Strain Fitness in Escherichia coli ECOR27 around NOLOHH_00620

Experiment: L-Rhamnose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntNOLOHH_00610 and quuD are separated by 189 nucleotidesquuD and NOLOHH_00620 are separated by 62 nucleotidesNOLOHH_00620 and ylcG overlap by 4 nucleotidesylcG and rusA overlap by 1 nucleotidesrusA and ybcO overlap by 4 nucleotidesybcO and NOLOHH_00640 overlap by 8 nucleotidesNOLOHH_00640 and ybcN overlap by 1 nucleotides NOLOHH_00610: NOLOHH_00610 - Outer membrane porin protein LC, at 22,295 to 23,392 _00610 NOLOHH_00615: quuD - antitermination protein QuuD, at 23,582 to 23,965 quuD NOLOHH_00620: NOLOHH_00620 - Orf33, orf151, orf56, orf96, rus, orf45, orf8, orf127, and nmpC genes, at 24,028 to 24,054 _00620 NOLOHH_00625: ylcG - Uncharacterized protein YlcG, at 24,051 to 24,188 ylcG NOLOHH_00630: rusA - crossover junction endodeoxyribonuclease RusA, at 24,188 to 24,550 rusA NOLOHH_00635: ybcO - Putative nuclease YbcO, at 24,547 to 24,837 ybcO NOLOHH_00640: NOLOHH_00640 - NINE Protein, at 24,830 to 25,000 _00640 NOLOHH_00645: ybcN - DNA base-flipping protein YbcN, at 25,000 to 25,455 ybcN Position (kb) 24 25Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3at 23.062 kb on + strand, within NOLOHH_00610at 23.084 kb on - strand, within NOLOHH_00610at 23.084 kb on - strand, within NOLOHH_00610at 23.147 kb on + strand, within NOLOHH_00610at 23.232 kb on + strand, within NOLOHH_00610at 23.232 kb on + strand, within NOLOHH_00610at 23.240 kb on + strand, within NOLOHH_00610at 23.261 kb on + strand, within NOLOHH_00610at 23.261 kb on + strand, within NOLOHH_00610at 23.346 kb on + strandat 23.347 kb on - strandat 23.370 kb on + strandat 23.381 kb on + strandat 23.410 kb on + strandat 23.442 kb on + strandat 23.442 kb on + strandat 23.443 kb on - strandat 23.443 kb on - strandat 23.443 kb on - strandat 23.497 kb on + strandat 23.588 kb on - strandat 23.864 kb on + strand, within quuDat 23.972 kb on - strandat 24.029 kb on + strandat 24.041 kb on - strand, within NOLOHH_00620at 24.054 kb on + strandat 24.131 kb on - strand, within ylcGat 24.131 kb on - strand, within ylcGat 24.132 kb on + strand, within ylcGat 24.132 kb on + strand, within ylcGat 24.132 kb on + strand, within ylcGat 24.132 kb on + strand, within ylcGat 24.133 kb on - strand, within ylcGat 24.133 kb on - strand, within ylcGat 24.133 kb on - strand, within ylcGat 24.177 kb on - strandat 24.579 kb on + strand, within ybcOat 24.623 kb on + strand, within ybcOat 24.774 kb on + strand, within ybcOat 24.775 kb on - strand, within ybcOat 24.775 kb on - strand, within ybcOat 24.775 kb on - strand, within ybcOat 24.778 kb on + strand, within ybcOat 24.778 kb on + strand, within ybcOat 24.794 kb on - strand, within ybcOat 24.872 kb on - strand, within NOLOHH_00640at 24.889 kb on + strand, within NOLOHH_00640at 24.889 kb on + strand, within NOLOHH_00640at 24.890 kb on - strand, within NOLOHH_00640at 24.890 kb on - strand, within NOLOHH_00640at 24.890 kb on - strand, within NOLOHH_00640at 24.900 kb on - strand, within NOLOHH_00640at 24.909 kb on - strand, within NOLOHH_00640at 24.935 kb on - strand, within NOLOHH_00640at 24.935 kb on - strand, within NOLOHH_00640at 24.940 kb on + strand, within NOLOHH_00640at 24.960 kb on + strand, within NOLOHH_00640at 24.961 kb on - strand, within NOLOHH_00640at 24.994 kb on + strandat 24.995 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Rhamnose
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23,062 + NOLOHH_00610 0.70 +0.6
23,084 - NOLOHH_00610 0.72 +2.5
23,084 - NOLOHH_00610 0.72 +0.3
23,147 + NOLOHH_00610 0.78 +1.6
23,232 + NOLOHH_00610 0.85 -0.8
23,232 + NOLOHH_00610 0.85 -0.1
23,240 + NOLOHH_00610 0.86 +0.5
23,261 + NOLOHH_00610 0.88 +0.6
23,261 + NOLOHH_00610 0.88 +1.4
23,346 + -0.8
23,347 - +0.2
23,370 + -0.7
23,381 + +0.2
23,410 + -5.1
23,442 + -0.9
23,442 + +0.5
23,443 - +1.3
23,443 - +1.2
23,443 - +0.3
23,497 + -0.8
23,588 - -3.9
23,864 + quuD NOLOHH_00615 0.73 +0.2
23,972 - +1.0
24,029 + -2.0
24,041 - NOLOHH_00620 0.48 -2.6
24,054 + -1.3
24,131 - ylcG NOLOHH_00625 0.58 +0.6
24,131 - ylcG NOLOHH_00625 0.58 +0.5
24,132 + ylcG NOLOHH_00625 0.59 -1.0
24,132 + ylcG NOLOHH_00625 0.59 -4.4
24,132 + ylcG NOLOHH_00625 0.59 +0.6
24,132 + ylcG NOLOHH_00625 0.59 -2.4
24,133 - ylcG NOLOHH_00625 0.59 -0.5
24,133 - ylcG NOLOHH_00625 0.59 +0.4
24,133 - ylcG NOLOHH_00625 0.59 +0.1
24,177 - +1.0
24,579 + ybcO NOLOHH_00635 0.11 -0.4
24,623 + ybcO NOLOHH_00635 0.26 +0.4
24,774 + ybcO NOLOHH_00635 0.78 +0.1
24,775 - ybcO NOLOHH_00635 0.78 +2.4
24,775 - ybcO NOLOHH_00635 0.78 +0.2
24,775 - ybcO NOLOHH_00635 0.78 +0.0
24,778 + ybcO NOLOHH_00635 0.79 -0.5
24,778 + ybcO NOLOHH_00635 0.79 +0.8
24,794 - ybcO NOLOHH_00635 0.85 +2.8
24,872 - NOLOHH_00640 0.25 +0.8
24,889 + NOLOHH_00640 0.35 -0.4
24,889 + NOLOHH_00640 0.35 -0.3
24,890 - NOLOHH_00640 0.35 +0.0
24,890 - NOLOHH_00640 0.35 -0.3
24,890 - NOLOHH_00640 0.35 -0.5
24,900 - NOLOHH_00640 0.41 -0.1
24,909 - NOLOHH_00640 0.46 -0.2
24,935 - NOLOHH_00640 0.61 -3.2
24,935 - NOLOHH_00640 0.61 +0.3
24,940 + NOLOHH_00640 0.64 -1.2
24,960 + NOLOHH_00640 0.76 -2.5
24,961 - NOLOHH_00640 0.77 -0.2
24,994 + +0.6
24,995 - -0.2

Or see this region's nucleotide sequence