Strain Fitness in Escherichia coli ECRC100 around OKFHMN_15680

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrfaJ and rfaB are separated by 8 nucleotidesrfaB and rfaL are separated by 35 nucleotides OKFHMN_15675: rfaJ - glucosyltransferase, at 3,027,870 to 3,028,883 rfaJ OKFHMN_15680: rfaB - UDP-glucose--(glucosyl)LPS alpha-1,2-glucosyltransferase, at 3,028,892 to 3,030,034 rfaB OKFHMN_15685: rfaL - O-antigen ligase RfaL, at 3,030,070 to 3,031,278 rfaL Position (kb) 3028 3029 3030 3031Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1 2at 3028.133 kb on - strand, within rfaJat 3028.401 kb on + strand, within rfaJat 3028.669 kb on - strand, within rfaJat 3028.864 kb on - strandat 3028.974 kb on - strandat 3029.212 kb on + strand, within rfaBat 3029.671 kb on + strand, within rfaBat 3029.782 kb on + strand, within rfaBat 3029.795 kb on - strand, within rfaBat 3030.274 kb on + strand, within rfaLat 3030.331 kb on + strand, within rfaLat 3030.376 kb on - strand, within rfaLat 3030.392 kb on + strand, within rfaLat 3030.463 kb on - strand, within rfaLat 3030.795 kb on + strand, within rfaLat 3030.795 kb on + strand, within rfaLat 3030.934 kb on + strand, within rfaLat 3030.935 kb on - strand, within rfaLat 3030.958 kb on + strand, within rfaLat 3030.959 kb on - strand, within rfaLat 3030.962 kb on - strand, within rfaL

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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3,028,133 - rfaJ OKFHMN_15675 0.26 +0.6
3,028,401 + rfaJ OKFHMN_15675 0.52 +0.8
3,028,669 - rfaJ OKFHMN_15675 0.79 -1.8
3,028,864 - +1.1
3,028,974 - -0.8
3,029,212 + rfaB OKFHMN_15680 0.28 +1.3
3,029,671 + rfaB OKFHMN_15680 0.68 +0.9
3,029,782 + rfaB OKFHMN_15680 0.78 +0.3
3,029,795 - rfaB OKFHMN_15680 0.79 +1.4
3,030,274 + rfaL OKFHMN_15685 0.17 +0.6
3,030,331 + rfaL OKFHMN_15685 0.22 -2.2
3,030,376 - rfaL OKFHMN_15685 0.25 +0.9
3,030,392 + rfaL OKFHMN_15685 0.27 +1.1
3,030,463 - rfaL OKFHMN_15685 0.33 +0.1
3,030,795 + rfaL OKFHMN_15685 0.60 +0.8
3,030,795 + rfaL OKFHMN_15685 0.60 +1.4
3,030,934 + rfaL OKFHMN_15685 0.71 +1.5
3,030,935 - rfaL OKFHMN_15685 0.72 +1.8
3,030,958 + rfaL OKFHMN_15685 0.73 +1.6
3,030,959 - rfaL OKFHMN_15685 0.74 +0.7
3,030,962 - rfaL OKFHMN_15685 0.74 -6.5

Or see this region's nucleotide sequence