Strain Fitness in Escherichia coli ECRC100 around OKFHMN_26225

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcmoB and cmoA overlap by 4 nucleotidescmoA and yecN are separated by 40 nucleotidesyecN and yecE are separated by 52 nucleotides OKFHMN_26220: cmoB - tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB, at 5,054,076 to 5,055,047 cmoB OKFHMN_26225: cmoA - carboxy-S-adenosyl-L-methionine synthase CmoA, at 5,055,044 to 5,055,787 cmoA OKFHMN_26230: yecN - Inner membrane protein YecN, at 5,055,828 to 5,056,223 yecN OKFHMN_26235: yecE - UPF0759 protein YecE, at 5,056,276 to 5,057,094 yecE Position (kb) 5055 5056Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 5054.245 kb on - strand, within cmoBat 5054.759 kb on - strand, within cmoBat 5054.930 kb on + strand, within cmoBat 5055.437 kb on - strand, within cmoAat 5055.456 kb on - strand, within cmoAat 5055.545 kb on - strand, within cmoAat 5055.545 kb on - strand, within cmoAat 5055.647 kb on - strand, within cmoAat 5055.647 kb on - strand, within cmoAat 5055.861 kb on - strandat 5055.863 kb on - strandat 5055.999 kb on - strand, within yecNat 5056.065 kb on - strand, within yecNat 5056.224 kb on - strandat 5056.259 kb on + strandat 5056.265 kb on - strandat 5056.356 kb on + strandat 5056.356 kb on + strandat 5056.360 kb on + strand, within yecEat 5056.383 kb on + strand, within yecEat 5056.407 kb on - strand, within yecEat 5056.494 kb on + strand, within yecEat 5056.535 kb on - strand, within yecE

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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5,054,245 - cmoB OKFHMN_26220 0.17 +0.4
5,054,759 - cmoB OKFHMN_26220 0.70 +1.3
5,054,930 + cmoB OKFHMN_26220 0.88 -0.4
5,055,437 - cmoA OKFHMN_26225 0.53 -1.2
5,055,456 - cmoA OKFHMN_26225 0.55 +1.1
5,055,545 - cmoA OKFHMN_26225 0.67 -0.5
5,055,545 - cmoA OKFHMN_26225 0.67 +0.4
5,055,647 - cmoA OKFHMN_26225 0.81 +0.3
5,055,647 - cmoA OKFHMN_26225 0.81 -0.1
5,055,861 - -2.1
5,055,863 - +0.2
5,055,999 - yecN OKFHMN_26230 0.43 +1.8
5,056,065 - yecN OKFHMN_26230 0.60 +2.1
5,056,224 - -0.7
5,056,259 + -0.6
5,056,265 - -0.8
5,056,356 + -2.3
5,056,356 + +0.8
5,056,360 + yecE OKFHMN_26235 0.10 -0.7
5,056,383 + yecE OKFHMN_26235 0.13 +1.4
5,056,407 - yecE OKFHMN_26235 0.16 -0.6
5,056,494 + yecE OKFHMN_26235 0.27 +0.3
5,056,535 - yecE OKFHMN_26235 0.32 -3.3

Or see this region's nucleotide sequence