Strain Fitness in Escherichia coli ECRC100 around OKFHMN_18005

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmlaC and mlaC overlap by 1 nucleotidesmlaC and ibaG are separated by 144 nucleotidesibaG and murA are separated by 54 nucleotidesmurA and sfsB are separated by 47 nucleotidessfsB and ispB are separated by 228 nucleotides OKFHMN_17990: mlaC - phospholipid-binding protein MlaC, at 3,496,152 to 3,496,787 mlaC OKFHMN_17995: mlaC - phospholipid-binding protein MlaC, at 3,496,787 to 3,497,080 mlaC OKFHMN_18000: ibaG - BolA family iron metabolism protein IbaG, at 3,497,225 to 3,497,494 ibaG OKFHMN_18005: murA - UDP-N-acetylglucosamine 1-carboxyvinyltransferase, at 3,497,549 to 3,498,808 murA OKFHMN_18010: sfsB - DNA-binding transcriptional regulator SfsB, at 3,498,856 to 3,499,134 sfsB OKFHMN_18015: ispB - octaprenyl diphosphate synthase, at 3,499,363 to 3,500,334 ispB Position (kb) 3497 3498 3499Strain fitness (log2 ratio) -3 -2 -1 0 1at 3496.563 kb on - strand, within mlaCat 3496.641 kb on + strand, within mlaCat 3496.642 kb on - strand, within mlaCat 3496.642 kb on - strand, within mlaCat 3496.642 kb on - strand, within mlaCat 3496.668 kb on - strand, within mlaCat 3496.705 kb on + strand, within mlaCat 3496.706 kb on - strand, within mlaCat 3496.706 kb on - strand, within mlaCat 3496.709 kb on + strand, within mlaCat 3496.710 kb on - strand, within mlaCat 3496.904 kb on - strand, within mlaCat 3496.904 kb on - strand, within mlaCat 3496.904 kb on - strand, within mlaCat 3497.030 kb on + strand, within mlaCat 3497.031 kb on - strand, within mlaCat 3497.031 kb on - strand, within mlaCat 3497.032 kb on + strand, within mlaCat 3497.173 kb on + strandat 3497.174 kb on - strandat 3497.177 kb on + strandat 3497.405 kb on + strand, within ibaGat 3497.407 kb on + strand, within ibaGat 3497.407 kb on + strand, within ibaGat 3497.447 kb on + strand, within ibaGat 3497.449 kb on + strand, within ibaGat 3498.870 kb on + strandat 3498.875 kb on + strandat 3498.876 kb on - strandat 3498.929 kb on + strand, within sfsBat 3498.946 kb on - strand, within sfsBat 3498.946 kb on - strand, within sfsBat 3498.946 kb on - strand, within sfsBat 3498.979 kb on - strand, within sfsBat 3498.979 kb on - strand, within sfsBat 3499.126 kb on - strandat 3499.126 kb on - strandat 3499.149 kb on + strandat 3499.149 kb on + strandat 3499.195 kb on + strandat 3499.233 kb on - strandat 3499.257 kb on - strandat 3499.266 kb on + strandat 3499.267 kb on - strandat 3499.267 kb on - strandat 3499.267 kb on - strandat 3499.289 kb on + strandat 3499.306 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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3,496,563 - mlaC OKFHMN_17990 0.65 -0.3
3,496,641 + mlaC OKFHMN_17990 0.77 +0.8
3,496,642 - mlaC OKFHMN_17990 0.77 -0.6
3,496,642 - mlaC OKFHMN_17990 0.77 +0.1
3,496,642 - mlaC OKFHMN_17990 0.77 +0.6
3,496,668 - mlaC OKFHMN_17990 0.81 +0.1
3,496,705 + mlaC OKFHMN_17990 0.87 +0.6
3,496,706 - mlaC OKFHMN_17990 0.87 +0.5
3,496,706 - mlaC OKFHMN_17990 0.87 -0.0
3,496,709 + mlaC OKFHMN_17990 0.88 -0.3
3,496,710 - mlaC OKFHMN_17990 0.88 -0.0
3,496,904 - mlaC OKFHMN_17995 0.40 +0.6
3,496,904 - mlaC OKFHMN_17995 0.40 +0.4
3,496,904 - mlaC OKFHMN_17995 0.40 +0.5
3,497,030 + mlaC OKFHMN_17995 0.83 +0.2
3,497,031 - mlaC OKFHMN_17995 0.83 +0.1
3,497,031 - mlaC OKFHMN_17995 0.83 +0.3
3,497,032 + mlaC OKFHMN_17995 0.83 -0.3
3,497,173 + +0.6
3,497,174 - +0.4
3,497,177 + -0.3
3,497,405 + ibaG OKFHMN_18000 0.67 -0.6
3,497,407 + ibaG OKFHMN_18000 0.67 -0.6
3,497,407 + ibaG OKFHMN_18000 0.67 -0.1
3,497,447 + ibaG OKFHMN_18000 0.82 -2.9
3,497,449 + ibaG OKFHMN_18000 0.83 +0.0
3,498,870 + +0.2
3,498,875 + -1.0
3,498,876 - +1.0
3,498,929 + sfsB OKFHMN_18010 0.26 -0.5
3,498,946 - sfsB OKFHMN_18010 0.32 -0.4
3,498,946 - sfsB OKFHMN_18010 0.32 -0.3
3,498,946 - sfsB OKFHMN_18010 0.32 +0.0
3,498,979 - sfsB OKFHMN_18010 0.44 +0.6
3,498,979 - sfsB OKFHMN_18010 0.44 -0.4
3,499,126 - +0.5
3,499,126 - -0.3
3,499,149 + -0.2
3,499,149 + +0.4
3,499,195 + +0.4
3,499,233 - +1.0
3,499,257 - +0.1
3,499,266 + -0.7
3,499,267 - -0.1
3,499,267 - -0.5
3,499,267 - -1.0
3,499,289 + +0.9
3,499,306 - +0.1

Or see this region's nucleotide sequence