Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hyfD and hyfC are separated by 16 nucleotides hyfC and hyfB are separated by 10 nucleotides
OKFHMN_21885: hyfD - hydrogenase 4 subunit HyfD, at 4,252,234 to 4,253,673
hyfD
OKFHMN_21890: hyfC - hydrogenase 4 subunit HyfC, at 4,253,690 to 4,254,637
hyfC
OKFHMN_21895: hyfB - hydrogenase 4 subunit B, at 4,254,648 to 4,256,666
hyfB
Position (kb) 4253
4254
4255 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 4253.054 kb on + strand, within hyfD at 4253.055 kb on - strand, within hyfD at 4253.136 kb on - strand, within hyfD at 4253.136 kb on - strand, within hyfD at 4253.163 kb on + strand, within hyfD at 4253.163 kb on + strand, within hyfD at 4253.164 kb on - strand, within hyfD at 4253.176 kb on + strand, within hyfD at 4253.208 kb on - strand, within hyfD at 4253.211 kb on - strand, within hyfD at 4253.285 kb on - strand, within hyfD at 4253.647 kb on + strand at 4253.840 kb on - strand, within hyfC at 4254.006 kb on - strand, within hyfC at 4254.089 kb on + strand, within hyfC at 4254.127 kb on + strand, within hyfC at 4254.309 kb on + strand, within hyfC at 4254.310 kb on - strand, within hyfC at 4254.310 kb on - strand, within hyfC at 4254.310 kb on - strand, within hyfC at 4254.358 kb on + strand, within hyfC at 4254.358 kb on + strand, within hyfC at 4254.440 kb on + strand, within hyfC at 4254.442 kb on + strand, within hyfC at 4254.443 kb on - strand, within hyfC at 4254.523 kb on + strand, within hyfC at 4254.533 kb on + strand, within hyfC at 4254.612 kb on + strand at 4254.660 kb on - strand at 4254.695 kb on - strand at 4254.703 kb on + strand at 4254.703 kb on + strand at 4254.858 kb on - strand, within hyfB at 4255.054 kb on - strand, within hyfB at 4255.056 kb on + strand, within hyfB at 4255.056 kb on + strand, within hyfB at 4255.069 kb on - strand, within hyfB at 4255.105 kb on - strand, within hyfB at 4255.149 kb on - strand, within hyfB at 4255.170 kb on + strand, within hyfB at 4255.196 kb on + strand, within hyfB at 4255.197 kb on - strand, within hyfB at 4255.197 kb on - strand, within hyfB at 4255.231 kb on - strand, within hyfB at 4255.308 kb on - strand, within hyfB at 4255.316 kb on - strand, within hyfB at 4255.335 kb on + strand, within hyfB at 4255.371 kb on - strand, within hyfB at 4255.393 kb on - strand, within hyfB at 4255.455 kb on - strand, within hyfB at 4255.580 kb on + strand, within hyfB
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 4,253,054 + hyfD OKFHMN_21885 0.57 -0.3 4,253,055 - hyfD OKFHMN_21885 0.57 -2.0 4,253,136 - hyfD OKFHMN_21885 0.63 -1.1 4,253,136 - hyfD OKFHMN_21885 0.63 +1.2 4,253,163 + hyfD OKFHMN_21885 0.65 +0.8 4,253,163 + hyfD OKFHMN_21885 0.65 +0.2 4,253,164 - hyfD OKFHMN_21885 0.65 -0.4 4,253,176 + hyfD OKFHMN_21885 0.65 -0.1 4,253,208 - hyfD OKFHMN_21885 0.68 -0.5 4,253,211 - hyfD OKFHMN_21885 0.68 +0.8 4,253,285 - hyfD OKFHMN_21885 0.73 -0.5 4,253,647 + -2.2 4,253,840 - hyfC OKFHMN_21890 0.16 -0.2 4,254,006 - hyfC OKFHMN_21890 0.33 +0.1 4,254,089 + hyfC OKFHMN_21890 0.42 -0.7 4,254,127 + hyfC OKFHMN_21890 0.46 -0.4 4,254,309 + hyfC OKFHMN_21890 0.65 +1.3 4,254,310 - hyfC OKFHMN_21890 0.65 +0.4 4,254,310 - hyfC OKFHMN_21890 0.65 +3.2 4,254,310 - hyfC OKFHMN_21890 0.65 +0.4 4,254,358 + hyfC OKFHMN_21890 0.70 +0.4 4,254,358 + hyfC OKFHMN_21890 0.70 -0.6 4,254,440 + hyfC OKFHMN_21890 0.79 -0.0 4,254,442 + hyfC OKFHMN_21890 0.79 +0.5 4,254,443 - hyfC OKFHMN_21890 0.79 -0.8 4,254,523 + hyfC OKFHMN_21890 0.88 +0.4 4,254,533 + hyfC OKFHMN_21890 0.89 -0.5 4,254,612 + +0.1 4,254,660 - -0.1 4,254,695 - +0.2 4,254,703 + +0.5 4,254,703 + -1.2 4,254,858 - hyfB OKFHMN_21895 0.10 -0.0 4,255,054 - hyfB OKFHMN_21895 0.20 -2.7 4,255,056 + hyfB OKFHMN_21895 0.20 +2.0 4,255,056 + hyfB OKFHMN_21895 0.20 -0.0 4,255,069 - hyfB OKFHMN_21895 0.21 +0.7 4,255,105 - hyfB OKFHMN_21895 0.23 -0.2 4,255,149 - hyfB OKFHMN_21895 0.25 +1.0 4,255,170 + hyfB OKFHMN_21895 0.26 +0.2 4,255,196 + hyfB OKFHMN_21895 0.27 +0.3 4,255,197 - hyfB OKFHMN_21895 0.27 -0.3 4,255,197 - hyfB OKFHMN_21895 0.27 -0.5 4,255,231 - hyfB OKFHMN_21895 0.29 -0.3 4,255,308 - hyfB OKFHMN_21895 0.33 +0.5 4,255,316 - hyfB OKFHMN_21895 0.33 +0.5 4,255,335 + hyfB OKFHMN_21895 0.34 +0.4 4,255,371 - hyfB OKFHMN_21895 0.36 -0.7 4,255,393 - hyfB OKFHMN_21895 0.37 +0.5 4,255,455 - hyfB OKFHMN_21895 0.40 -3.7 4,255,580 + hyfB OKFHMN_21895 0.46 +1.7
Or see this region's nucleotide sequence