Strain Fitness in Escherichia coli ECRC100 around OKFHMN_16025

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntghrB and yiaD are separated by 103 nucleotidesyiaD and bisC are separated by 152 nucleotides OKFHMN_16020: ghrB - glyoxylate/hydroxypyruvate reductase GhrB, at 3,109,215 to 3,110,189 ghrB OKFHMN_16025: yiaD - OmpA family lipoprotein, at 3,110,293 to 3,110,952 yiaD OKFHMN_16030: bisC - biotin sulfoxide reductase, at 3,111,105 to 3,113,438 bisC Position (kb) 3110 3111Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3109.381 kb on - strand, within ghrBat 3109.491 kb on - strand, within ghrBat 3109.596 kb on + strand, within ghrBat 3109.597 kb on - strand, within ghrBat 3109.649 kb on + strand, within ghrBat 3109.669 kb on + strand, within ghrBat 3109.670 kb on - strand, within ghrBat 3109.893 kb on + strand, within ghrBat 3109.961 kb on - strand, within ghrBat 3110.146 kb on - strandat 3110.209 kb on + strandat 3110.209 kb on + strandat 3110.210 kb on - strandat 3110.294 kb on + strandat 3110.305 kb on + strandat 3110.337 kb on + strandat 3110.391 kb on + strand, within yiaDat 3110.499 kb on + strand, within yiaDat 3110.499 kb on + strand, within yiaDat 3110.640 kb on + strand, within yiaDat 3110.661 kb on + strand, within yiaDat 3110.714 kb on + strand, within yiaDat 3110.714 kb on + strand, within yiaDat 3110.714 kb on + strand, within yiaDat 3110.714 kb on + strand, within yiaDat 3110.715 kb on - strand, within yiaDat 3110.715 kb on - strand, within yiaDat 3110.715 kb on - strand, within yiaDat 3110.715 kb on - strand, within yiaDat 3110.876 kb on + strand, within yiaDat 3110.877 kb on - strand, within yiaDat 3110.884 kb on - strand, within yiaDat 3110.884 kb on - strand, within yiaDat 3110.932 kb on - strandat 3110.937 kb on - strandat 3110.956 kb on + strandat 3110.975 kb on + strandat 3111.007 kb on + strandat 3111.007 kb on + strandat 3111.029 kb on + strandat 3111.030 kb on - strandat 3111.042 kb on - strandat 3111.060 kb on - strandat 3111.067 kb on - strandat 3111.077 kb on + strandat 3111.078 kb on - strandat 3111.083 kb on + strandat 3111.083 kb on + strandat 3111.084 kb on - strandat 3111.192 kb on - strandat 3111.384 kb on - strand, within bisCat 3111.399 kb on - strand, within bisCat 3111.418 kb on - strand, within bisCat 3111.418 kb on - strand, within bisCat 3111.418 kb on - strand, within bisCat 3111.490 kb on + strand, within bisCat 3111.491 kb on - strand, within bisCat 3111.491 kb on - strand, within bisCat 3111.493 kb on + strand, within bisCat 3111.493 kb on + strand, within bisCat 3111.494 kb on - strand, within bisCat 3111.501 kb on + strand, within bisCat 3111.501 kb on + strand, within bisCat 3111.501 kb on + strand, within bisCat 3111.502 kb on - strand, within bisCat 3111.502 kb on - strand, within bisCat 3111.502 kb on - strand, within bisCat 3111.504 kb on - strand, within bisCat 3111.504 kb on - strand, within bisCat 3111.576 kb on + strand, within bisCat 3111.601 kb on - strand, within bisCat 3111.639 kb on - strand, within bisCat 3111.945 kb on + strand, within bisCat 3111.946 kb on - strand, within bisC

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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3,109,381 - ghrB OKFHMN_16020 0.17 -1.3
3,109,491 - ghrB OKFHMN_16020 0.28 -0.9
3,109,596 + ghrB OKFHMN_16020 0.39 +1.0
3,109,597 - ghrB OKFHMN_16020 0.39 -0.1
3,109,649 + ghrB OKFHMN_16020 0.45 +0.4
3,109,669 + ghrB OKFHMN_16020 0.47 -0.1
3,109,670 - ghrB OKFHMN_16020 0.47 +0.6
3,109,893 + ghrB OKFHMN_16020 0.70 -0.0
3,109,961 - ghrB OKFHMN_16020 0.77 +0.0
3,110,146 - +0.7
3,110,209 + -0.7
3,110,209 + +0.5
3,110,210 - -1.4
3,110,294 + -0.6
3,110,305 + -0.2
3,110,337 + -0.7
3,110,391 + yiaD OKFHMN_16025 0.15 +0.5
3,110,499 + yiaD OKFHMN_16025 0.31 +0.0
3,110,499 + yiaD OKFHMN_16025 0.31 -0.1
3,110,640 + yiaD OKFHMN_16025 0.53 -0.5
3,110,661 + yiaD OKFHMN_16025 0.56 -0.2
3,110,714 + yiaD OKFHMN_16025 0.64 +0.1
3,110,714 + yiaD OKFHMN_16025 0.64 -0.5
3,110,714 + yiaD OKFHMN_16025 0.64 -0.2
3,110,714 + yiaD OKFHMN_16025 0.64 +0.1
3,110,715 - yiaD OKFHMN_16025 0.64 -0.2
3,110,715 - yiaD OKFHMN_16025 0.64 -0.7
3,110,715 - yiaD OKFHMN_16025 0.64 -0.3
3,110,715 - yiaD OKFHMN_16025 0.64 +0.5
3,110,876 + yiaD OKFHMN_16025 0.88 -0.3
3,110,877 - yiaD OKFHMN_16025 0.88 +0.3
3,110,884 - yiaD OKFHMN_16025 0.90 -0.9
3,110,884 - yiaD OKFHMN_16025 0.90 -0.7
3,110,932 - -1.1
3,110,937 - +1.8
3,110,956 + -1.7
3,110,975 + -0.6
3,111,007 + -0.2
3,111,007 + -0.5
3,111,029 + +0.0
3,111,030 - -1.2
3,111,042 - -1.0
3,111,060 - +0.1
3,111,067 - +0.1
3,111,077 + +1.6
3,111,078 - +2.6
3,111,083 + +0.1
3,111,083 + -0.1
3,111,084 - -0.0
3,111,192 - -0.4
3,111,384 - bisC OKFHMN_16030 0.12 +0.2
3,111,399 - bisC OKFHMN_16030 0.13 +0.1
3,111,418 - bisC OKFHMN_16030 0.13 +0.3
3,111,418 - bisC OKFHMN_16030 0.13 -0.0
3,111,418 - bisC OKFHMN_16030 0.13 +0.7
3,111,490 + bisC OKFHMN_16030 0.16 -0.3
3,111,491 - bisC OKFHMN_16030 0.17 -0.2
3,111,491 - bisC OKFHMN_16030 0.17 +0.3
3,111,493 + bisC OKFHMN_16030 0.17 -0.7
3,111,493 + bisC OKFHMN_16030 0.17 -0.9
3,111,494 - bisC OKFHMN_16030 0.17 +0.2
3,111,501 + bisC OKFHMN_16030 0.17 +0.0
3,111,501 + bisC OKFHMN_16030 0.17 -0.2
3,111,501 + bisC OKFHMN_16030 0.17 -0.2
3,111,502 - bisC OKFHMN_16030 0.17 -3.5
3,111,502 - bisC OKFHMN_16030 0.17 -0.1
3,111,502 - bisC OKFHMN_16030 0.17 +1.0
3,111,504 - bisC OKFHMN_16030 0.17 +0.5
3,111,504 - bisC OKFHMN_16030 0.17 +0.5
3,111,576 + bisC OKFHMN_16030 0.20 +0.2
3,111,601 - bisC OKFHMN_16030 0.21 +0.6
3,111,639 - bisC OKFHMN_16030 0.23 +0.1
3,111,945 + bisC OKFHMN_16030 0.36 -0.1
3,111,946 - bisC OKFHMN_16030 0.36 -0.2

Or see this region's nucleotide sequence