Strain Fitness in Escherichia coli ECRC100 around OKFHMN_05185

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyccA and tusE are separated by 90 nucleotidestusE and yccX overlap by 4 nucleotidesyccX and rlmI are separated by 109 nucleotidesrlmI and hspQ are separated by 57 nucleotideshspQ and yccU are separated by 44 nucleotides OKFHMN_05170: yccA - FtsH protease modulator YccA, at 855,279 to 855,938 yccA OKFHMN_05175: tusE - sulfurtransferase TusE, at 856,029 to 856,358 tusE OKFHMN_05180: yccX - acylphosphatase, at 856,355 to 856,633 yccX OKFHMN_05185: rlmI - 23S rRNA (cytosine(1962)-C(5))-methyltransferase RlmI, at 856,743 to 857,918 rlmI OKFHMN_05190: hspQ - heat shock protein HspQ, at 857,976 to 858,293 hspQ OKFHMN_05195: yccU - Uncharacterized protein YccU, at 858,338 to 858,751 yccU Position (kb) 856 857 858Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 855.873 kb on + strandat 856.013 kb on + strandat 856.024 kb on + strandat 856.356 kb on + strandat 856.357 kb on - strandat 856.460 kb on + strand, within yccXat 856.470 kb on - strand, within yccXat 856.561 kb on - strand, within yccXat 856.727 kb on + strandat 856.728 kb on - strandat 856.735 kb on + strandat 856.735 kb on + strandat 856.824 kb on - strandat 857.032 kb on - strand, within rlmIat 857.072 kb on + strand, within rlmIat 857.072 kb on + strand, within rlmIat 857.337 kb on + strand, within rlmIat 857.582 kb on + strand, within rlmIat 857.770 kb on + strand, within rlmIat 857.867 kb on + strandat 857.886 kb on + strandat 857.942 kb on + strandat 857.944 kb on + strandat 858.170 kb on + strand, within hspQat 858.170 kb on + strand, within hspQat 858.174 kb on + strand, within hspQat 858.193 kb on + strand, within hspQat 858.340 kb on - strandat 858.482 kb on - strand, within yccUat 858.648 kb on + strand, within yccUat 858.649 kb on - strand, within yccUat 858.723 kb on - strandat 858.843 kb on + strandat 858.844 kb on - strandat 858.849 kb on + strandat 858.849 kb on + strandat 858.849 kb on + strandat 858.850 kb on - strandat 858.852 kb on - strandat 858.860 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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855,873 + +0.6
856,013 + +0.7
856,024 + -1.6
856,356 + +0.3
856,357 - -0.3
856,460 + yccX OKFHMN_05180 0.38 -0.6
856,470 - yccX OKFHMN_05180 0.41 +0.8
856,561 - yccX OKFHMN_05180 0.74 -0.2
856,727 + -3.3
856,728 - +0.2
856,735 + +0.2
856,735 + -0.8
856,824 - +0.0
857,032 - rlmI OKFHMN_05185 0.25 -0.6
857,072 + rlmI OKFHMN_05185 0.28 -0.2
857,072 + rlmI OKFHMN_05185 0.28 +1.5
857,337 + rlmI OKFHMN_05185 0.51 +0.5
857,582 + rlmI OKFHMN_05185 0.71 -0.9
857,770 + rlmI OKFHMN_05185 0.87 +0.4
857,867 + +0.9
857,886 + +0.3
857,942 + +0.7
857,944 + -0.1
858,170 + hspQ OKFHMN_05190 0.61 -0.1
858,170 + hspQ OKFHMN_05190 0.61 -3.3
858,174 + hspQ OKFHMN_05190 0.62 -0.0
858,193 + hspQ OKFHMN_05190 0.68 -0.4
858,340 - -1.2
858,482 - yccU OKFHMN_05195 0.35 -0.2
858,648 + yccU OKFHMN_05195 0.75 +2.0
858,649 - yccU OKFHMN_05195 0.75 +0.3
858,723 - -0.6
858,843 + -1.3
858,844 - +1.1
858,849 + -2.8
858,849 + -0.0
858,849 + +0.9
858,850 - +0.6
858,852 - -0.7
858,860 - -0.1

Or see this region's nucleotide sequence