Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tnp-IS3 and yaeF are separated by 61 nucleotides yaeF and nlpE are separated by 29 nucleotides nlpE and arfB are separated by 13 nucleotides arfB and yaeQ overlap by 4 nucleotides
HEPCGN_08030: tnp-IS3 - IS3 family transposase, at 343,007 to 343,318
tnp-IS3
HEPCGN_08040: yaeF - YaeF family permuted papain-like enzyme, at 343,380 to 344,183
yaeF
HEPCGN_08045: nlpE - envelope stress response activation lipoprotein NlpE, at 344,213 to 344,923
nlpE
HEPCGN_08050: arfB - aminoacyl-tRNA hydrolase, at 344,937 to 345,359
arfB
HEPCGN_08055: yaeQ - Uncharacterized protein YaeQ, at 345,356 to 345,901
yaeQ
Position (kb) 344
345 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 343.431 kb on - strand at 343.462 kb on + strand, within yaeF at 343.538 kb on - strand, within yaeF at 343.648 kb on - strand, within yaeF at 343.658 kb on + strand, within yaeF at 343.724 kb on + strand, within yaeF at 343.725 kb on - strand, within yaeF at 343.811 kb on + strand, within yaeF at 343.853 kb on + strand, within yaeF at 343.879 kb on + strand, within yaeF at 343.879 kb on + strand, within yaeF at 343.947 kb on - strand, within yaeF at 344.049 kb on - strand, within yaeF at 344.069 kb on + strand, within yaeF at 344.070 kb on - strand, within yaeF at 344.070 kb on - strand, within yaeF at 344.076 kb on - strand, within yaeF at 344.113 kb on + strand at 344.114 kb on - strand at 344.118 kb on + strand at 344.119 kb on - strand at 344.120 kb on + strand at 344.120 kb on + strand at 344.145 kb on + strand at 344.254 kb on + strand at 344.276 kb on + strand at 344.277 kb on - strand at 344.317 kb on + strand, within nlpE at 344.317 kb on + strand, within nlpE at 344.331 kb on - strand, within nlpE at 344.331 kb on - strand, within nlpE at 344.340 kb on - strand, within nlpE at 344.466 kb on + strand, within nlpE at 344.467 kb on - strand, within nlpE at 344.499 kb on - strand, within nlpE at 344.499 kb on - strand, within nlpE at 344.526 kb on + strand, within nlpE at 344.598 kb on + strand, within nlpE at 344.627 kb on + strand, within nlpE at 344.627 kb on + strand, within nlpE at 344.628 kb on - strand, within nlpE at 344.656 kb on + strand, within nlpE at 344.657 kb on - strand, within nlpE at 344.661 kb on + strand, within nlpE at 344.860 kb on + strand at 344.861 kb on - strand at 344.876 kb on - strand at 345.038 kb on + strand, within arfB at 345.208 kb on + strand, within arfB at 345.208 kb on + strand, within arfB at 345.209 kb on - strand, within arfB at 345.266 kb on + strand, within arfB at 345.266 kb on + strand, within arfB at 345.398 kb on - strand at 345.404 kb on - strand at 345.404 kb on - strand at 345.421 kb on + strand, within yaeQ at 345.421 kb on + strand, within yaeQ at 345.578 kb on - strand, within yaeQ at 345.580 kb on + strand, within yaeQ at 345.580 kb on + strand, within yaeQ at 345.581 kb on - strand, within yaeQ at 345.581 kb on - strand, within yaeQ at 345.581 kb on - strand, within yaeQ at 345.625 kb on + strand, within yaeQ at 345.671 kb on - strand, within yaeQ at 345.753 kb on + strand, within yaeQ at 345.753 kb on + strand, within yaeQ at 345.754 kb on - strand, within yaeQ at 345.878 kb on - strand at 345.880 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 343,431 - +3.3 343,462 + yaeF HEPCGN_08040 0.10 -0.2 343,538 - yaeF HEPCGN_08040 0.20 +0.0 343,648 - yaeF HEPCGN_08040 0.33 +0.2 343,658 + yaeF HEPCGN_08040 0.35 +0.3 343,724 + yaeF HEPCGN_08040 0.43 +0.1 343,725 - yaeF HEPCGN_08040 0.43 -0.4 343,811 + yaeF HEPCGN_08040 0.54 +0.8 343,853 + yaeF HEPCGN_08040 0.59 -2.2 343,879 + yaeF HEPCGN_08040 0.62 +0.0 343,879 + yaeF HEPCGN_08040 0.62 +0.5 343,947 - yaeF HEPCGN_08040 0.71 +0.6 344,049 - yaeF HEPCGN_08040 0.83 +0.1 344,069 + yaeF HEPCGN_08040 0.86 -0.0 344,070 - yaeF HEPCGN_08040 0.86 +0.2 344,070 - yaeF HEPCGN_08040 0.86 +0.3 344,076 - yaeF HEPCGN_08040 0.87 +0.9 344,113 + +0.9 344,114 - +0.7 344,118 + +1.2 344,119 - +0.2 344,120 + +0.1 344,120 + -0.2 344,145 + +0.7 344,254 + -0.1 344,276 + +0.6 344,277 - -1.5 344,317 + nlpE HEPCGN_08045 0.15 +0.3 344,317 + nlpE HEPCGN_08045 0.15 -0.7 344,331 - nlpE HEPCGN_08045 0.17 +0.5 344,331 - nlpE HEPCGN_08045 0.17 -0.2 344,340 - nlpE HEPCGN_08045 0.18 -0.1 344,466 + nlpE HEPCGN_08045 0.36 -2.0 344,467 - nlpE HEPCGN_08045 0.36 +0.2 344,499 - nlpE HEPCGN_08045 0.40 -0.8 344,499 - nlpE HEPCGN_08045 0.40 +0.2 344,526 + nlpE HEPCGN_08045 0.44 -0.4 344,598 + nlpE HEPCGN_08045 0.54 +0.5 344,627 + nlpE HEPCGN_08045 0.58 -0.8 344,627 + nlpE HEPCGN_08045 0.58 -1.3 344,628 - nlpE HEPCGN_08045 0.58 +0.4 344,656 + nlpE HEPCGN_08045 0.62 +0.2 344,657 - nlpE HEPCGN_08045 0.62 +0.3 344,661 + nlpE HEPCGN_08045 0.63 -0.6 344,860 + +0.2 344,861 - +1.2 344,876 - -0.4 345,038 + arfB HEPCGN_08050 0.24 +0.1 345,208 + arfB HEPCGN_08050 0.64 +0.1 345,208 + arfB HEPCGN_08050 0.64 -0.0 345,209 - arfB HEPCGN_08050 0.64 +0.1 345,266 + arfB HEPCGN_08050 0.78 -1.1 345,266 + arfB HEPCGN_08050 0.78 +0.6 345,398 - +0.1 345,404 - +0.1 345,404 - -0.0 345,421 + yaeQ HEPCGN_08055 0.12 +0.5 345,421 + yaeQ HEPCGN_08055 0.12 -0.5 345,578 - yaeQ HEPCGN_08055 0.41 +0.5 345,580 + yaeQ HEPCGN_08055 0.41 +0.6 345,580 + yaeQ HEPCGN_08055 0.41 +0.4 345,581 - yaeQ HEPCGN_08055 0.41 -0.9 345,581 - yaeQ HEPCGN_08055 0.41 +0.3 345,581 - yaeQ HEPCGN_08055 0.41 -0.0 345,625 + yaeQ HEPCGN_08055 0.49 +0.4 345,671 - yaeQ HEPCGN_08055 0.58 -0.6 345,753 + yaeQ HEPCGN_08055 0.73 -0.1 345,753 + yaeQ HEPCGN_08055 0.73 +0.1 345,754 - yaeQ HEPCGN_08055 0.73 +0.1 345,878 - -0.5 345,880 - +0.5
Or see this region's nucleotide sequence