Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt folX and yfcG are separated by 56 nucleotides yfcG and yfcF are separated by 135 nucleotides yfcF and yfcE are separated by 55 nucleotides yfcE and yfcD are separated by 57 nucleotides
HEPCGN_21965: folX - dihydroneopterin triphosphate 2'-epimerase, at 3,148,770 to 3,149,132
folX
HEPCGN_21970: yfcG - GSH-dependent disulfide bond oxidoreductase, at 3,149,189 to 3,149,836
yfcG
HEPCGN_21975: yfcF - glutathione transferase, at 3,149,972 to 3,150,616
yfcF
HEPCGN_21980: yfcE - phosphodiesterase, at 3,150,672 to 3,151,223
yfcE
HEPCGN_21985: yfcD - NUDIX hydrolase YfcD, at 3,151,281 to 3,151,823
yfcD
Position (kb)
3149
3150
3151 Strain fitness (log2 ratio)
-1
0
1
2 at 3149.084 kb on + strand, within folX at 3149.103 kb on + strand at 3149.111 kb on + strand at 3149.111 kb on + strand at 3149.157 kb on - strand at 3149.157 kb on - strand at 3149.166 kb on + strand at 3149.169 kb on + strand at 3149.408 kb on + strand, within yfcG at 3149.409 kb on - strand, within yfcG at 3149.482 kb on + strand, within yfcG at 3149.534 kb on + strand, within yfcG at 3149.543 kb on + strand, within yfcG at 3149.544 kb on - strand, within yfcG at 3149.823 kb on + strand at 3149.854 kb on - strand at 3149.875 kb on - strand at 3149.877 kb on - strand at 3149.934 kb on + strand at 3149.970 kb on + strand at 3149.970 kb on + strand at 3149.970 kb on + strand at 3149.970 kb on + strand at 3149.971 kb on - strand at 3149.977 kb on + strand at 3149.978 kb on - strand at 3150.023 kb on + strand at 3150.023 kb on + strand at 3150.023 kb on + strand at 3150.073 kb on + strand, within yfcF at 3150.156 kb on + strand, within yfcF at 3150.241 kb on + strand, within yfcF at 3150.242 kb on - strand, within yfcF at 3150.476 kb on - strand, within yfcF at 3150.510 kb on + strand, within yfcF at 3150.511 kb on - strand, within yfcF at 3150.641 kb on + strand at 3150.641 kb on + strand at 3150.809 kb on - strand, within yfcE at 3150.863 kb on + strand, within yfcE at 3150.863 kb on + strand, within yfcE at 3150.864 kb on - strand, within yfcE at 3150.864 kb on - strand, within yfcE at 3150.864 kb on - strand, within yfcE at 3150.864 kb on - strand, within yfcE at 3150.864 kb on - strand, within yfcE at 3150.949 kb on + strand, within yfcE at 3150.949 kb on + strand, within yfcE at 3150.949 kb on + strand, within yfcE at 3150.950 kb on - strand, within yfcE at 3150.950 kb on - strand, within yfcE at 3150.950 kb on - strand, within yfcE at 3151.112 kb on + strand, within yfcE at 3151.119 kb on - strand, within yfcE at 3151.119 kb on - strand, within yfcE at 3151.162 kb on + strand, within yfcE at 3151.162 kb on + strand, within yfcE at 3151.162 kb on + strand, within yfcE at 3151.162 kb on + strand, within yfcE at 3151.162 kb on + strand, within yfcE at 3151.163 kb on - strand, within yfcE at 3151.172 kb on - strand at 3151.172 kb on - strand at 3151.401 kb on + strand, within yfcD at 3151.401 kb on + strand, within yfcD at 3151.401 kb on + strand, within yfcD at 3151.401 kb on + strand, within yfcD at 3151.401 kb on + strand, within yfcD at 3151.402 kb on - strand, within yfcD at 3151.481 kb on - strand, within yfcD
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 3,149,084 + folX HEPCGN_21965 0.87 +0.6 3,149,103 + -1.4 3,149,111 + +0.1 3,149,111 + -0.6 3,149,157 - +1.1 3,149,157 - +0.2 3,149,166 + +0.3 3,149,169 + +0.0 3,149,408 + yfcG HEPCGN_21970 0.34 +0.6 3,149,409 - yfcG HEPCGN_21970 0.34 +0.2 3,149,482 + yfcG HEPCGN_21970 0.45 -0.0 3,149,534 + yfcG HEPCGN_21970 0.53 -0.1 3,149,543 + yfcG HEPCGN_21970 0.55 +0.1 3,149,544 - yfcG HEPCGN_21970 0.55 +0.9 3,149,823 + +0.4 3,149,854 - -0.3 3,149,875 - +0.5 3,149,877 - -0.3 3,149,934 + -0.3 3,149,970 + +0.1 3,149,970 + +0.6 3,149,970 + -0.6 3,149,970 + +0.0 3,149,971 - -0.1 3,149,977 + -0.2 3,149,978 - +0.4 3,150,023 + +0.0 3,150,023 + +0.1 3,150,023 + -0.9 3,150,073 + yfcF HEPCGN_21975 0.16 +0.1 3,150,156 + yfcF HEPCGN_21975 0.29 -0.0 3,150,241 + yfcF HEPCGN_21975 0.42 -0.4 3,150,242 - yfcF HEPCGN_21975 0.42 -1.0 3,150,476 - yfcF HEPCGN_21975 0.78 +0.3 3,150,510 + yfcF HEPCGN_21975 0.83 -0.5 3,150,511 - yfcF HEPCGN_21975 0.84 -0.7 3,150,641 + -0.4 3,150,641 + +0.4 3,150,809 - yfcE HEPCGN_21980 0.25 +0.6 3,150,863 + yfcE HEPCGN_21980 0.35 -0.2 3,150,863 + yfcE HEPCGN_21980 0.35 -1.0 3,150,864 - yfcE HEPCGN_21980 0.35 -0.6 3,150,864 - yfcE HEPCGN_21980 0.35 -0.5 3,150,864 - yfcE HEPCGN_21980 0.35 -0.1 3,150,864 - yfcE HEPCGN_21980 0.35 -0.2 3,150,864 - yfcE HEPCGN_21980 0.35 +0.2 3,150,949 + yfcE HEPCGN_21980 0.50 +2.3 3,150,949 + yfcE HEPCGN_21980 0.50 +1.0 3,150,949 + yfcE HEPCGN_21980 0.50 -0.2 3,150,950 - yfcE HEPCGN_21980 0.50 +0.0 3,150,950 - yfcE HEPCGN_21980 0.50 -0.6 3,150,950 - yfcE HEPCGN_21980 0.50 -1.6 3,151,112 + yfcE HEPCGN_21980 0.80 -0.1 3,151,119 - yfcE HEPCGN_21980 0.81 +0.0 3,151,119 - yfcE HEPCGN_21980 0.81 -0.8 3,151,162 + yfcE HEPCGN_21980 0.89 +0.3 3,151,162 + yfcE HEPCGN_21980 0.89 +0.1 3,151,162 + yfcE HEPCGN_21980 0.89 +0.3 3,151,162 + yfcE HEPCGN_21980 0.89 -0.4 3,151,162 + yfcE HEPCGN_21980 0.89 +0.2 3,151,163 - yfcE HEPCGN_21980 0.89 +2.2 3,151,172 - -0.1 3,151,172 - -1.4 3,151,401 + yfcD HEPCGN_21985 0.22 +0.1 3,151,401 + yfcD HEPCGN_21985 0.22 -1.2 3,151,401 + yfcD HEPCGN_21985 0.22 -0.2 3,151,401 + yfcD HEPCGN_21985 0.22 -0.7 3,151,401 + yfcD HEPCGN_21985 0.22 -0.1 3,151,402 - yfcD HEPCGN_21985 0.22 +0.5 3,151,481 - yfcD HEPCGN_21985 0.37 -0.1
Or see this region's nucleotide sequence