Strain Fitness in Escherichia coli ECRC100 around OKFHMN_00620

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntespK and potB are separated by 363 nucleotidespotB and potA overlap by 17 nucleotides OKFHMN_00615: espK - type III secretion system effector EspK, at 28,852 to 30,213 espK OKFHMN_00620: potB - spermidine/putrescine ABC transporter permease PotB, at 30,577 to 31,440 potB OKFHMN_00625: potA - spermidine/putrescine ABC transporter ATP-binding protein PotA, at 31,424 to 32,560 potA Position (kb) 30 31 32Strain fitness (log2 ratio) -1 0 1at 29.640 kb on + strand, within espKat 29.781 kb on + strand, within espKat 30.716 kb on - strand, within potBat 30.774 kb on - strand, within potBat 30.866 kb on - strand, within potBat 31.284 kb on - strand, within potBat 31.284 kb on - strand, within potBat 31.399 kb on - strandat 31.964 kb on - strand, within potAat 31.976 kb on - strand, within potA

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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29,640 + espK OKFHMN_00615 0.58 +1.0
29,781 + espK OKFHMN_00615 0.68 +0.6
30,716 - potB OKFHMN_00620 0.16 -0.6
30,774 - potB OKFHMN_00620 0.23 -0.9
30,866 - potB OKFHMN_00620 0.33 -0.1
31,284 - potB OKFHMN_00620 0.82 -1.3
31,284 - potB OKFHMN_00620 0.82 -0.2
31,399 - +0.2
31,964 - potA OKFHMN_00625 0.47 -1.7
31,976 - potA OKFHMN_00625 0.49 -0.0

Or see this region's nucleotide sequence