Strain Fitness in Escherichia coli ECRC100 around OKFHMN_15325

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgrlR and grlA are separated by 54 nucleotidesgrlA and cesD are separated by 383 nucleotidescesD and escC are separated by 13 nucleotides OKFHMN_15315: grlR - type III secretion system LEE GrlA-binding negative regulator GrlR, at 2,960,473 to 2,960,844 grlR OKFHMN_15320: grlA - type III secretion system LEE transcriptional regulator GrlA, at 2,960,899 to 2,961,312 grlA OKFHMN_15325: cesD - SycD/LcrH family type III secretion system chaperone CesD, at 2,961,696 to 2,962,151 cesD OKFHMN_15330: escC - type III secretion system LEE outer membrane ring protein EscC, at 2,962,165 to 2,963,703 escC Position (kb) 2961 2962 2963Strain fitness (log2 ratio) -1 0 1at 2960.806 kb on - strand, within grlRat 2960.974 kb on - strand, within grlAat 2961.253 kb on - strand, within grlAat 2961.271 kb on - strandat 2961.468 kb on + strandat 2961.484 kb on + strandat 2961.726 kb on - strandat 2961.761 kb on + strand, within cesDat 2961.823 kb on + strand, within cesDat 2962.024 kb on + strand, within cesDat 2962.025 kb on - strand, within cesDat 2962.032 kb on - strand, within cesDat 2962.032 kb on - strand, within cesDat 2962.504 kb on - strand, within escCat 2962.771 kb on - strand, within escCat 2962.858 kb on - strand, within escCat 2963.127 kb on + strand, within escCat 2963.127 kb on + strand, within escC

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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2,960,806 - grlR OKFHMN_15315 0.90 +0.3
2,960,974 - grlA OKFHMN_15320 0.18 +0.3
2,961,253 - grlA OKFHMN_15320 0.86 -0.1
2,961,271 - -0.6
2,961,468 + -1.2
2,961,484 + +0.3
2,961,726 - -0.3
2,961,761 + cesD OKFHMN_15325 0.14 +0.6
2,961,823 + cesD OKFHMN_15325 0.28 -0.2
2,962,024 + cesD OKFHMN_15325 0.72 +0.9
2,962,025 - cesD OKFHMN_15325 0.72 -0.1
2,962,032 - cesD OKFHMN_15325 0.74 -1.2
2,962,032 - cesD OKFHMN_15325 0.74 -0.9
2,962,504 - escC OKFHMN_15330 0.22 +0.3
2,962,771 - escC OKFHMN_15330 0.39 +0.5
2,962,858 - escC OKFHMN_15330 0.45 -1.0
2,963,127 + escC OKFHMN_15330 0.63 -0.6
2,963,127 + escC OKFHMN_15330 0.63 -0.1

Or see this region's nucleotide sequence