Strain Fitness in Escherichia coli ECRC100 around OKFHMN_28290

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycgB and dadA are separated by 362 nucleotidesdadA and dadX are separated by 9 nucleotides OKFHMN_28285: ycgB - SpoVR family protein, at 5,424,497 to 5,426,029 ycgB OKFHMN_28290: dadA - D-amino acid dehydrogenase, at 5,426,392 to 5,427,690 dadA OKFHMN_28295: dadX - catabolic alanine racemase DadX, at 5,427,700 to 5,428,770 dadX Position (kb) 5426 5427 5428Strain fitness (log2 ratio) -2 -1 0 1at 5425.691 kb on - strand, within ycgBat 5425.776 kb on + strand, within ycgBat 5425.903 kb on + strandat 5426.175 kb on - strandat 5426.247 kb on - strandat 5426.247 kb on - strandat 5426.344 kb on + strandat 5426.369 kb on + strandat 5426.369 kb on + strandat 5426.779 kb on + strand, within dadAat 5426.779 kb on + strand, within dadAat 5427.040 kb on - strand, within dadAat 5427.872 kb on + strand, within dadXat 5427.999 kb on - strand, within dadXat 5428.404 kb on - strand, within dadXat 5428.471 kb on - strand, within dadX

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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5,425,691 - ycgB OKFHMN_28285 0.78 -0.0
5,425,776 + ycgB OKFHMN_28285 0.83 -0.9
5,425,903 + +0.3
5,426,175 - +0.2
5,426,247 - +0.9
5,426,247 - -0.0
5,426,344 + +0.3
5,426,369 + -2.2
5,426,369 + -0.3
5,426,779 + dadA OKFHMN_28290 0.30 +0.4
5,426,779 + dadA OKFHMN_28290 0.30 +1.0
5,427,040 - dadA OKFHMN_28290 0.50 +1.5
5,427,872 + dadX OKFHMN_28295 0.16 -0.2
5,427,999 - dadX OKFHMN_28295 0.28 +0.8
5,428,404 - dadX OKFHMN_28295 0.66 +1.0
5,428,471 - dadX OKFHMN_28295 0.72 -0.1

Or see this region's nucleotide sequence