA NOLOHH_15810 0.86 -4.1 3,118,139 - hisH NOLOHH_15815 0.13 -5.3 3,118,184 + hisH NOLOHH_15815 0.20 -0.7 3,118,184 + hisH NOLOHH_15815 0.20 -3.9 3,118,185 - hisH NOLOHH_15815 0.20 -3.7 3,118,185 - hisH NOLOHH_15815 0.20 -1.7 3,118,185 - hisH NOLOHH_15815 0.20 -3.7 3,118,237 + hisH NOLOHH_15815 0.29 -2.2 3,118,766 - hisB NOLOHH_15820 0.10 -2.4 3,118,880 - hisB NOLOHH_15820 0.21 -3.5 3,118,880 - hisB NOLOHH_15820 0.21 -3.4 3,118,880 - hisB NOLOHH_15820 0.21 -2.1 3,118,907 - hisB NOLOHH_15820 0.24 -5.9 3,119,050 - hisB NOLOHH_15820 0.37 -1.4 3,119,141 - hisB NOLOHH_15820 0.46 -4.7 3,119,346 + hisB NOLOHH_15820 0.65 -3.3 3,119,346 + hisB NOLOHH_15820 0.65 -1.3 3,119,347 - hisB NOLOHH_15820 0.65 -4.0
Or see this region's nucleotide sequence