Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rcsF and tsaA overlap by 4 nucleotides tsaA and proS are separated by 110 nucleotides proS and tnp-IS3 are separated by 95 nucleotides tnp-IS3 and HEPCGN_08025 are separated by 128 nucleotides
HEPCGN_08005: rcsF - Rcs stress response system protein RcsF, at 336,968 to 337,372
rcsF
HEPCGN_08010: tsaA - tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO, at 337,369 to 338,076
tsaA
HEPCGN_08015: proS - proline--tRNA ligase, at 338,187 to 339,905
proS
HEPCGN_08020: tnp-IS3 - IS3 family transposase, at 340,001 to 340,441
tnp-IS3
HEPCGN_08025: HEPCGN_08025 - reverse transcriptase domain-containing protein, at 340,570 to 341,745
_08025
Position (kb) 338
339
340 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 337.234 kb on + strand, within rcsF at 337.278 kb on + strand, within rcsF at 337.282 kb on - strand, within rcsF at 337.288 kb on + strand, within rcsF at 337.289 kb on - strand, within rcsF at 337.310 kb on - strand, within rcsF at 337.336 kb on - strand at 337.336 kb on - strand at 337.402 kb on - strand at 337.414 kb on + strand at 337.455 kb on - strand, within tsaA at 337.475 kb on + strand, within tsaA at 337.475 kb on + strand, within tsaA at 337.476 kb on - strand, within tsaA at 337.493 kb on - strand, within tsaA at 337.493 kb on - strand, within tsaA at 337.538 kb on + strand, within tsaA at 337.538 kb on + strand, within tsaA at 337.671 kb on + strand, within tsaA at 337.671 kb on + strand, within tsaA at 337.672 kb on - strand, within tsaA at 337.672 kb on - strand, within tsaA at 337.755 kb on + strand, within tsaA at 337.780 kb on + strand, within tsaA at 337.781 kb on - strand, within tsaA at 337.781 kb on - strand, within tsaA at 337.822 kb on + strand, within tsaA at 337.822 kb on + strand, within tsaA at 337.823 kb on - strand, within tsaA at 337.917 kb on - strand, within tsaA at 337.917 kb on - strand, within tsaA at 337.937 kb on + strand, within tsaA at 337.968 kb on + strand, within tsaA at 337.969 kb on - strand, within tsaA at 337.969 kb on - strand, within tsaA at 337.969 kb on - strand, within tsaA at 338.017 kb on - strand at 339.896 kb on + strand at 339.954 kb on + strand at 339.955 kb on - strand at 339.996 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 337,234 + rcsF HEPCGN_08005 0.66 +0.1 337,278 + rcsF HEPCGN_08005 0.77 +0.1 337,282 - rcsF HEPCGN_08005 0.78 +0.4 337,288 + rcsF HEPCGN_08005 0.79 +0.0 337,289 - rcsF HEPCGN_08005 0.79 -1.3 337,310 - rcsF HEPCGN_08005 0.84 +0.0 337,336 - +0.1 337,336 - +0.7 337,402 - +0.3 337,414 + -1.1 337,455 - tsaA HEPCGN_08010 0.12 -0.2 337,475 + tsaA HEPCGN_08010 0.15 -0.2 337,475 + tsaA HEPCGN_08010 0.15 +0.0 337,476 - tsaA HEPCGN_08010 0.15 -0.4 337,493 - tsaA HEPCGN_08010 0.18 +0.1 337,493 - tsaA HEPCGN_08010 0.18 +0.0 337,538 + tsaA HEPCGN_08010 0.24 +1.2 337,538 + tsaA HEPCGN_08010 0.24 -0.9 337,671 + tsaA HEPCGN_08010 0.43 +0.5 337,671 + tsaA HEPCGN_08010 0.43 +0.2 337,672 - tsaA HEPCGN_08010 0.43 -0.5 337,672 - tsaA HEPCGN_08010 0.43 -0.1 337,755 + tsaA HEPCGN_08010 0.55 -0.3 337,780 + tsaA HEPCGN_08010 0.58 +0.3 337,781 - tsaA HEPCGN_08010 0.58 -0.3 337,781 - tsaA HEPCGN_08010 0.58 +0.0 337,822 + tsaA HEPCGN_08010 0.64 -0.1 337,822 + tsaA HEPCGN_08010 0.64 +0.1 337,823 - tsaA HEPCGN_08010 0.64 -6.0 337,917 - tsaA HEPCGN_08010 0.77 +0.7 337,917 - tsaA HEPCGN_08010 0.77 +0.8 337,937 + tsaA HEPCGN_08010 0.80 +0.0 337,968 + tsaA HEPCGN_08010 0.85 +0.1 337,969 - tsaA HEPCGN_08010 0.85 +0.6 337,969 - tsaA HEPCGN_08010 0.85 +0.1 337,969 - tsaA HEPCGN_08010 0.85 +0.7 338,017 - +0.1 339,896 + +0.9 339,954 + +1.0 339,955 - +1.0 339,996 - +0.3
Or see this region's nucleotide sequence