Strain Fitness in Escherichia coli ECRC100 around OKFHMN_21840

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntbepA and perM are separated by 212 nucleotidesperM and focB are separated by 37 nucleotidesfocB and hyfR are separated by 21 nucleotides OKFHMN_21835: bepA - beta-barrel assembly-enhancing protease, at 4,240,879 to 4,242,342 bepA OKFHMN_21840: perM - Putative permease PerM, at 4,242,555 to 4,243,616 perM OKFHMN_21845: focB - formate transporter FocB, at 4,243,654 to 4,244,502 focB OKFHMN_21850: hyfR - DNA-binding transcriptional regulator HyfR, at 4,244,524 to 4,246,536 hyfR Position (kb) 4242 4243 4244Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4241.563 kb on - strand, within bepAat 4242.119 kb on + strand, within bepAat 4242.120 kb on - strand, within bepAat 4242.176 kb on + strand, within bepAat 4242.176 kb on + strand, within bepAat 4242.179 kb on + strand, within bepAat 4242.180 kb on - strand, within bepAat 4242.180 kb on - strand, within bepAat 4242.180 kb on - strand, within bepAat 4242.342 kb on + strandat 4242.342 kb on + strandat 4242.343 kb on - strandat 4242.343 kb on - strandat 4242.368 kb on + strandat 4242.369 kb on - strandat 4242.422 kb on + strandat 4242.423 kb on - strandat 4242.507 kb on + strandat 4242.555 kb on - strandat 4242.555 kb on - strandat 4242.689 kb on + strand, within perMat 4242.690 kb on - strand, within perMat 4242.738 kb on - strand, within perMat 4242.791 kb on + strand, within perMat 4242.793 kb on + strand, within perMat 4242.793 kb on + strand, within perMat 4242.794 kb on - strand, within perMat 4242.846 kb on + strand, within perMat 4242.850 kb on + strand, within perMat 4243.061 kb on - strand, within perMat 4243.188 kb on + strand, within perMat 4243.189 kb on - strand, within perMat 4243.189 kb on - strand, within perMat 4243.219 kb on + strand, within perMat 4243.307 kb on + strand, within perMat 4243.307 kb on + strand, within perMat 4243.314 kb on + strand, within perMat 4243.670 kb on - strandat 4243.678 kb on - strandat 4243.693 kb on - strandat 4243.693 kb on - strandat 4243.693 kb on - strandat 4243.720 kb on - strandat 4243.753 kb on - strand, within focBat 4243.755 kb on + strand, within focBat 4243.755 kb on + strand, within focBat 4243.999 kb on - strand, within focBat 4244.028 kb on + strand, within focBat 4244.134 kb on - strand, within focBat 4244.170 kb on - strand, within focBat 4244.371 kb on - strand, within focBat 4244.526 kb on - strandat 4244.526 kb on - strandat 4244.526 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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4,241,563 - bepA OKFHMN_21835 0.47 -0.1
4,242,119 + bepA OKFHMN_21835 0.85 +1.0
4,242,120 - bepA OKFHMN_21835 0.85 -0.8
4,242,176 + bepA OKFHMN_21835 0.89 -0.9
4,242,176 + bepA OKFHMN_21835 0.89 +0.3
4,242,179 + bepA OKFHMN_21835 0.89 -1.2
4,242,180 - bepA OKFHMN_21835 0.89 -1.4
4,242,180 - bepA OKFHMN_21835 0.89 -0.6
4,242,180 - bepA OKFHMN_21835 0.89 +0.6
4,242,342 + +0.7
4,242,342 + +0.8
4,242,343 - -2.0
4,242,343 - +0.0
4,242,368 + +1.5
4,242,369 - +0.0
4,242,422 + +0.7
4,242,423 - +0.4
4,242,507 + +0.1
4,242,555 - +1.9
4,242,555 - -1.9
4,242,689 + perM OKFHMN_21840 0.13 +1.3
4,242,690 - perM OKFHMN_21840 0.13 -0.6
4,242,738 - perM OKFHMN_21840 0.17 +1.2
4,242,791 + perM OKFHMN_21840 0.22 +0.2
4,242,793 + perM OKFHMN_21840 0.22 +0.6
4,242,793 + perM OKFHMN_21840 0.22 +1.0
4,242,794 - perM OKFHMN_21840 0.23 +0.9
4,242,846 + perM OKFHMN_21840 0.27 +0.2
4,242,850 + perM OKFHMN_21840 0.28 +1.0
4,243,061 - perM OKFHMN_21840 0.48 +0.7
4,243,188 + perM OKFHMN_21840 0.60 -0.3
4,243,189 - perM OKFHMN_21840 0.60 -0.5
4,243,189 - perM OKFHMN_21840 0.60 -2.0
4,243,219 + perM OKFHMN_21840 0.63 +0.4
4,243,307 + perM OKFHMN_21840 0.71 -3.0
4,243,307 + perM OKFHMN_21840 0.71 -1.1
4,243,314 + perM OKFHMN_21840 0.71 +0.2
4,243,670 - +0.1
4,243,678 - +0.2
4,243,693 - -0.4
4,243,693 - +0.3
4,243,693 - -0.2
4,243,720 - -0.0
4,243,753 - focB OKFHMN_21845 0.12 +0.4
4,243,755 + focB OKFHMN_21845 0.12 -0.1
4,243,755 + focB OKFHMN_21845 0.12 -0.8
4,243,999 - focB OKFHMN_21845 0.41 -0.8
4,244,028 + focB OKFHMN_21845 0.44 -1.9
4,244,134 - focB OKFHMN_21845 0.57 -3.3
4,244,170 - focB OKFHMN_21845 0.61 +0.5
4,244,371 - focB OKFHMN_21845 0.84 +0.3
4,244,526 - -0.2
4,244,526 - +0.0
4,244,526 - -0.2

Or see this region's nucleotide sequence