Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt kpsM and kpsT overlap by 4 nucleotides kpsT and neuD are separated by 49 nucleotides neuD and neuB overlap by 4 nucleotides
HEPCGN_18100: kpsM - Polysialic acid transport protein, at 2,369,451 to 2,370,227
kpsM
HEPCGN_18105: kpsT - Polysialic acid transport ATP-binding protein, at 2,370,224 to 2,370,883
kpsT
HEPCGN_18110: neuD - NeuD protein involved in sialic acid synthesis, at 2,370,933 to 2,371,556
neuD
HEPCGN_18115: neuB - N-acylneuraminate-9-phosphate synthase, at 2,371,553 to 2,372,593
neuB
Position (kb)
2370
2371 Strain fitness (log2 ratio)
-2
-1
0
1 at 2369.390 kb on - strand at 2369.421 kb on + strand at 2369.621 kb on + strand, within kpsM at 2369.684 kb on + strand, within kpsM at 2369.685 kb on - strand, within kpsM at 2369.702 kb on + strand, within kpsM at 2369.711 kb on + strand, within kpsM at 2369.762 kb on + strand, within kpsM at 2369.811 kb on + strand, within kpsM at 2369.900 kb on - strand, within kpsM at 2369.933 kb on + strand, within kpsM at 2369.933 kb on + strand, within kpsM at 2370.046 kb on - strand, within kpsM at 2370.054 kb on + strand, within kpsM at 2370.058 kb on + strand, within kpsM at 2370.059 kb on - strand, within kpsM at 2370.059 kb on - strand, within kpsM at 2370.074 kb on - strand, within kpsM at 2370.074 kb on - strand, within kpsM at 2370.107 kb on + strand, within kpsM at 2370.144 kb on + strand, within kpsM at 2370.144 kb on + strand, within kpsM at 2370.320 kb on - strand, within kpsT at 2370.385 kb on - strand, within kpsT at 2370.385 kb on - strand, within kpsT at 2370.422 kb on - strand, within kpsT at 2371.265 kb on + strand, within neuD at 2371.367 kb on + strand, within neuD at 2371.425 kb on - strand, within neuD at 2371.506 kb on - strand at 2371.563 kb on + strand at 2371.565 kb on + strand at 2371.784 kb on + strand, within neuB
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 2,369,390 - +0.2 2,369,421 + +1.2 2,369,621 + kpsM HEPCGN_18100 0.22 +0.2 2,369,684 + kpsM HEPCGN_18100 0.30 -0.8 2,369,685 - kpsM HEPCGN_18100 0.30 -2.1 2,369,702 + kpsM HEPCGN_18100 0.32 -1.8 2,369,711 + kpsM HEPCGN_18100 0.33 +0.4 2,369,762 + kpsM HEPCGN_18100 0.40 -0.2 2,369,811 + kpsM HEPCGN_18100 0.46 +0.6 2,369,900 - kpsM HEPCGN_18100 0.58 +0.1 2,369,933 + kpsM HEPCGN_18100 0.62 -2.0 2,369,933 + kpsM HEPCGN_18100 0.62 -1.1 2,370,046 - kpsM HEPCGN_18100 0.77 -0.1 2,370,054 + kpsM HEPCGN_18100 0.78 +0.3 2,370,058 + kpsM HEPCGN_18100 0.78 -2.7 2,370,059 - kpsM HEPCGN_18100 0.78 +0.9 2,370,059 - kpsM HEPCGN_18100 0.78 -0.2 2,370,074 - kpsM HEPCGN_18100 0.80 +1.0 2,370,074 - kpsM HEPCGN_18100 0.80 +0.3 2,370,107 + kpsM HEPCGN_18100 0.84 -0.2 2,370,144 + kpsM HEPCGN_18100 0.89 -0.1 2,370,144 + kpsM HEPCGN_18100 0.89 +0.2 2,370,320 - kpsT HEPCGN_18105 0.15 +0.8 2,370,385 - kpsT HEPCGN_18105 0.24 -0.5 2,370,385 - kpsT HEPCGN_18105 0.24 -0.3 2,370,422 - kpsT HEPCGN_18105 0.30 +0.6 2,371,265 + neuD HEPCGN_18110 0.53 +0.7 2,371,367 + neuD HEPCGN_18110 0.70 +0.6 2,371,425 - neuD HEPCGN_18110 0.79 +1.1 2,371,506 - +0.7 2,371,563 + +0.6 2,371,565 + +0.2 2,371,784 + neuB HEPCGN_18115 0.22 +0.4
Or see this region's nucleotide sequence