Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt putP and OKFHMN_04600 are separated by 158 nucleotides OKFHMN_04600 and putA are separated by 54 nucleotides putA and rutR are separated by 39 nucleotides rutR and rutA are separated by 287 nucleotides
OKFHMN_04595: putP - sodium/proline symporter PutP, at 757,101 to 758,609
putP
OKFHMN_04600: OKFHMN_04600 - hypothetical protein, at 758,768 to 758,977
_04600
OKFHMN_04605: putA - trifunctional transcriptional regulator/proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase, at 759,032 to 762,994
putA
OKFHMN_04610: rutR - HTH-type transcriptional regulator RutR, at 763,034 to 763,672
rutR
OKFHMN_04615: rutA - pyrimidine utilization protein A, at 763,960 to 765,051
rutA
Position (kb) 759
760
761
762
763 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 758.090 kb on - strand, within putP at 758.148 kb on - strand, within putP at 758.273 kb on - strand, within putP at 758.294 kb on + strand, within putP at 758.505 kb on - strand at 758.505 kb on - strand at 758.507 kb on - strand at 758.611 kb on + strand at 758.689 kb on - strand at 758.695 kb on + strand at 758.904 kb on - strand, within OKFHMN_04600 at 758.981 kb on + strand at 759.083 kb on - strand at 759.085 kb on + strand at 759.126 kb on + strand at 759.355 kb on + strand at 759.356 kb on - strand at 759.700 kb on + strand, within putA at 759.881 kb on + strand, within putA at 759.929 kb on + strand, within putA at 759.993 kb on - strand, within putA at 759.993 kb on - strand, within putA at 760.412 kb on + strand, within putA at 760.412 kb on + strand, within putA at 760.414 kb on + strand, within putA at 760.468 kb on - strand, within putA at 760.717 kb on + strand, within putA at 760.718 kb on - strand, within putA at 761.054 kb on + strand, within putA at 761.054 kb on + strand, within putA at 761.058 kb on + strand, within putA at 761.058 kb on + strand, within putA at 761.285 kb on + strand, within putA at 761.349 kb on + strand, within putA at 761.349 kb on + strand, within putA at 761.353 kb on + strand, within putA at 761.427 kb on + strand, within putA at 761.740 kb on - strand, within putA at 762.249 kb on - strand, within putA at 762.507 kb on + strand, within putA at 762.507 kb on + strand, within putA at 762.508 kb on - strand, within putA at 762.511 kb on + strand, within putA at 762.519 kb on - strand, within putA at 762.609 kb on + strand at 762.714 kb on + strand at 762.923 kb on + strand at 762.923 kb on + strand at 762.923 kb on + strand at 762.926 kb on - strand at 763.014 kb on + strand at 763.015 kb on - strand at 763.061 kb on - strand at 763.476 kb on + strand, within rutR at 763.476 kb on + strand, within rutR at 763.477 kb on - strand, within rutR at 763.477 kb on - strand, within rutR at 763.766 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 758,090 - putP OKFHMN_04595 0.66 -1.8 758,148 - putP OKFHMN_04595 0.69 -0.1 758,273 - putP OKFHMN_04595 0.78 +0.2 758,294 + putP OKFHMN_04595 0.79 -0.7 758,505 - +0.8 758,505 - -0.7 758,507 - +0.7 758,611 + -1.3 758,689 - +0.2 758,695 + -0.4 758,904 - OKFHMN_04600 0.65 -1.2 758,981 + +0.8 759,083 - -0.2 759,085 + +0.5 759,126 + -0.3 759,355 + -0.1 759,356 - -2.0 759,700 + putA OKFHMN_04605 0.17 -0.0 759,881 + putA OKFHMN_04605 0.21 -0.3 759,929 + putA OKFHMN_04605 0.23 -0.3 759,993 - putA OKFHMN_04605 0.24 -3.3 759,993 - putA OKFHMN_04605 0.24 +0.3 760,412 + putA OKFHMN_04605 0.35 -1.1 760,412 + putA OKFHMN_04605 0.35 -0.5 760,414 + putA OKFHMN_04605 0.35 +0.9 760,468 - putA OKFHMN_04605 0.36 +0.6 760,717 + putA OKFHMN_04605 0.43 -0.0 760,718 - putA OKFHMN_04605 0.43 -0.6 761,054 + putA OKFHMN_04605 0.51 -0.4 761,054 + putA OKFHMN_04605 0.51 -0.5 761,058 + putA OKFHMN_04605 0.51 +1.9 761,058 + putA OKFHMN_04605 0.51 -0.3 761,285 + putA OKFHMN_04605 0.57 -0.2 761,349 + putA OKFHMN_04605 0.58 -0.6 761,349 + putA OKFHMN_04605 0.58 -3.0 761,353 + putA OKFHMN_04605 0.59 +0.3 761,427 + putA OKFHMN_04605 0.60 +1.5 761,740 - putA OKFHMN_04605 0.68 -0.2 762,249 - putA OKFHMN_04605 0.81 -0.4 762,507 + putA OKFHMN_04605 0.88 +0.7 762,507 + putA OKFHMN_04605 0.88 +0.4 762,508 - putA OKFHMN_04605 0.88 -2.3 762,511 + putA OKFHMN_04605 0.88 -0.7 762,519 - putA OKFHMN_04605 0.88 -1.0 762,609 + +1.7 762,714 + -0.1 762,923 + -0.5 762,923 + -0.7 762,923 + -2.0 762,926 - -0.7 763,014 + +0.3 763,015 - -1.2 763,061 - +1.3 763,476 + rutR OKFHMN_04610 0.69 +1.5 763,476 + rutR OKFHMN_04610 0.69 +2.1 763,477 - rutR OKFHMN_04610 0.69 +0.7 763,477 - rutR OKFHMN_04610 0.69 -0.0 763,766 - -0.8
Or see this region's nucleotide sequence