Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hyfF and hyfE are separated by 4 nucleotides hyfE and hyfD are separated by 11 nucleotides hyfD and hyfC are separated by 16 nucleotides hyfC and hyfB are separated by 10 nucleotides
HEPCGN_21045: hyfF - hydrogenase 4 subunit F, at 2,960,366 to 2,961,946
hyfF
HEPCGN_21050: hyfE - hydrogenase 4 membrane subunit, at 2,961,951 to 2,962,601
hyfE
HEPCGN_21055: hyfD - hydrogenase 4 subunit HyfD, at 2,962,613 to 2,964,052
hyfD
HEPCGN_21060: hyfC - hydrogenase 4 subunit HyfC, at 2,964,069 to 2,965,016
hyfC
HEPCGN_21065: hyfB - hydrogenase 4 subunit B, at 2,965,027 to 2,967,045
hyfB
Position (kb)
2962
2963
2964
2965 Strain fitness (log2 ratio)
-1
0
1 at 2961.657 kb on + strand, within hyfF at 2961.658 kb on - strand, within hyfF at 2961.734 kb on - strand, within hyfF at 2961.758 kb on + strand, within hyfF at 2961.759 kb on - strand, within hyfF at 2961.824 kb on + strand at 2961.829 kb on + strand at 2961.829 kb on + strand at 2961.830 kb on - strand at 2961.830 kb on - strand at 2961.833 kb on + strand at 2961.833 kb on + strand at 2961.833 kb on + strand at 2961.833 kb on + strand at 2961.834 kb on - strand at 2961.837 kb on - strand at 2961.940 kb on - strand at 2962.207 kb on - strand, within hyfE at 2962.207 kb on - strand, within hyfE at 2962.249 kb on - strand, within hyfE at 2962.291 kb on - strand, within hyfE at 2962.314 kb on - strand, within hyfE at 2962.355 kb on + strand, within hyfE at 2962.356 kb on - strand, within hyfE at 2962.369 kb on + strand, within hyfE at 2962.370 kb on - strand, within hyfE at 2962.370 kb on - strand, within hyfE at 2962.522 kb on + strand, within hyfE at 2962.581 kb on + strand at 2962.589 kb on + strand at 2962.589 kb on + strand at 2962.590 kb on - strand at 2962.590 kb on - strand at 2962.668 kb on - strand at 2962.998 kb on + strand, within hyfD at 2963.028 kb on - strand, within hyfD at 2963.168 kb on - strand, within hyfD at 2963.184 kb on + strand, within hyfD at 2963.185 kb on - strand, within hyfD at 2963.200 kb on - strand, within hyfD at 2963.221 kb on + strand, within hyfD at 2963.221 kb on + strand, within hyfD at 2963.326 kb on - strand, within hyfD at 2963.468 kb on - strand, within hyfD at 2963.514 kb on + strand, within hyfD at 2963.514 kb on + strand, within hyfD at 2963.514 kb on + strand, within hyfD at 2963.534 kb on - strand, within hyfD at 2963.542 kb on + strand, within hyfD at 2963.542 kb on + strand, within hyfD at 2963.542 kb on + strand, within hyfD at 2963.546 kb on + strand, within hyfD at 2963.586 kb on + strand, within hyfD at 2963.587 kb on - strand, within hyfD at 2963.687 kb on - strand, within hyfD at 2964.070 kb on + strand at 2964.385 kb on - strand, within hyfC at 2964.469 kb on - strand, within hyfC at 2964.688 kb on + strand, within hyfC at 2964.688 kb on + strand, within hyfC at 2964.692 kb on + strand, within hyfC at 2964.737 kb on + strand, within hyfC at 2964.738 kb on - strand, within hyfC at 2964.738 kb on - strand, within hyfC at 2964.738 kb on - strand, within hyfC at 2964.738 kb on - strand, within hyfC at 2964.741 kb on + strand, within hyfC at 2964.742 kb on - strand, within hyfC at 2964.912 kb on + strand, within hyfC at 2964.913 kb on - strand, within hyfC at 2965.045 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 2,961,657 + hyfF HEPCGN_21045 0.82 -0.5 2,961,658 - hyfF HEPCGN_21045 0.82 +0.7 2,961,734 - hyfF HEPCGN_21045 0.87 +0.6 2,961,758 + hyfF HEPCGN_21045 0.88 +1.5 2,961,759 - hyfF HEPCGN_21045 0.88 -1.0 2,961,824 + -0.3 2,961,829 + +0.2 2,961,829 + -0.7 2,961,830 - -0.1 2,961,830 - +0.1 2,961,833 + -0.4 2,961,833 + -0.1 2,961,833 + -0.1 2,961,833 + +0.4 2,961,834 - +0.3 2,961,837 - +0.5 2,961,940 - +0.2 2,962,207 - hyfE HEPCGN_21050 0.39 -0.2 2,962,207 - hyfE HEPCGN_21050 0.39 +0.4 2,962,249 - hyfE HEPCGN_21050 0.46 +0.4 2,962,291 - hyfE HEPCGN_21050 0.52 -0.1 2,962,314 - hyfE HEPCGN_21050 0.56 -0.2 2,962,355 + hyfE HEPCGN_21050 0.62 -0.1 2,962,356 - hyfE HEPCGN_21050 0.62 -0.5 2,962,369 + hyfE HEPCGN_21050 0.64 +0.4 2,962,370 - hyfE HEPCGN_21050 0.64 -0.6 2,962,370 - hyfE HEPCGN_21050 0.64 -0.1 2,962,522 + hyfE HEPCGN_21050 0.88 +0.0 2,962,581 + +0.7 2,962,589 + +0.2 2,962,589 + -0.0 2,962,590 - +0.2 2,962,590 - +0.1 2,962,668 - +0.1 2,962,998 + hyfD HEPCGN_21055 0.27 +0.3 2,963,028 - hyfD HEPCGN_21055 0.29 -0.2 2,963,168 - hyfD HEPCGN_21055 0.39 -1.6 2,963,184 + hyfD HEPCGN_21055 0.40 -0.2 2,963,185 - hyfD HEPCGN_21055 0.40 -0.0 2,963,200 - hyfD HEPCGN_21055 0.41 -0.2 2,963,221 + hyfD HEPCGN_21055 0.42 +0.6 2,963,221 + hyfD HEPCGN_21055 0.42 +1.6 2,963,326 - hyfD HEPCGN_21055 0.50 -0.2 2,963,468 - hyfD HEPCGN_21055 0.59 +0.2 2,963,514 + hyfD HEPCGN_21055 0.63 +0.7 2,963,514 + hyfD HEPCGN_21055 0.63 +0.5 2,963,514 + hyfD HEPCGN_21055 0.63 +0.3 2,963,534 - hyfD HEPCGN_21055 0.64 -0.5 2,963,542 + hyfD HEPCGN_21055 0.65 +0.3 2,963,542 + hyfD HEPCGN_21055 0.65 +0.0 2,963,542 + hyfD HEPCGN_21055 0.65 +0.5 2,963,546 + hyfD HEPCGN_21055 0.65 +1.1 2,963,586 + hyfD HEPCGN_21055 0.68 -0.3 2,963,587 - hyfD HEPCGN_21055 0.68 +1.0 2,963,687 - hyfD HEPCGN_21055 0.75 -0.1 2,964,070 + +0.4 2,964,385 - hyfC HEPCGN_21060 0.33 -0.7 2,964,469 - hyfC HEPCGN_21060 0.42 +0.9 2,964,688 + hyfC HEPCGN_21060 0.65 +0.8 2,964,688 + hyfC HEPCGN_21060 0.65 -0.1 2,964,692 + hyfC HEPCGN_21060 0.66 +0.9 2,964,737 + hyfC HEPCGN_21060 0.70 -1.0 2,964,738 - hyfC HEPCGN_21060 0.71 -0.3 2,964,738 - hyfC HEPCGN_21060 0.71 +0.8 2,964,738 - hyfC HEPCGN_21060 0.71 -0.3 2,964,738 - hyfC HEPCGN_21060 0.71 +0.0 2,964,741 + hyfC HEPCGN_21060 0.71 +0.3 2,964,742 - hyfC HEPCGN_21060 0.71 +0.4 2,964,912 + hyfC HEPCGN_21060 0.89 +0.7 2,964,913 - hyfC HEPCGN_21060 0.89 -0.8 2,965,045 + +0.7
Or see this region's nucleotide sequence