Strain Fitness in Escherichia coli ECRC100 around OKFHMN_28460

Experiment: Sodium-DL-Lactate

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntprfA and prmC overlap by 1 nucleotidesprmC and sirB2 overlap by 4 nucleotidessirB2 and sirB1 are separated by 3 nucleotidessirB1 and kdsA are separated by 35 nucleotides OKFHMN_28450: prfA - peptide chain release factor 1, at 5,459,511 to 5,460,593 prfA OKFHMN_28455: prmC - peptide chain release factor N(5)-glutamine methyltransferase, at 5,460,593 to 5,461,426 prmC OKFHMN_28460: sirB2 - invasion regulator SirB2, at 5,461,423 to 5,461,815 sirB2 OKFHMN_28465: sirB1 - invasion regulator SirB1, at 5,461,819 to 5,462,628 sirB1 OKFHMN_28470: kdsA - 3-deoxy-8-phosphooctulonate synthase, at 5,462,664 to 5,463,518 kdsA Position (kb) 5461 5462Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 5461.461 kb on + strandat 5461.606 kb on - strand, within sirB2at 5461.768 kb on + strand, within sirB2at 5461.799 kb on + strandat 5461.944 kb on + strand, within sirB1at 5462.268 kb on + strand, within sirB1at 5462.279 kb on + strand, within sirB1at 5462.591 kb on + strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
remove
5,461,461 + -1.9
5,461,606 - sirB2 OKFHMN_28460 0.47 +0.2
5,461,768 + sirB2 OKFHMN_28460 0.88 +0.5
5,461,799 + +0.1
5,461,944 + sirB1 OKFHMN_28465 0.15 +0.2
5,462,268 + sirB1 OKFHMN_28465 0.55 +2.2
5,462,279 + sirB1 OKFHMN_28465 0.57 -2.8
5,462,591 + -1.1

Or see this region's nucleotide sequence