Strain Fitness in Escherichia coli ECRC100 around OKFHMN_26685

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnimT and nimR are separated by 99 nucleotidesnimR and yeaL overlap by 44 nucleotidesyeaL and yeaK are separated by 274 nucleotidesyeaK and dgcJ are separated by 42 nucleotides OKFHMN_26675: nimT - 2-nitroimidazole transporter, at 5,131,960 to 5,133,141 nimT OKFHMN_26680: nimR - DNA-binding transcriptional regulator NimR, at 5,133,241 to 5,134,059 nimR OKFHMN_26685: yeaL - UPF0756 membrane protein CKO_01811, at 5,134,016 to 5,134,462 yeaL OKFHMN_26690: yeaK - mischarged aminoacyl-tRNA deacylase, at 5,134,737 to 5,135,240 yeaK OKFHMN_26695: dgcJ - diguanylate cyclase DgcJ, at 5,135,283 to 5,136,773 dgcJ Position (kb) 5134 5135Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 5133.377 kb on + strand, within nimRat 5133.465 kb on + strand, within nimRat 5133.866 kb on + strand, within nimRat 5133.963 kb on + strand, within nimRat 5133.963 kb on + strand, within nimRat 5133.969 kb on + strand, within nimRat 5134.002 kb on + strandat 5134.003 kb on - strandat 5134.735 kb on - strandat 5134.984 kb on - strand, within yeaKat 5135.073 kb on - strand, within yeaKat 5135.077 kb on - strand, within yeaKat 5135.077 kb on - strand, within yeaKat 5135.318 kb on - strandat 5135.352 kb on + strandat 5135.353 kb on - strandat 5135.382 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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5,133,377 + nimR OKFHMN_26680 0.17 +1.2
5,133,465 + nimR OKFHMN_26680 0.27 -1.0
5,133,866 + nimR OKFHMN_26680 0.76 +0.1
5,133,963 + nimR OKFHMN_26680 0.88 -0.1
5,133,963 + nimR OKFHMN_26680 0.88 -0.2
5,133,969 + nimR OKFHMN_26680 0.89 +1.8
5,134,002 + +1.2
5,134,003 - +0.8
5,134,735 - +0.6
5,134,984 - yeaK OKFHMN_26690 0.49 -0.2
5,135,073 - yeaK OKFHMN_26690 0.67 -0.1
5,135,077 - yeaK OKFHMN_26690 0.67 -3.6
5,135,077 - yeaK OKFHMN_26690 0.67 +0.0
5,135,318 - -1.3
5,135,352 + -1.4
5,135,353 - +0.2
5,135,382 - +0.9

Or see this region's nucleotide sequence