Strain Fitness in Escherichia coli ECRC100 around OKFHMN_02150

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrecT and OKFHMN_02135 are separated by 243 nucleotidesOKFHMN_02135 and xisR are separated by 99 nucleotidesxisR and fimB are separated by 1 nucleotidesfimB and ttcA are separated by 51 nucleotides OKFHMN_02130: recT - recombination protein RecT, at 320,888 to 321,697 recT OKFHMN_02135: OKFHMN_02135 - DUF1187 domain-containing protein, at 321,941 to 322,129 _02135 OKFHMN_02145: xisR - excisionase XisR, at 322,229 to 322,444 xisR OKFHMN_02150: fimB - site-specific integrase, at 322,446 to 323,681 fimB OKFHMN_02155: ttcA - tRNA 2-thiocytidine(32) synthetase TtcA, at 323,733 to 324,668 ttcA Position (kb) 322 323 324Strain fitness (log2 ratio) -3 -2 -1 0 1at 321.946 kb on + strandat 323.124 kb on + strand, within fimBat 323.266 kb on + strand, within fimBat 323.476 kb on + strand, within fimBat 323.680 kb on - strandat 323.769 kb on + strandat 324.191 kb on + strand, within ttcAat 324.589 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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321,946 + -0.6
323,124 + fimB OKFHMN_02150 0.55 -0.6
323,266 + fimB OKFHMN_02150 0.66 +1.7
323,476 + fimB OKFHMN_02150 0.83 +0.7
323,680 - -2.9
323,769 + -1.5
324,191 + ttcA OKFHMN_02155 0.49 +0.6
324,589 + -1.0

Or see this region's nucleotide sequence