Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt leuC1 and leuD are separated by 10 nucleotides leuD and HEPCGN_08720 are separated by 107 nucleotides HEPCGN_08720 and sgrR are separated by 88 nucleotides
HEPCGN_08705: leuC1 - 3-isopropylmalate dehydratase large subunit 1, at 481,530 to 482,930
leuC1
HEPCGN_08710: leuD - 3-isopropylmalate dehydratase small subunit, at 482,941 to 483,546
leuD
HEPCGN_08720: HEPCGN_08720 - glucose uptake inhibitor SgrT, at 483,654 to 483,785
_08720
HEPCGN_08725: sgrR - DNA-binding transcriptional regulator SgrR, at 483,874 to 485,532
sgrR
Position (kb) 483
484 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 482.674 kb on + strand, within leuC1 at 482.924 kb on + strand at 482.924 kb on + strand at 482.964 kb on + strand at 482.965 kb on - strand at 482.965 kb on - strand at 483.142 kb on + strand, within leuD at 483.143 kb on - strand, within leuD at 483.235 kb on - strand, within leuD at 483.514 kb on + strand at 483.537 kb on + strand at 483.544 kb on + strand at 483.552 kb on - strand at 483.596 kb on + strand at 483.597 kb on - strand at 483.627 kb on + strand at 483.627 kb on + strand at 483.701 kb on + strand, within HEPCGN_08720 at 483.702 kb on - strand, within HEPCGN_08720 at 483.705 kb on + strand, within HEPCGN_08720 at 483.764 kb on - strand, within HEPCGN_08720 at 483.824 kb on + strand at 483.825 kb on - strand at 483.825 kb on - strand at 483.825 kb on - strand at 484.252 kb on + strand, within sgrR at 484.289 kb on + strand, within sgrR at 484.500 kb on + strand, within sgrR at 484.758 kb on - strand, within sgrR at 484.771 kb on - strand, within sgrR
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 482,674 + leuC1 HEPCGN_08705 0.82 -4.2 482,924 + -0.6 482,924 + +0.4 482,964 + -3.0 482,965 - -4.1 482,965 - -4.0 483,142 + leuD HEPCGN_08710 0.33 -4.5 483,143 - leuD HEPCGN_08710 0.33 -4.5 483,235 - leuD HEPCGN_08710 0.49 -4.3 483,514 + -5.2 483,537 + +1.7 483,544 + +0.6 483,552 - -0.1 483,596 + +0.6 483,597 - -0.0 483,627 + -0.2 483,627 + +0.7 483,701 + HEPCGN_08720 0.36 -1.7 483,702 - HEPCGN_08720 0.36 +0.1 483,705 + HEPCGN_08720 0.39 -0.1 483,764 - HEPCGN_08720 0.83 -1.5 483,824 + +0.7 483,825 - -0.2 483,825 - -0.2 483,825 - +0.3 484,252 + sgrR HEPCGN_08725 0.23 -0.3 484,289 + sgrR HEPCGN_08725 0.25 +0.3 484,500 + sgrR HEPCGN_08725 0.38 +0.4 484,758 - sgrR HEPCGN_08725 0.53 +0.2 484,771 - sgrR HEPCGN_08725 0.54 +0.0
Or see this region's nucleotide sequence