Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wcaK and wcaL overlap by 4 nucleotides wcaL and wcaM are separated by 10 nucleotides
OKFHMN_24385: wcaK - colanic acid biosynthesis pyruvyl transferase WcaK, at 4,762,010 to 4,763,290
wcaK
OKFHMN_24390: wcaL - colanic acid biosynthesis glycosyltransferase WcaL, at 4,763,287 to 4,764,507
wcaL
OKFHMN_24395: wcaM - colanic acid biosynthesis protein WcaM, at 4,764,518 to 4,765,912
wcaM
Position (kb)
4763
4764
4765 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4762.372 kb on + strand, within wcaK at 4762.466 kb on - strand, within wcaK at 4762.673 kb on + strand, within wcaK at 4762.673 kb on + strand, within wcaK at 4762.674 kb on - strand, within wcaK at 4762.856 kb on - strand, within wcaK at 4762.872 kb on - strand, within wcaK at 4762.874 kb on - strand, within wcaK at 4762.941 kb on + strand, within wcaK at 4762.941 kb on + strand, within wcaK at 4762.941 kb on + strand, within wcaK at 4762.942 kb on - strand, within wcaK at 4763.069 kb on + strand, within wcaK at 4763.139 kb on + strand, within wcaK at 4763.368 kb on - strand at 4763.440 kb on + strand, within wcaL at 4763.777 kb on - strand, within wcaL at 4763.962 kb on - strand, within wcaL at 4763.962 kb on - strand, within wcaL at 4764.109 kb on + strand, within wcaL at 4764.286 kb on - strand, within wcaL at 4764.504 kb on - strand at 4764.517 kb on + strand at 4764.939 kb on - strand, within wcaM at 4765.084 kb on - strand, within wcaM at 4765.168 kb on + strand, within wcaM at 4765.169 kb on - strand, within wcaM at 4765.229 kb on - strand, within wcaM at 4765.409 kb on + strand, within wcaM
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 4,762,372 + wcaK OKFHMN_24385 0.28 -0.4 4,762,466 - wcaK OKFHMN_24385 0.36 +1.1 4,762,673 + wcaK OKFHMN_24385 0.52 +0.1 4,762,673 + wcaK OKFHMN_24385 0.52 +0.9 4,762,674 - wcaK OKFHMN_24385 0.52 +1.4 4,762,856 - wcaK OKFHMN_24385 0.66 -2.5 4,762,872 - wcaK OKFHMN_24385 0.67 +0.4 4,762,874 - wcaK OKFHMN_24385 0.67 -3.3 4,762,941 + wcaK OKFHMN_24385 0.73 -2.0 4,762,941 + wcaK OKFHMN_24385 0.73 +0.3 4,762,941 + wcaK OKFHMN_24385 0.73 -1.6 4,762,942 - wcaK OKFHMN_24385 0.73 +0.6 4,763,069 + wcaK OKFHMN_24385 0.83 -0.4 4,763,139 + wcaK OKFHMN_24385 0.88 -1.2 4,763,368 - -0.2 4,763,440 + wcaL OKFHMN_24390 0.13 -1.1 4,763,777 - wcaL OKFHMN_24390 0.40 +0.5 4,763,962 - wcaL OKFHMN_24390 0.55 -0.5 4,763,962 - wcaL OKFHMN_24390 0.55 +0.1 4,764,109 + wcaL OKFHMN_24390 0.67 -0.9 4,764,286 - wcaL OKFHMN_24390 0.82 -1.3 4,764,504 - -1.3 4,764,517 + -2.7 4,764,939 - wcaM OKFHMN_24395 0.30 +0.5 4,765,084 - wcaM OKFHMN_24395 0.41 -0.5 4,765,168 + wcaM OKFHMN_24395 0.47 +0.2 4,765,169 - wcaM OKFHMN_24395 0.47 -2.7 4,765,229 - wcaM OKFHMN_24395 0.51 -0.4 4,765,409 + wcaM OKFHMN_24395 0.64 -0.5
Or see this region's nucleotide sequence