Strain Fitness in Escherichia coli ECRC100 around OKFHMN_10875

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntkefC and kefF overlap by 8 nucleotideskefF and yaaU are separated by 107 nucleotidesyaaU and fixX are separated by 57 nucleotidesfixX and fixX overlap by 4 nucleotides OKFHMN_10865: kefC - glutathione-regulated potassium-efflux system protein KefC, at 2,049,131 to 2,050,993 kefC OKFHMN_10870: kefF - glutathione-regulated potassium-efflux system oxidoreductase KefF, at 2,050,986 to 2,051,516 kefF OKFHMN_10875: yaaU - Putative metabolite transport protein YaaU, at 2,051,624 to 2,052,955 yaaU OKFHMN_10880: fixX - ferredoxin-like protein FixX, at 2,053,013 to 2,053,300 fixX OKFHMN_10885: fixX - ferredoxin-like protein FixX, at 2,053,297 to 2,054,583 fixX Position (kb) 2051 2052 2053Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 2050.678 kb on + strand, within kefCat 2050.901 kb on + strandat 2050.981 kb on + strandat 2050.981 kb on + strandat 2050.981 kb on + strandat 2050.982 kb on - strandat 2051.027 kb on + strandat 2051.028 kb on - strandat 2051.254 kb on + strand, within kefFat 2051.280 kb on - strand, within kefFat 2051.314 kb on + strand, within kefFat 2051.315 kb on - strand, within kefFat 2051.565 kb on - strandat 2051.565 kb on - strandat 2051.567 kb on - strandat 2051.567 kb on - strandat 2051.567 kb on - strandat 2051.567 kb on - strandat 2051.580 kb on - strandat 2051.581 kb on + strandat 2051.654 kb on - strandat 2051.755 kb on + strandat 2051.767 kb on - strand, within yaaUat 2051.767 kb on - strand, within yaaUat 2051.770 kb on + strand, within yaaUat 2051.786 kb on + strand, within yaaUat 2051.823 kb on - strand, within yaaUat 2051.823 kb on - strand, within yaaUat 2051.830 kb on + strand, within yaaUat 2051.830 kb on + strand, within yaaUat 2051.831 kb on - strand, within yaaUat 2051.878 kb on + strand, within yaaUat 2051.879 kb on - strand, within yaaUat 2051.925 kb on + strand, within yaaUat 2052.079 kb on + strand, within yaaUat 2052.168 kb on + strand, within yaaUat 2052.169 kb on - strand, within yaaUat 2052.460 kb on - strand, within yaaUat 2052.586 kb on - strand, within yaaUat 2052.732 kb on + strand, within yaaUat 2052.733 kb on - strand, within yaaUat 2052.733 kb on - strand, within yaaUat 2052.812 kb on + strand, within yaaUat 2052.857 kb on + strandat 2052.859 kb on + strandat 2052.859 kb on + strandat 2052.859 kb on + strandat 2052.860 kb on - strandat 2052.860 kb on - strandat 2052.860 kb on - strandat 2052.860 kb on - strandat 2052.861 kb on + strandat 2052.861 kb on + strandat 2052.862 kb on - strandat 2052.862 kb on - strandat 2052.997 kb on - strandat 2053.000 kb on + strandat 2053.000 kb on + strandat 2053.001 kb on - strandat 2053.300 kb on + strandat 2053.496 kb on + strand, within fixXat 2053.500 kb on + strand, within fixXat 2053.500 kb on + strand, within fixXat 2053.501 kb on - strand, within fixXat 2053.501 kb on - strand, within fixXat 2053.501 kb on - strand, within fixXat 2053.501 kb on - strand, within fixXat 2053.506 kb on + strand, within fixXat 2053.507 kb on - strand, within fixXat 2053.542 kb on - strand, within fixXat 2053.636 kb on - strand, within fixXat 2053.663 kb on - strand, within fixXat 2053.663 kb on - strand, within fixXat 2053.745 kb on - strand, within fixXat 2053.903 kb on - strand, within fixXat 2053.952 kb on - strand, within fixX

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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2,050,678 + kefC OKFHMN_10865 0.83 -0.0
2,050,901 + -0.2
2,050,981 + -0.1
2,050,981 + +0.0
2,050,981 + -0.4
2,050,982 - -0.6
2,051,027 + -0.3
2,051,028 - +0.6
2,051,254 + kefF OKFHMN_10870 0.50 +0.0
2,051,280 - kefF OKFHMN_10870 0.55 -0.3
2,051,314 + kefF OKFHMN_10870 0.62 -0.5
2,051,315 - kefF OKFHMN_10870 0.62 +0.8
2,051,565 - -0.4
2,051,565 - +2.4
2,051,567 - +0.3
2,051,567 - -0.2
2,051,567 - +0.8
2,051,567 - -0.2
2,051,580 - +0.1
2,051,581 + +0.4
2,051,654 - +0.5
2,051,755 + -1.9
2,051,767 - yaaU OKFHMN_10875 0.11 -0.3
2,051,767 - yaaU OKFHMN_10875 0.11 -0.4
2,051,770 + yaaU OKFHMN_10875 0.11 +1.0
2,051,786 + yaaU OKFHMN_10875 0.12 -1.1
2,051,823 - yaaU OKFHMN_10875 0.15 +0.2
2,051,823 - yaaU OKFHMN_10875 0.15 -0.5
2,051,830 + yaaU OKFHMN_10875 0.15 -0.4
2,051,830 + yaaU OKFHMN_10875 0.15 -0.0
2,051,831 - yaaU OKFHMN_10875 0.16 +0.4
2,051,878 + yaaU OKFHMN_10875 0.19 +1.5
2,051,879 - yaaU OKFHMN_10875 0.19 -0.0
2,051,925 + yaaU OKFHMN_10875 0.23 +0.3
2,052,079 + yaaU OKFHMN_10875 0.34 -0.4
2,052,168 + yaaU OKFHMN_10875 0.41 +1.0
2,052,169 - yaaU OKFHMN_10875 0.41 +0.3
2,052,460 - yaaU OKFHMN_10875 0.63 -1.4
2,052,586 - yaaU OKFHMN_10875 0.72 -0.8
2,052,732 + yaaU OKFHMN_10875 0.83 -1.0
2,052,733 - yaaU OKFHMN_10875 0.83 +0.2
2,052,733 - yaaU OKFHMN_10875 0.83 -0.3
2,052,812 + yaaU OKFHMN_10875 0.89 +0.3
2,052,857 + -1.5
2,052,859 + -0.8
2,052,859 + +0.0
2,052,859 + +0.3
2,052,860 - +0.2
2,052,860 - +0.0
2,052,860 - -0.6
2,052,860 - -0.6
2,052,861 + +0.6
2,052,861 + +0.4
2,052,862 - -0.0
2,052,862 - -3.7
2,052,997 - -0.7
2,053,000 + -4.0
2,053,000 + -1.1
2,053,001 - -0.0
2,053,300 + -0.7
2,053,496 + fixX OKFHMN_10885 0.15 +0.2
2,053,500 + fixX OKFHMN_10885 0.16 -0.8
2,053,500 + fixX OKFHMN_10885 0.16 +0.3
2,053,501 - fixX OKFHMN_10885 0.16 +0.9
2,053,501 - fixX OKFHMN_10885 0.16 +0.6
2,053,501 - fixX OKFHMN_10885 0.16 +0.3
2,053,501 - fixX OKFHMN_10885 0.16 -1.1
2,053,506 + fixX OKFHMN_10885 0.16 -0.5
2,053,507 - fixX OKFHMN_10885 0.16 -0.3
2,053,542 - fixX OKFHMN_10885 0.19 +1.2
2,053,636 - fixX OKFHMN_10885 0.26 -1.1
2,053,663 - fixX OKFHMN_10885 0.28 +0.4
2,053,663 - fixX OKFHMN_10885 0.28 +2.3
2,053,745 - fixX OKFHMN_10885 0.35 +0.9
2,053,903 - fixX OKFHMN_10885 0.47 +0.1
2,053,952 - fixX OKFHMN_10885 0.51 +1.9

Or see this region's nucleotide sequence