Strain Fitness in Escherichia coli ECRC100 around OKFHMN_25915

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttnp-IS629 and tnp-IS3 overlap by 1 nucleotidestnp-IS3 and OKFHMN_25915 are separated by 19 nucleotidesOKFHMN_25915 and OKFHMN_25920 are separated by 4 nucleotides OKFHMN_25905: tnp-IS629 - IS3 family IS629 transposase, at 5,004,491 to 5,004,817 tnp-IS629 OKFHMN_25910: tnp-IS3 - IS3 family transposase, at 5,004,817 to 5,005,506 tnp-IS3 OKFHMN_25915: OKFHMN_25915 - peptide transporter, at 5,005,526 to 5,005,840 _25915 OKFHMN_25920: OKFHMN_25920 - recombinase, at 5,005,845 to 5,006,804 _25920 Position (kb) 5005 5006Strain fitness (log2 ratio) -2 -1 0 1 2at 5005.497 kb on - strandat 5005.527 kb on + strandat 5005.598 kb on - strand, within OKFHMN_25915at 5005.605 kb on + strand, within OKFHMN_25915at 5005.691 kb on + strand, within OKFHMN_25915at 5005.764 kb on + strand, within OKFHMN_25915at 5005.864 kb on + strandat 5006.588 kb on - strand, within OKFHMN_25920at 5006.666 kb on - strand, within OKFHMN_25920

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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5,005,497 - +0.0
5,005,527 + +2.2
5,005,598 - OKFHMN_25915 0.23 -1.9
5,005,605 + OKFHMN_25915 0.25 +1.1
5,005,691 + OKFHMN_25915 0.52 +0.6
5,005,764 + OKFHMN_25915 0.76 +0.6
5,005,864 + -0.6
5,006,588 - OKFHMN_25920 0.77 +0.3
5,006,666 - OKFHMN_25920 0.86 +0.3

Or see this region's nucleotide sequence