Strain Fitness in Escherichia coli ECRC100 around OKFHMN_26020

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntxerD and OKFHMN_26015 are separated by 245 nucleotidesOKFHMN_26015 and tnp-IS3 overlap by 35 nucleotidestnp-IS3 and tnp-IS3 overlap by 1 nucleotidestnp-IS3 and yecA are separated by 162 nucleotides OKFHMN_26010: xerD - integrase, at 5,014,976 to 5,015,977 xerD OKFHMN_26015: OKFHMN_26015 - AMP nucleosidase, at 5,016,223 to 5,016,306 _26015 OKFHMN_26020: tnp-IS3 - IS3 family transposase, at 5,016,272 to 5,017,159 tnp-IS3 OKFHMN_26025: tnp-IS3 - IS3 family transposase, at 5,017,159 to 5,017,485 tnp-IS3 OKFHMN_26030: yecA - UPF0149 family protein YecA, at 5,017,648 to 5,018,313 yecA Position (kb) 5016 5017 5018Strain fitness (log2 ratio) -2 -1 0 1at 5015.290 kb on + strand, within xerDat 5015.347 kb on + strand, within xerDat 5015.741 kb on + strand, within xerDat 5016.001 kb on + strandat 5016.262 kb on - strand, within OKFHMN_26015at 5017.606 kb on + strandat 5017.708 kb on + strandat 5017.872 kb on + strand, within yecAat 5017.873 kb on - strand, within yecAat 5017.893 kb on + strand, within yecAat 5018.150 kb on + strand, within yecAat 5018.150 kb on + strand, within yecA

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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5,015,290 + xerD OKFHMN_26010 0.31 -0.4
5,015,347 + xerD OKFHMN_26010 0.37 +0.3
5,015,741 + xerD OKFHMN_26010 0.76 -2.7
5,016,001 + +0.6
5,016,262 - OKFHMN_26015 0.46 +0.3
5,017,606 + -0.0
5,017,708 + -1.2
5,017,872 + yecA OKFHMN_26030 0.34 +0.5
5,017,873 - yecA OKFHMN_26030 0.34 -0.0
5,017,893 + yecA OKFHMN_26030 0.37 +1.7
5,018,150 + yecA OKFHMN_26030 0.75 +0.9
5,018,150 + yecA OKFHMN_26030 0.75 -0.7

Or see this region's nucleotide sequence