Strain Fitness in Escherichia coli ECRC100 around OKFHMN_25150

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntldtA and Asn_trna are separated by 55 nucleotidesAsn_trna and nac are separated by 325 nucleotidesnac and cbl are separated by 101 nucleotides OKFHMN_25140: ldtA - L,D-transpeptidase, at 4,878,332 to 4,879,267 ldtA OKFHMN_25145: Asn_trna - tRNA-Asn(gtt), at 4,879,323 to 4,879,398 _trna OKFHMN_25150: nac - nitrogen assimilation transcriptional regulator NAC, at 4,879,724 to 4,880,641 nac OKFHMN_25155: cbl - HTH-type transcriptional regulator Cbl, at 4,880,743 to 4,881,693 cbl Position (kb) 4879 4880 4881Strain fitness (log2 ratio) -2 -1 0 1 2at 4878.881 kb on + strand, within ldtAat 4878.896 kb on + strand, within ldtAat 4879.005 kb on + strand, within ldtAat 4879.010 kb on - strand, within ldtAat 4879.266 kb on - strandat 4879.282 kb on - strandat 4879.609 kb on + strandat 4879.631 kb on + strandat 4879.678 kb on + strandat 4879.682 kb on + strandat 4880.055 kb on + strand, within nacat 4880.055 kb on + strand, within nacat 4880.093 kb on + strand, within nacat 4880.166 kb on - strand, within nacat 4880.527 kb on - strand, within nacat 4880.661 kb on + strandat 4880.705 kb on + strandat 4880.759 kb on + strandat 4880.759 kb on + strandat 4881.012 kb on + strand, within cblat 4881.061 kb on - strand, within cblat 4881.286 kb on - strand, within cblat 4881.403 kb on - strand, within cblat 4881.404 kb on + strand, within cblat 4881.404 kb on + strand, within cblat 4881.405 kb on - strand, within cblat 4881.439 kb on + strand, within cblat 4881.544 kb on - strand, within cblat 4881.586 kb on - strand, within cblat 4881.589 kb on + strand, within cbl

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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4,878,881 + ldtA OKFHMN_25140 0.59 +0.4
4,878,896 + ldtA OKFHMN_25140 0.60 +0.9
4,879,005 + ldtA OKFHMN_25140 0.72 -1.1
4,879,010 - ldtA OKFHMN_25140 0.72 +2.1
4,879,266 - +0.0
4,879,282 - +0.8
4,879,609 + -2.3
4,879,631 + +0.5
4,879,678 + +0.0
4,879,682 + +1.0
4,880,055 + nac OKFHMN_25150 0.36 -0.6
4,880,055 + nac OKFHMN_25150 0.36 -2.1
4,880,093 + nac OKFHMN_25150 0.40 -0.7
4,880,166 - nac OKFHMN_25150 0.48 +0.3
4,880,527 - nac OKFHMN_25150 0.87 +1.0
4,880,661 + +0.5
4,880,705 + +0.4
4,880,759 + +0.3
4,880,759 + +0.1
4,881,012 + cbl OKFHMN_25155 0.28 +1.4
4,881,061 - cbl OKFHMN_25155 0.33 +0.3
4,881,286 - cbl OKFHMN_25155 0.57 -0.5
4,881,403 - cbl OKFHMN_25155 0.69 -0.2
4,881,404 + cbl OKFHMN_25155 0.70 +0.7
4,881,404 + cbl OKFHMN_25155 0.70 -0.2
4,881,405 - cbl OKFHMN_25155 0.70 +1.5
4,881,439 + cbl OKFHMN_25155 0.73 +0.1
4,881,544 - cbl OKFHMN_25155 0.84 -0.3
4,881,586 - cbl OKFHMN_25155 0.89 +1.5
4,881,589 + cbl OKFHMN_25155 0.89 +0.5

Or see this region's nucleotide sequence