Experiment: L-Rhamnose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt leuO and leuL are separated by 659 nucleotides leuL and NOLOHH_03060 are separated by 92 nucleotides NOLOHH_03060 and leuA overlap by 1 nucleotides
NOLOHH_03050: leuO - transcriptional regulator LeuO, at 523,918 to 524,862
leuO
NOLOHH_03055: leuL - leu operon leader peptide, at 525,522 to 525,608
leuL
NOLOHH_03060: NOLOHH_03060 - 2-isopropylmalate synthase, at 525,701 to 527,272
_03060
NOLOHH_03065: leuA - 2-isopropylmalate synthase, at 527,272 to 528,363
leuA
Position (kb) 525
526
527
528 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 524.721 kb on + strand, within leuO at 524.722 kb on - strand, within leuO at 524.785 kb on + strand at 524.785 kb on + strand at 524.785 kb on + strand at 524.786 kb on - strand at 524.786 kb on - strand at 524.806 kb on + strand at 524.807 kb on - strand at 524.807 kb on - strand at 524.807 kb on - strand at 524.884 kb on + strand at 524.884 kb on + strand at 524.885 kb on - strand at 524.937 kb on + strand at 524.937 kb on + strand at 524.938 kb on - strand at 525.052 kb on - strand at 525.097 kb on - strand at 525.117 kb on + strand at 525.196 kb on - strand at 525.237 kb on + strand at 525.329 kb on + strand at 525.359 kb on - strand at 525.402 kb on + strand at 525.488 kb on + strand at 525.489 kb on - strand at 525.496 kb on + strand at 525.520 kb on + strand at 525.521 kb on - strand at 525.550 kb on + strand, within leuL at 525.553 kb on + strand, within leuL at 525.554 kb on - strand, within leuL at 525.559 kb on + strand, within leuL at 525.559 kb on + strand, within leuL at 525.560 kb on - strand, within leuL at 525.756 kb on + strand at 525.910 kb on - strand, within NOLOHH_03060 at 525.910 kb on - strand, within NOLOHH_03060 at 525.910 kb on - strand, within NOLOHH_03060 at 525.910 kb on - strand, within NOLOHH_03060 at 525.910 kb on - strand, within NOLOHH_03060 at 525.919 kb on - strand, within NOLOHH_03060 at 525.941 kb on - strand, within NOLOHH_03060 at 525.998 kb on - strand, within NOLOHH_03060 at 526.066 kb on + strand, within NOLOHH_03060 at 526.066 kb on + strand, within NOLOHH_03060 at 526.070 kb on + strand, within NOLOHH_03060 at 526.070 kb on + strand, within NOLOHH_03060 at 526.070 kb on + strand, within NOLOHH_03060 at 526.070 kb on + strand, within NOLOHH_03060 at 526.070 kb on + strand, within NOLOHH_03060 at 526.071 kb on - strand, within NOLOHH_03060 at 526.071 kb on - strand, within NOLOHH_03060 at 526.071 kb on - strand, within NOLOHH_03060 at 526.071 kb on - strand, within NOLOHH_03060 at 526.071 kb on - strand, within NOLOHH_03060 at 526.071 kb on - strand, within NOLOHH_03060 at 526.071 kb on - strand, within NOLOHH_03060 at 526.071 kb on - strand, within NOLOHH_03060 at 526.177 kb on + strand, within NOLOHH_03060 at 526.272 kb on + strand, within NOLOHH_03060 at 526.273 kb on - strand, within NOLOHH_03060 at 526.276 kb on + strand, within NOLOHH_03060 at 526.276 kb on + strand, within NOLOHH_03060 at 526.277 kb on - strand, within NOLOHH_03060 at 526.277 kb on - strand, within NOLOHH_03060 at 526.294 kb on + strand, within NOLOHH_03060 at 526.295 kb on - strand, within NOLOHH_03060 at 526.302 kb on + strand, within NOLOHH_03060 at 526.306 kb on + strand, within NOLOHH_03060 at 526.306 kb on + strand, within NOLOHH_03060 at 526.307 kb on - strand, within NOLOHH_03060 at 526.307 kb on - strand, within NOLOHH_03060 at 526.307 kb on - strand, within NOLOHH_03060 at 526.307 kb on - strand, within NOLOHH_03060 at 526.321 kb on - strand, within NOLOHH_03060 at 526.354 kb on - strand, within NOLOHH_03060 at 526.354 kb on - strand, within NOLOHH_03060 at 526.354 kb on - strand, within NOLOHH_03060 at 526.497 kb on + strand, within NOLOHH_03060 at 526.498 kb on - strand, within NOLOHH_03060 at 526.515 kb on + strand, within NOLOHH_03060 at 526.516 kb on - strand, within NOLOHH_03060 at 526.516 kb on - strand, within NOLOHH_03060 at 526.516 kb on - strand, within NOLOHH_03060 at 526.531 kb on + strand, within NOLOHH_03060 at 526.531 kb on + strand, within NOLOHH_03060 at 526.532 kb on - strand, within NOLOHH_03060 at 526.534 kb on + strand, within NOLOHH_03060 at 526.535 kb on - strand, within NOLOHH_03060 at 526.631 kb on + strand, within NOLOHH_03060 at 526.737 kb on - strand, within NOLOHH_03060 at 526.738 kb on + strand, within NOLOHH_03060 at 526.739 kb on - strand, within NOLOHH_03060 at 526.817 kb on + strand, within NOLOHH_03060 at 526.883 kb on + strand, within NOLOHH_03060 at 526.907 kb on - strand, within NOLOHH_03060 at 526.998 kb on - strand, within NOLOHH_03060 at 527.024 kb on + strand, within NOLOHH_03060 at 527.024 kb on + strand, within NOLOHH_03060 at 527.025 kb on - strand, within NOLOHH_03060 at 527.025 kb on - strand, within NOLOHH_03060 at 527.025 kb on - strand, within NOLOHH_03060 at 527.026 kb on + strand, within NOLOHH_03060 at 527.026 kb on + strand, within NOLOHH_03060 at 527.026 kb on + strand, within NOLOHH_03060 at 527.045 kb on + strand, within NOLOHH_03060 at 527.108 kb on + strand, within NOLOHH_03060 at 527.184 kb on + strand at 527.184 kb on + strand at 527.184 kb on + strand at 527.185 kb on - strand at 527.194 kb on + strand at 527.194 kb on + strand at 527.290 kb on - strand at 527.313 kb on + strand at 527.399 kb on - strand, within leuA at 527.405 kb on + strand, within leuA at 527.405 kb on + strand, within leuA at 527.406 kb on - strand, within leuA at 527.522 kb on + strand, within leuA at 527.522 kb on + strand, within leuA at 527.582 kb on + strand, within leuA at 527.704 kb on + strand, within leuA at 527.704 kb on + strand, within leuA at 527.704 kb on + strand, within leuA at 527.704 kb on + strand, within leuA at 527.705 kb on - strand, within leuA at 527.743 kb on + strand, within leuA at 527.743 kb on + strand, within leuA at 527.743 kb on + strand, within leuA at 527.771 kb on - strand, within leuA at 527.771 kb on - strand, within leuA at 527.771 kb on - strand, within leuA at 527.771 kb on - strand, within leuA at 527.771 kb on - strand, within leuA at 527.771 kb on - strand, within leuA at 527.793 kb on + strand, within leuA at 527.794 kb on - strand, within leuA at 527.882 kb on + strand, within leuA at 527.883 kb on - strand, within leuA at 527.940 kb on + strand, within leuA at 527.940 kb on + strand, within leuA at 527.940 kb on + strand, within leuA at 527.940 kb on + strand, within leuA at 527.940 kb on + strand, within leuA at 527.940 kb on + strand, within leuA at 527.940 kb on + strand, within leuA at 527.941 kb on - strand, within leuA at 527.941 kb on - strand, within leuA at 527.941 kb on - strand, within leuA at 527.941 kb on - strand, within leuA at 527.941 kb on - strand, within leuA at 527.941 kb on - strand, within leuA at 527.944 kb on + strand, within leuA at 527.944 kb on + strand, within leuA at 527.944 kb on + strand, within leuA at 527.944 kb on + strand, within leuA at 527.944 kb on + strand, within leuA at 527.944 kb on + strand, within leuA at 527.944 kb on + strand, within leuA at 527.944 kb on + strand, within leuA at 527.944 kb on + strand, within leuA at 527.944 kb on + strand, within leuA at 527.944 kb on + strand, within leuA at 527.944 kb on + strand, within leuA at 527.944 kb on + strand, within leuA at 527.944 kb on + strand, within leuA at 527.945 kb on - strand, within leuA at 527.945 kb on - strand, within leuA at 527.945 kb on - strand, within leuA at 527.945 kb on - strand, within leuA at 527.945 kb on - strand, within leuA at 528.109 kb on + strand, within leuA at 528.109 kb on + strand, within leuA at 528.109 kb on + strand, within leuA at 528.109 kb on + strand, within leuA at 528.110 kb on - strand, within leuA at 528.260 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Rhamnose remove 524,721 + leuO NOLOHH_03050 0.85 -0.3 524,722 - leuO NOLOHH_03050 0.85 -0.7 524,785 + +0.1 524,785 + +1.5 524,785 + -0.6 524,786 - -0.7 524,786 - +1.3 524,806 + -0.3 524,807 - +0.5 524,807 - -1.6 524,807 - +0.8 524,884 + -0.7 524,884 + -1.1 524,885 - -0.1 524,937 + +0.6 524,937 + +0.7 524,938 - +0.8 525,052 - -0.9 525,097 - -0.2 525,117 + +0.2 525,196 - +0.6 525,237 + -1.0 525,329 + -3.4 525,359 - +0.9 525,402 + +0.2 525,488 + -0.9 525,489 - -2.3 525,496 + -0.7 525,520 + -4.6 525,521 - -3.2 525,550 + leuL NOLOHH_03055 0.32 -1.7 525,553 + leuL NOLOHH_03055 0.36 +0.5 525,554 - leuL NOLOHH_03055 0.37 -3.0 525,559 + leuL NOLOHH_03055 0.43 -0.2 525,559 + leuL NOLOHH_03055 0.43 +1.1 525,560 - leuL NOLOHH_03055 0.44 -2.5 525,756 + -3.7 525,910 - NOLOHH_03060 0.13 -1.5 525,910 - NOLOHH_03060 0.13 -4.1 525,910 - NOLOHH_03060 0.13 -4.5 525,910 - NOLOHH_03060 0.13 -4.8 525,910 - NOLOHH_03060 0.13 -4.0 525,919 - NOLOHH_03060 0.14 -4.5 525,941 - NOLOHH_03060 0.15 -3.3 525,998 - NOLOHH_03060 0.19 -5.6 526,066 + NOLOHH_03060 0.23 -3.5 526,066 + NOLOHH_03060 0.23 -3.6 526,070 + NOLOHH_03060 0.23 -4.5 526,070 + NOLOHH_03060 0.23 -4.4 526,070 + NOLOHH_03060 0.23 -5.2 526,070 + NOLOHH_03060 0.23 -2.8 526,070 + NOLOHH_03060 0.23 -4.0 526,071 - NOLOHH_03060 0.24 -4.8 526,071 - NOLOHH_03060 0.24 -5.2 526,071 - NOLOHH_03060 0.24 -4.4 526,071 - NOLOHH_03060 0.24 -3.8 526,071 - NOLOHH_03060 0.24 -3.5 526,071 - NOLOHH_03060 0.24 -3.7 526,071 - NOLOHH_03060 0.24 -3.3 526,071 - NOLOHH_03060 0.24 -4.3 526,177 + NOLOHH_03060 0.30 -4.7 526,272 + NOLOHH_03060 0.36 -4.0 526,273 - NOLOHH_03060 0.36 -2.6 526,276 + NOLOHH_03060 0.37 -2.4 526,276 + NOLOHH_03060 0.37 -3.6 526,277 - NOLOHH_03060 0.37 -1.8 526,277 - NOLOHH_03060 0.37 -3.8 526,294 + NOLOHH_03060 0.38 -5.5 526,295 - NOLOHH_03060 0.38 -4.5 526,302 + NOLOHH_03060 0.38 -5.1 526,306 + NOLOHH_03060 0.38 -5.8 526,306 + NOLOHH_03060 0.38 -1.5 526,307 - NOLOHH_03060 0.39 -3.1 526,307 - NOLOHH_03060 0.39 -3.6 526,307 - NOLOHH_03060 0.39 -4.7 526,307 - NOLOHH_03060 0.39 -5.0 526,321 - NOLOHH_03060 0.39 -3.7 526,354 - NOLOHH_03060 0.42 -3.9 526,354 - NOLOHH_03060 0.42 -3.6 526,354 - NOLOHH_03060 0.42 -3.2 526,497 + NOLOHH_03060 0.51 -3.7 526,498 - NOLOHH_03060 0.51 -5.1 526,515 + NOLOHH_03060 0.52 -4.8 526,516 - NOLOHH_03060 0.52 -3.3 526,516 - NOLOHH_03060 0.52 -3.3 526,516 - NOLOHH_03060 0.52 -4.6 526,531 + NOLOHH_03060 0.53 -4.0 526,531 + NOLOHH_03060 0.53 -5.2 526,532 - NOLOHH_03060 0.53 -4.5 526,534 + NOLOHH_03060 0.53 -5.5 526,535 - NOLOHH_03060 0.53 -0.9 526,631 + NOLOHH_03060 0.59 -3.6 526,737 - NOLOHH_03060 0.66 -4.0 526,738 + NOLOHH_03060 0.66 -5.9 526,739 - NOLOHH_03060 0.66 -3.4 526,817 + NOLOHH_03060 0.71 -4.7 526,883 + NOLOHH_03060 0.75 -0.0 526,907 - NOLOHH_03060 0.77 -3.2 526,998 - NOLOHH_03060 0.83 -2.7 527,024 + NOLOHH_03060 0.84 +0.0 527,024 + NOLOHH_03060 0.84 -0.6 527,025 - NOLOHH_03060 0.84 -5.3 527,025 - NOLOHH_03060 0.84 -4.5 527,025 - NOLOHH_03060 0.84 -5.1 527,026 + NOLOHH_03060 0.84 -3.8 527,026 + NOLOHH_03060 0.84 -3.9 527,026 + NOLOHH_03060 0.84 -3.9 527,045 + NOLOHH_03060 0.85 -2.8 527,108 + NOLOHH_03060 0.90 -2.4 527,184 + +0.8 527,184 + -0.3 527,184 + -3.8 527,185 - -3.7 527,194 + +0.7 527,194 + -2.3 527,290 - -2.7 527,313 + -4.5 527,399 - leuA NOLOHH_03065 0.12 -2.3 527,405 + leuA NOLOHH_03065 0.12 -3.2 527,405 + leuA NOLOHH_03065 0.12 -5.8 527,406 - leuA NOLOHH_03065 0.12 -4.4 527,522 + leuA NOLOHH_03065 0.23 -3.0 527,522 + leuA NOLOHH_03065 0.23 -3.4 527,582 + leuA NOLOHH_03065 0.28 -4.4 527,704 + leuA NOLOHH_03065 0.40 -2.6 527,704 + leuA NOLOHH_03065 0.40 -4.5 527,704 + leuA NOLOHH_03065 0.40 -4.8 527,704 + leuA NOLOHH_03065 0.40 -4.9 527,705 - leuA NOLOHH_03065 0.40 -0.6 527,743 + leuA NOLOHH_03065 0.43 -3.6 527,743 + leuA NOLOHH_03065 0.43 -3.5 527,743 + leuA NOLOHH_03065 0.43 -5.4 527,771 - leuA NOLOHH_03065 0.46 -4.6 527,771 - leuA NOLOHH_03065 0.46 -4.8 527,771 - leuA NOLOHH_03065 0.46 -5.2 527,771 - leuA NOLOHH_03065 0.46 -4.2 527,771 - leuA NOLOHH_03065 0.46 -3.7 527,771 - leuA NOLOHH_03065 0.46 -3.1 527,793 + leuA NOLOHH_03065 0.48 -2.1 527,794 - leuA NOLOHH_03065 0.48 -3.8 527,882 + leuA NOLOHH_03065 0.56 -2.0 527,883 - leuA NOLOHH_03065 0.56 -2.0 527,940 + leuA NOLOHH_03065 0.61 -3.5 527,940 + leuA NOLOHH_03065 0.61 -5.8 527,940 + leuA NOLOHH_03065 0.61 -4.9 527,940 + leuA NOLOHH_03065 0.61 -2.4 527,940 + leuA NOLOHH_03065 0.61 -4.3 527,940 + leuA NOLOHH_03065 0.61 -4.1 527,940 + leuA NOLOHH_03065 0.61 -2.0 527,941 - leuA NOLOHH_03065 0.61 -5.7 527,941 - leuA NOLOHH_03065 0.61 -3.7 527,941 - leuA NOLOHH_03065 0.61 -2.2 527,941 - leuA NOLOHH_03065 0.61 -4.7 527,941 - leuA NOLOHH_03065 0.61 -4.8 527,941 - leuA NOLOHH_03065 0.61 -1.4 527,944 + leuA NOLOHH_03065 0.62 -4.3 527,944 + leuA NOLOHH_03065 0.62 -4.5 527,944 + leuA NOLOHH_03065 0.62 -5.6 527,944 + leuA NOLOHH_03065 0.62 -5.6 527,944 + leuA NOLOHH_03065 0.62 -5.4 527,944 + leuA NOLOHH_03065 0.62 -5.4 527,944 + leuA NOLOHH_03065 0.62 -4.1 527,944 + leuA NOLOHH_03065 0.62 -4.6 527,944 + leuA NOLOHH_03065 0.62 -0.5 527,944 + leuA NOLOHH_03065 0.62 -5.1 527,944 + leuA NOLOHH_03065 0.62 -3.1 527,944 + leuA NOLOHH_03065 0.62 -5.2 527,944 + leuA NOLOHH_03065 0.62 -4.2 527,944 + leuA NOLOHH_03065 0.62 -4.7 527,945 - leuA NOLOHH_03065 0.62 -3.0 527,945 - leuA NOLOHH_03065 0.62 -4.3 527,945 - leuA NOLOHH_03065 0.62 -3.9 527,945 - leuA NOLOHH_03065 0.62 -4.1 527,945 - leuA NOLOHH_03065 0.62 -3.5 528,109 + leuA NOLOHH_03065 0.77 -4.4 528,109 + leuA NOLOHH_03065 0.77 -2.5 528,109 + leuA NOLOHH_03065 0.77 -3.1 528,109 + leuA NOLOHH_03065 0.77 -3.7 528,110 - leuA NOLOHH_03065 0.77 -5.1 528,260 + -3.9
Or see this region's nucleotide sequence