Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sepD and escJ are separated by 5 nucleotides escJ and escI are separated by 2 nucleotides escI and espZ are separated by 83 nucleotides espZ and cesL are separated by 184 nucleotides cesL and escV overlap by 4 nucleotides
OKFHMN_15335: sepD - type III secretion system LEE switch protein SepD, at 2,963,703 to 2,964,158
sepD
OKFHMN_15340: escJ - type III secretion system LEE inner membrane ring protein EscJ, at 2,964,164 to 2,964,736
escJ
OKFHMN_15345: escI - Type III secretion system inner rod component EscI, at 2,964,739 to 2,965,116
escI
OKFHMN_15350: espZ - type III secretion system LEE cytoprotective effector EspZ, at 2,965,200 to 2,965,499
espZ
OKFHMN_15355: cesL - type III secretion system LEE chaperone CesL, at 2,965,684 to 2,966,037
cesL
OKFHMN_15360: escV - type III secretion system LEE export apparatus protein EscV, at 2,966,034 to 2,968,061
escV
Position (kb)
2964
2965
2966 Strain fitness (log2 ratio)
-1
0
1 at 2963.775 kb on + strand, within sepD at 2964.096 kb on + strand, within sepD at 2964.139 kb on - strand at 2964.158 kb on + strand at 2964.272 kb on + strand, within escJ at 2964.721 kb on + strand at 2964.878 kb on - strand, within escI at 2964.878 kb on - strand, within escI at 2964.878 kb on - strand, within escI at 2964.982 kb on + strand, within escI at 2965.038 kb on + strand, within escI at 2965.160 kb on - strand at 2965.239 kb on - strand, within espZ at 2965.248 kb on + strand, within espZ at 2965.279 kb on + strand, within espZ at 2965.322 kb on + strand, within espZ at 2965.368 kb on - strand, within espZ at 2965.540 kb on + strand at 2965.606 kb on + strand at 2965.612 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 2,963,775 + sepD OKFHMN_15335 0.16 +0.2 2,964,096 + sepD OKFHMN_15335 0.86 +0.1 2,964,139 - +1.7 2,964,158 + -0.4 2,964,272 + escJ OKFHMN_15340 0.19 +0.3 2,964,721 + -1.0 2,964,878 - escI OKFHMN_15345 0.37 -0.0 2,964,878 - escI OKFHMN_15345 0.37 +0.3 2,964,878 - escI OKFHMN_15345 0.37 +0.3 2,964,982 + escI OKFHMN_15345 0.64 +1.4 2,965,038 + escI OKFHMN_15345 0.79 -0.6 2,965,160 - -0.0 2,965,239 - espZ OKFHMN_15350 0.13 -0.3 2,965,248 + espZ OKFHMN_15350 0.16 -0.1 2,965,279 + espZ OKFHMN_15350 0.26 -0.8 2,965,322 + espZ OKFHMN_15350 0.41 +0.9 2,965,368 - espZ OKFHMN_15350 0.56 -0.9 2,965,540 + +0.9 2,965,606 + -0.8 2,965,612 + -0.4
Or see this region's nucleotide sequence