Strain Fitness in Escherichia coli ECRC100 around OKFHMN_26795

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOKFHMN_26785 and tnp-IS629 overlap by 1 nucleotidestnp-IS629 and ydjE are separated by 152 nucleotidesydjE and pncA are separated by 92 nucleotidespncA and ansA are separated by 10 nucleotides OKFHMN_26785: OKFHMN_26785 - IS3 family transposase, at 5,154,969 to 5,155,856 _26785 OKFHMN_26790: tnp-IS629 - IS3 family IS629 transposase, at 5,155,856 to 5,156,182 tnp-IS629 OKFHMN_26795: ydjE - Inner membrane metabolite transport protein YdjE, at 5,156,335 to 5,157,495 ydjE OKFHMN_26800: pncA - bifunctional nicotinamidase/pyrazinamidase, at 5,157,588 to 5,158,229 pncA OKFHMN_26805: ansA - asparaginase, at 5,158,240 to 5,159,256 ansA Position (kb) 5156 5157 5158Strain fitness (log2 ratio) -2 -1 0 1at 5157.394 kb on - strandat 5157.563 kb on + strandat 5157.564 kb on - strandat 5157.621 kb on - strandat 5157.725 kb on - strand, within pncAat 5157.767 kb on - strand, within pncAat 5157.767 kb on - strand, within pncAat 5157.871 kb on + strand, within pncAat 5157.871 kb on + strand, within pncAat 5157.872 kb on - strand, within pncAat 5158.318 kb on - strandat 5158.318 kb on - strandat 5158.352 kb on - strand, within ansAat 5158.438 kb on - strand, within ansAat 5158.438 kb on - strand, within ansA

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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5,157,394 - +0.3
5,157,563 + +0.4
5,157,564 - +0.3
5,157,621 - -1.2
5,157,725 - pncA OKFHMN_26800 0.21 -2.7
5,157,767 - pncA OKFHMN_26800 0.28 +0.5
5,157,767 - pncA OKFHMN_26800 0.28 +0.3
5,157,871 + pncA OKFHMN_26800 0.44 -0.9
5,157,871 + pncA OKFHMN_26800 0.44 +1.4
5,157,872 - pncA OKFHMN_26800 0.44 -1.8
5,158,318 - +1.2
5,158,318 - -0.6
5,158,352 - ansA OKFHMN_26805 0.11 +0.2
5,158,438 - ansA OKFHMN_26805 0.19 +0.7
5,158,438 - ansA OKFHMN_26805 0.19 +0.4

Or see this region's nucleotide sequence