Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hyfR and hyfJ are separated by 29 nucleotides hyfJ and hyfI overlap by 8 nucleotides hyfI and hyfH overlap by 4 nucleotides
OKFHMN_21850: hyfR - DNA-binding transcriptional regulator HyfR, at 4,244,524 to 4,246,536
hyfR
OKFHMN_21855: hyfJ - hydrogenase 4 assembly chaperone HyfJ, at 4,246,566 to 4,246,979
hyfJ
OKFHMN_21860: hyfI - hydrogenase 4 catalytic subunit HyfI, at 4,246,972 to 4,247,730
hyfI
OKFHMN_21865: hyfH - hydrogenase 4 subunit H, at 4,247,727 to 4,248,272
hyfH
Position (kb) 4246
4247 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4245.582 kb on + strand, within hyfR at 4245.583 kb on - strand, within hyfR at 4245.637 kb on + strand, within hyfR at 4245.638 kb on - strand, within hyfR at 4245.638 kb on - strand, within hyfR at 4245.638 kb on - strand, within hyfR at 4245.641 kb on - strand, within hyfR at 4245.641 kb on - strand, within hyfR at 4245.672 kb on + strand, within hyfR at 4245.679 kb on - strand, within hyfR at 4245.705 kb on + strand, within hyfR at 4245.705 kb on + strand, within hyfR at 4245.705 kb on + strand, within hyfR at 4245.706 kb on - strand, within hyfR at 4245.763 kb on + strand, within hyfR at 4245.763 kb on + strand, within hyfR at 4245.763 kb on + strand, within hyfR at 4245.769 kb on + strand, within hyfR at 4245.769 kb on + strand, within hyfR at 4245.963 kb on + strand, within hyfR at 4245.963 kb on + strand, within hyfR at 4246.232 kb on - strand, within hyfR at 4246.346 kb on + strand at 4246.346 kb on + strand at 4246.346 kb on + strand at 4246.347 kb on - strand at 4246.377 kb on + strand at 4246.377 kb on + strand at 4246.548 kb on - strand at 4246.591 kb on + strand at 4246.596 kb on - strand at 4246.643 kb on + strand, within hyfJ at 4246.725 kb on - strand, within hyfJ at 4246.760 kb on + strand, within hyfJ at 4246.882 kb on + strand, within hyfJ at 4246.882 kb on + strand, within hyfJ at 4246.882 kb on + strand, within hyfJ at 4246.888 kb on - strand, within hyfJ at 4247.019 kb on + strand at 4247.019 kb on + strand at 4247.073 kb on + strand, within hyfI at 4247.087 kb on - strand, within hyfI at 4247.206 kb on - strand, within hyfI at 4247.445 kb on - strand, within hyfI at 4247.445 kb on - strand, within hyfI at 4247.463 kb on + strand, within hyfI at 4247.494 kb on + strand, within hyfI at 4247.495 kb on - strand, within hyfI at 4247.532 kb on + strand, within hyfI at 4247.532 kb on + strand, within hyfI at 4247.533 kb on - strand, within hyfI at 4247.644 kb on - strand, within hyfI at 4247.715 kb on + strand at 4247.715 kb on + strand at 4247.731 kb on - strand at 4247.731 kb on - strand at 4247.731 kb on - strand at 4247.762 kb on + strand at 4247.932 kb on - strand, within hyfH at 4247.953 kb on + strand, within hyfH at 4247.967 kb on - strand, within hyfH
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 4,245,582 + hyfR OKFHMN_21850 0.53 +0.0 4,245,583 - hyfR OKFHMN_21850 0.53 +0.4 4,245,637 + hyfR OKFHMN_21850 0.55 +0.6 4,245,638 - hyfR OKFHMN_21850 0.55 -0.3 4,245,638 - hyfR OKFHMN_21850 0.55 -2.1 4,245,638 - hyfR OKFHMN_21850 0.55 -0.2 4,245,641 - hyfR OKFHMN_21850 0.55 +0.1 4,245,641 - hyfR OKFHMN_21850 0.55 -0.0 4,245,672 + hyfR OKFHMN_21850 0.57 -0.9 4,245,679 - hyfR OKFHMN_21850 0.57 +1.5 4,245,705 + hyfR OKFHMN_21850 0.59 +0.4 4,245,705 + hyfR OKFHMN_21850 0.59 -0.4 4,245,705 + hyfR OKFHMN_21850 0.59 -0.1 4,245,706 - hyfR OKFHMN_21850 0.59 -0.0 4,245,763 + hyfR OKFHMN_21850 0.62 +0.6 4,245,763 + hyfR OKFHMN_21850 0.62 -0.1 4,245,763 + hyfR OKFHMN_21850 0.62 -0.2 4,245,769 + hyfR OKFHMN_21850 0.62 -0.6 4,245,769 + hyfR OKFHMN_21850 0.62 -0.4 4,245,963 + hyfR OKFHMN_21850 0.71 -0.8 4,245,963 + hyfR OKFHMN_21850 0.71 +0.9 4,246,232 - hyfR OKFHMN_21850 0.85 -0.3 4,246,346 + -0.6 4,246,346 + +1.5 4,246,346 + +0.5 4,246,347 - -0.5 4,246,377 + +0.6 4,246,377 + +1.6 4,246,548 - -0.2 4,246,591 + -1.0 4,246,596 - -1.4 4,246,643 + hyfJ OKFHMN_21855 0.19 +0.1 4,246,725 - hyfJ OKFHMN_21855 0.38 +0.5 4,246,760 + hyfJ OKFHMN_21855 0.47 -0.7 4,246,882 + hyfJ OKFHMN_21855 0.76 +1.0 4,246,882 + hyfJ OKFHMN_21855 0.76 -0.2 4,246,882 + hyfJ OKFHMN_21855 0.76 +0.9 4,246,888 - hyfJ OKFHMN_21855 0.78 +1.0 4,247,019 + -0.2 4,247,019 + +0.1 4,247,073 + hyfI OKFHMN_21860 0.13 -0.1 4,247,087 - hyfI OKFHMN_21860 0.15 -1.6 4,247,206 - hyfI OKFHMN_21860 0.31 -0.6 4,247,445 - hyfI OKFHMN_21860 0.62 +0.3 4,247,445 - hyfI OKFHMN_21860 0.62 +0.8 4,247,463 + hyfI OKFHMN_21860 0.65 +0.5 4,247,494 + hyfI OKFHMN_21860 0.69 -1.1 4,247,495 - hyfI OKFHMN_21860 0.69 +0.1 4,247,532 + hyfI OKFHMN_21860 0.74 -0.3 4,247,532 + hyfI OKFHMN_21860 0.74 +0.5 4,247,533 - hyfI OKFHMN_21860 0.74 -0.5 4,247,644 - hyfI OKFHMN_21860 0.89 +0.8 4,247,715 + +0.5 4,247,715 + +1.0 4,247,731 - +0.2 4,247,731 - -2.0 4,247,731 - +0.4 4,247,762 + +0.6 4,247,932 - hyfH OKFHMN_21865 0.38 -2.8 4,247,953 + hyfH OKFHMN_21865 0.41 +0.1 4,247,967 - hyfH OKFHMN_21865 0.44 -1.0
Or see this region's nucleotide sequence