Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt 23S_rrna and Ala_trna are separated by 183 nucleotides Ala_trna and Ile_trna are separated by 42 nucleotides Ile_trna and 16S_rrna are separated by 68 nucleotides 16S_rrna and hemG are separated by 380 nucleotides hemG and trkH are separated by 11 nucleotides
OKFHMN_14200: 23S_rrna - 23S ribosomal RNA, at 2,738,496 to 2,741,400
_rrna
OKFHMN_14210: Ala_trna - tRNA-Ala(tgc), at 2,741,584 to 2,741,659
_trna
OKFHMN_14215: Ile_trna - tRNA-Ile(gat), at 2,741,702 to 2,741,778
_trna
OKFHMN_14220: 16S_rrna - 16S ribosomal RNA, at 2,741,847 to 2,743,388
_rrna
OKFHMN_14225: hemG - menaquinone-dependent protoporphyrinogen IX dehydrogenase, at 2,743,769 to 2,744,314
hemG
OKFHMN_14230: trkH - Trk system potassium transporter TrkH, at 2,744,326 to 2,745,777
trkH
Position (kb)
2741
2742
2743
2744 Strain fitness (log2 ratio)
-2
-1
0
1 at 2741.843 kb on - strand at 2741.848 kb on - strand at 2743.771 kb on - strand at 2744.316 kb on + strand at 2744.316 kb on + strand at 2744.317 kb on - strand at 2744.317 kb on - strand at 2744.317 kb on - strand at 2744.319 kb on + strand at 2744.319 kb on + strand at 2744.319 kb on + strand at 2744.320 kb on - strand at 2744.320 kb on - strand at 2744.320 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 2,741,843 - +0.3 2,741,848 - +0.0 2,743,771 - -0.7 2,744,316 + +0.7 2,744,316 + -2.2 2,744,317 - -0.9 2,744,317 - +0.1 2,744,317 - -1.9 2,744,319 + -0.9 2,744,319 + -0.9 2,744,319 + -1.1 2,744,320 - +0.5 2,744,320 - -1.0 2,744,320 - +0.2
Or see this region's nucleotide sequence