Strain Fitness in Escherichia coli ECRC100 around OKFHMN_28390

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttreA and dhaM are separated by 546 nucleotidesdhaM and dhaL are separated by 10 nucleotidesdhaL and dhaK are separated by 10 nucleotides OKFHMN_28385: treA - Periplasmic trehalase, at 5,443,252 to 5,444,709 treA OKFHMN_28390: dhaM - dihydroxyacetone kinase phosphoryl donor subunit DhaM, at 5,445,256 to 5,446,674 dhaM OKFHMN_28395: dhaL - dihydroxyacetone kinase subunit DhaL, at 5,446,685 to 5,447,317 dhaL OKFHMN_28400: dhaK - dihydroxyacetone kinase subunit DhaK, at 5,447,328 to 5,448,398 dhaK Position (kb) 5445 5446 5447Strain fitness (log2 ratio) -2 -1 0 1 2at 5444.478 kb on - strand, within treAat 5444.480 kb on + strand, within treAat 5444.483 kb on - strand, within treAat 5445.074 kb on + strandat 5446.647 kb on + strandat 5446.896 kb on - strand, within dhaLat 5447.144 kb on - strand, within dhaLat 5447.330 kb on - strandat 5447.427 kb on - strandat 5447.444 kb on - strand, within dhaK

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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5,444,478 - treA OKFHMN_28385 0.84 +1.9
5,444,480 + treA OKFHMN_28385 0.84 +0.3
5,444,483 - treA OKFHMN_28385 0.84 +0.4
5,445,074 + -1.8
5,446,647 + -0.0
5,446,896 - dhaL OKFHMN_28395 0.33 +0.3
5,447,144 - dhaL OKFHMN_28395 0.73 +1.2
5,447,330 - -0.1
5,447,427 - +0.7
5,447,444 - dhaK OKFHMN_28400 0.11 -0.5

Or see this region's nucleotide sequence