Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aspS and nudB are separated by 60 nucleotides nudB and OKFHMN_26255 are separated by 28 nucleotides OKFHMN_26255 and OKFHMN_26260 overlap by 1 nucleotides OKFHMN_26260 and ruvC overlap by 1 nucleotides
OKFHMN_26245: aspS - aspartate--tRNA ligase, at 5,057,967 to 5,059,739
aspS
OKFHMN_26250: nudB - dihydroneopterin triphosphate diphosphatase, at 5,059,800 to 5,060,309
nudB
OKFHMN_26255: OKFHMN_26255 - putative transcriptional regulatory protein YebC, at 5,060,338 to 5,061,078
_26255
OKFHMN_26260: OKFHMN_26260 - Crossover junction endodeoxyribonuclease RuvC, at 5,061,078 to 5,061,113
_26260
OKFHMN_26265: ruvC - crossover junction endodeoxyribonuclease RuvC, at 5,061,113 to 5,061,634
ruvC
Position (kb) 5059
5060
5061 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 5059.740 kb on + strand at 5059.807 kb on + strand at 5059.807 kb on + strand at 5059.811 kb on + strand at 5059.811 kb on + strand at 5059.815 kb on + strand at 5059.815 kb on + strand at 5059.815 kb on + strand at 5059.865 kb on + strand, within nudB at 5059.872 kb on + strand, within nudB at 5059.894 kb on + strand, within nudB at 5059.905 kb on + strand, within nudB at 5059.916 kb on + strand, within nudB at 5060.026 kb on + strand, within nudB at 5060.071 kb on + strand, within nudB at 5060.116 kb on + strand, within nudB at 5060.293 kb on + strand at 5060.415 kb on + strand, within OKFHMN_26255 at 5060.830 kb on + strand, within OKFHMN_26255 at 5060.854 kb on + strand, within OKFHMN_26255 at 5060.854 kb on + strand, within OKFHMN_26255
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 5,059,740 + +1.0 5,059,807 + +0.2 5,059,807 + -4.0 5,059,811 + +0.0 5,059,811 + -0.9 5,059,815 + -0.2 5,059,815 + -0.1 5,059,815 + -0.5 5,059,865 + nudB OKFHMN_26250 0.13 -3.4 5,059,872 + nudB OKFHMN_26250 0.14 -1.5 5,059,894 + nudB OKFHMN_26250 0.18 -1.9 5,059,905 + nudB OKFHMN_26250 0.21 -0.7 5,059,916 + nudB OKFHMN_26250 0.23 -3.4 5,060,026 + nudB OKFHMN_26250 0.44 -2.1 5,060,071 + nudB OKFHMN_26250 0.53 -1.1 5,060,116 + nudB OKFHMN_26250 0.62 -1.4 5,060,293 + -1.4 5,060,415 + OKFHMN_26255 0.10 -0.8 5,060,830 + OKFHMN_26255 0.66 -0.5 5,060,854 + OKFHMN_26255 0.70 -0.8 5,060,854 + OKFHMN_26255 0.70 -0.3
Or see this region's nucleotide sequence