Strain Fitness in Escherichia coli ECRC100 around OKFHMN_24820

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntstxA2c and stxB2c are separated by 11 nucleotidesstxB2c and iraM are separated by 296 nucleotidesiraM and OKFHMN_24825 are separated by 243 nucleotides OKFHMN_24805: stxA2c - Shiga toxin Stx2c subunit A, at 4,821,867 to 4,822,826 stxA2c OKFHMN_24810: stxB2c - Shiga toxin Stx2c subunit B, at 4,822,838 to 4,823,107 stxB2c OKFHMN_24820: iraM - Anti-adapter protein IraM, at 4,823,404 to 4,823,727 iraM OKFHMN_24825: OKFHMN_24825 - sialate O-acetylesterase, at 4,823,971 to 4,825,908 _24825 Position (kb) 4823 4824Strain fitness (log2 ratio) -2 -1 0 1at 4822.751 kb on + strandat 4822.770 kb on - strandat 4823.127 kb on + strandat 4823.250 kb on - strandat 4823.338 kb on + strandat 4823.338 kb on + strandat 4823.339 kb on - strandat 4823.355 kb on - strandat 4823.543 kb on + strand, within iraMat 4823.544 kb on - strand, within iraMat 4823.659 kb on - strand, within iraMat 4823.979 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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4,822,751 + +0.3
4,822,770 - -0.8
4,823,127 + +0.6
4,823,250 - -0.3
4,823,338 + -0.2
4,823,338 + -1.1
4,823,339 - +0.3
4,823,355 - -2.1
4,823,543 + iraM OKFHMN_24820 0.43 -0.8
4,823,544 - iraM OKFHMN_24820 0.43 -0.2
4,823,659 - iraM OKFHMN_24820 0.79 -1.1
4,823,979 + -0.5

Or see this region's nucleotide sequence