Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt edd and kdgA are separated by 36 nucleotides kdgA and purT are separated by 55 nucleotides purT and yebG are separated by 133 nucleotides yebG and yebF are separated by 66 nucleotides yebF and OKFHMN_26365 overlap by 87 nucleotides
OKFHMN_26340: edd - phosphogluconate dehydratase, at 5,073,984 to 5,075,795
edd
OKFHMN_26345: kdgA - bifunctional 4-hydroxy-2-oxoglutarate aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase, at 5,075,832 to 5,076,473
kdgA
OKFHMN_26350: purT - formate-dependent phosphoribosylglycinamide formyltransferase, at 5,076,529 to 5,077,707
purT
OKFHMN_26355: yebG - DNA damage-inducible protein YebG, at 5,077,841 to 5,078,131
yebG
OKFHMN_26360: yebF - Protein YebF, at 5,078,198 to 5,078,554
yebF
OKFHMN_26365: OKFHMN_26365 - hypothetical protein, at 5,078,468 to 5,078,674
_26365
Position (kb)
5076
5077
5078 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5075.558 kb on - strand, within edd at 5075.794 kb on - strand at 5076.155 kb on + strand, within kdgA at 5076.156 kb on - strand, within kdgA at 5076.211 kb on - strand, within kdgA at 5076.320 kb on + strand, within kdgA at 5076.336 kb on + strand, within kdgA at 5076.337 kb on - strand, within kdgA at 5076.454 kb on + strand at 5077.132 kb on + strand, within purT at 5077.476 kb on - strand, within purT at 5077.528 kb on - strand, within purT at 5077.531 kb on - strand, within purT at 5077.655 kb on - strand at 5077.658 kb on - strand at 5077.725 kb on + strand at 5077.859 kb on + strand at 5077.859 kb on + strand at 5077.860 kb on - strand at 5077.863 kb on + strand at 5077.863 kb on + strand at 5077.939 kb on + strand, within yebG at 5078.016 kb on + strand, within yebG at 5078.443 kb on + strand, within yebF at 5078.475 kb on + strand at 5078.501 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 5,075,558 - edd OKFHMN_26340 0.87 +0.7 5,075,794 - -0.2 5,076,155 + kdgA OKFHMN_26345 0.50 +2.5 5,076,156 - kdgA OKFHMN_26345 0.50 -0.0 5,076,211 - kdgA OKFHMN_26345 0.59 +1.9 5,076,320 + kdgA OKFHMN_26345 0.76 +0.5 5,076,336 + kdgA OKFHMN_26345 0.79 -1.8 5,076,337 - kdgA OKFHMN_26345 0.79 -0.1 5,076,454 + +1.3 5,077,132 + purT OKFHMN_26350 0.51 -0.1 5,077,476 - purT OKFHMN_26350 0.80 +0.5 5,077,528 - purT OKFHMN_26350 0.85 -1.0 5,077,531 - purT OKFHMN_26350 0.85 +0.5 5,077,655 - -0.8 5,077,658 - +0.4 5,077,725 + -0.1 5,077,859 + -0.2 5,077,859 + +0.5 5,077,860 - -0.3 5,077,863 + -0.8 5,077,863 + -1.9 5,077,939 + yebG OKFHMN_26355 0.34 -0.2 5,078,016 + yebG OKFHMN_26355 0.60 +2.2 5,078,443 + yebF OKFHMN_26360 0.69 +0.5 5,078,475 + +0.2 5,078,501 + -0.4
Or see this region's nucleotide sequence